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Biomedical subjects

J Ouyang

Publications and source records attributed to J Ouyang.

At least 37 records · Page 2Linked to original sources
PubMed2002

Increases in muscle MCT are associated with reductions in muscle lactate after a single exercise session in humans.

To investigate the effects of a single session of prolonged cycle exercise [60% peak O2 uptake (VO2 peak) for 5-6 h] on metabolic adaptations in working vastus lateralis muscle, nine untrained males (peak O2 uptake = 47.2 +/- 1.1 ml x kg(-1) x min(-1), means +/- SE) were examined before (Pre) and at 2 (Post-2), 4 (Post-4), and 6 (Post-6) days after the training session. On the basis of 15 min of cycle exercise at 59% VO2 peak, it was found that training reduced (P < 0.05) exercise muscle lactate (mM) at Post-2 (6.65 +/- 0.69), Post-4 (7.74 +/- 0.63), and Post-6 (7.78 +/- 1.2) compared with Pre (10.9 +/- 1.3). No effect of training was observed on exercise ATP, phosphocreatine, and glycogen levels. After the single session of training, plasma volumes were elevated (P < 0.05) at Post-2 (6.7 +/- 1.7%), Post-4 (5.86 +/- 1.9), and Post-6 (5.13 +/- 2.5). The single exercise session also resulted in elevations (P < 0.05) in the monocarboxylate transporters MCT1 and MCT4 throughout the 6 days after exercise. Although epinephrine and norepinephrine both increased with exercise, only norepinephrine was reduced (P < 0.05) with training and only at Post-4. These results indicate that regulation of cellular lactate levels occurs rapidly and independently of other metabolic adaptations. It is proposed that increases in MCT and plasma volume are at least partly involved in the lower muscle lactate content observed after the training session by increasing lactate membrane transport and removal, respectively.

Adult↗
PubMed2001

Active site analysis of the potential antimicrobial target aspartate semialdehyde dehydrogenase.

Aspartate-beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in the biosynthetic pathway through which bacteria, fungi, and the higher plants synthesize amino acids, including lysine and methionine and the cell wall component diaminopimelate from aspartate. Blocks in this biosynthetic pathway, which is absent in mammals, are lethal, and inhibitors of ASADH may therefore serve as useful antibacterial, fungicidal, or herbicidal agents. We have determined the structure of ASADH from Escherichia coli by crystallography in the presence of its coenzyme and a substrate analogue that acts as a covalent inhibitor. This structure is comparable to that of the covalent intermediate that forms during the reaction catalyzed by ASADH. The key catalytic residues are confirmed as cysteine 135, which is covalently linked to the intermediate during the reaction, and histidine 274, which acts as an acid/base catalyst. The substrate and coenzyme binding residues are also identified, and these active site residues are conserved throughout all of the ASADH sequences. Comparison of the previously determined apo-enzyme structure [Hadfield et al. J. Mol. Biol. (1999) 289, 991-1002] and the complex presented here reveals a conformational change that occurs on binding of NADP that creates a binding site for the amino acid substrate. These results provide a structural explanation for the preferred order of substrate binding that is observed kinetically.

Aspartate-Semialdehyde Dehydrogenase↗
PubMed2001

[A preliminary result of treatment of progressive multiple sclerosis with autologous peripheral blood stem cell transplantation in China].

OBJECTIVE: To explore the clinical effect for treatment of progressive multiple sclerosis (PMS) by autologous peripheral blood stem cell transplantation (Auto-PBSCT). METHODS: One PMS patient was chosen. Peripheral blood stem cells (PBSCs) were mobilized with cyclophosphamide and G-CSF. The CD34+ cells harvesting was performed by continuous flow leukapheresis using a CS3000 plus blood separator. About 12.2 x 10(6) CD34+ cells/kg was collected and preserved at -196 degrees C in liquid nitrogen. PBSCs were reinfused after conditioned by CTX (120 mg/kg) and TBI(10 GY). G-CSF was used to help hematopoietic and immunologic reconstitution. RESULTS: After transplantation the patient received clinical remission, and the abnormal signals of MRI in T1-T4 disappeared, the examination results of the neuro-electrophysiology and T cell subgroup were improved obviously. There was no relapse after 6 months follow up. CONCLUSIONS: Auto-PBSCT is effective and safety for PMS. Duration of remission remains to be decided in long-term follow up.

Adult↗
PubMed2001

[High level secretion expression of porcine somatoropin gene in Pichia pastoris and N-glycosylation analysis of products].

The porcine somatoropin gene was inserted into the Pichia pastoris expression vector of pPICZ alpha A which contains AOX I promoter and alpha-factor signal sequence. The recombinant plasmid of pPICZ alpha A-pST was linearnized by Sac I and transformed into X-33 by electroporation. The multi-copy insert transformants were selected and cultivated in flasks. SDS-PAGE and Western blot analysis showed that PST gene products were observed in the supernants with a little larger molecular weight size than the natural PST's, however, the molecular weight size of the PST gene products in the soluble cellular proteins were identical to the natural PST's. Retransformation of the linearnized pPICZ alpha-pST showed the expression level was improved greatly and rPST has the same antigenicity as natural one. The expressed rPST accumulated up to about 956 mg/L. The N-glycosylation analysis showed rPST had no N-glycosylation.

Animals↗
PubMed2001

[A pilot study in treatment of systemic lupus erythematosus by autologous bone marrow transplant].

OBJECTIVE: To explore the clinical effect for treatment of SLE by autologous bone marrow transplantation(ABMT). METHODS: Three patients with refractory SLE were chosen. BM stem cells were harvested from the posterior superior iliac crests under epidural anesthesia and preserved in 4 degrees C. BM stem cells were reinfused after conditioned by CTX (120 mg/kg) and malphalan (140 mg/m(2)) or VP-16 (1.0 g/m(2)). G-CSF was used to help hematopoietic and immunologic reconstitution. RESULTS: These three patients had clinical remission after transplantation; the levels of immunoglobulins declined and complements rose up. Most of the auto-antibodies turned negative. Skin pathology in case 2 showed that the former tissue injury alleviated or disappeared. Moreover, myocardial hypertrophy and pericardial effusion also disappeared. CONCLUSIONS: (1) ABMT is obviously effective for SLE. The duration of remission remains to be decided in long-term follow up. (2) Mechanisms of improvement are possibly related to decrease of immunologic pathological cells in tissues after conditioning, reduction of the level of immunoglobulins and drop of the quantity of auto-antibodies.

Adolescent↗
PubMed2000

Measurement of the spin asymmetry in the photoproduction of pairs of high- p(T) hadrons at HERMES

We present a measurement of the longitudinal spin asymmetry A(||) in photoproduction of pairs of hadrons with high transverse momentum p(T). Data were accumulated by the HERMES experiment using a 27.5 GeV polarized positron beam and a polarized hydrogen target internal to the HERA storage ring. For h(+)h(-) pairs with p(h(1))(T)>1.5 GeV/c and p(h(2))(T)>1.0 GeV/c, the measured asymmetry is A(||) = -0. 28+/-0.12(stat)+/-0.02(syst). This negative value is in contrast to the positive asymmetries typically measured in deep inelastic scattering from protons, and is interpreted to arise from a positive gluon polarization.

Journal Article↗
PubMed2000

Spectral behavior and pH dependence of N-hexadecyl-5-iminomethyl-8-hydroxyquinoline.

A new kind of amphiphile derived from 8-hydroxyquinoline, N-hexadecyl-5-iminomethyl-8-hydroxyquinoline (HIHQ) was synthesized and characterized by different physical methods. The influence of pH value on UV-vis absorption, fluorescence spectra were investigated and the surface pressure area isotherms were recorded under different pH subphase. The linear response range between the emission intensity and the concentration of HIHQ was 1-4 x 10(-6) mol L(-1). The strongest emission of HIHQ about 460 nm was obtained at pH 8.2 and the acid-base equilibrium of HIHQ in monolayer and solution was also discussed.

Hydrogen-Ion Concentration↗
PubMed2000

Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana.

The phytohormone indole-3-acetic acid (IAA) plays a vital role in plant growth and development as a regulator of numerous biological processes. Its biosynthetic pathways have been studied for decades. Recent genetic and in vitro labeling evidence indicates that IAA in Arabidopsis thaliana and other plants is primarily synthesized from a precursor that is an intermediate in the tryptophan (Trp) biosynthetic pathway. To determine which intermediate(s) acts as the possible branchpoint for the Trp-independent IAA biosynthesis in plants, we took an in vivo approach by generating antisense indole-3-glycerol phosphate synthase (IGS) RNA transgenic plants and using available Arabidopsis Trp biosynthetic pathway mutants trp2-1 and trp3-1. Antisense transgenic plants display some auxin deficient-like phenotypes including small rosettes and reduced fertility. Protein gel blot analysis indicated that IGS expression was greatly reduced in the antisense lines. Quantitative analyses of IAA and Trp content in antisense IGS transgenic plants and Trp biosynthetic mutants revealed striking differences. Compared with wild-type plants, the Trp content in all the transgenic and mutant plants decreased significantly. However, total IAA levels were significantly decreased in antisense IGS transgenic plants, but remarkably increased in trp3-1 and trp2-1 plants. These results suggest that indole-3-glycerol phosphate (IGP) in the Arabidopsis Trp biosynthetic pathway serves as a branchpoint compound in the Trp-independent IAA de novo biosynthetic pathway.

Arabidopsis↗
PubMed2000

Effects of a 21-day expedition to 6,194 m on human skeletal muscle SR Ca2+-ATPase.

We investigated the effects of a 21-day expedition to the summit of Mount Denali, Alaska (6,194 m) on selected Ca2+ sequestration properties of sarcoplasmic reticulum (SR) calcium pump in vastus lateralis muscle. Muscle samples were obtained by biopsy from 5 male climbers (peak oxygen consumption, VO2peak = 52.3 +/- 2.1 mL.kg(-1).min(-1)) approximately 7 days prior to (PRE) and 4 days following (POST) the expedition. A comparison of PRE versus POST measures of maximal Ca2+-ATPase activities (117 +/- 8.5 vs. 97.6 +/- 5.6 nmol.mg protein(-1).min(-1)) and Ca2+-uptake (204 +/- 15 vs. 161 +/- 11 nmol.mg protein(-1).min(-1)) measured in crude homogenates obtained from pre-exercised muscle, indicated only an effect (p < 0.05) of the expedition on Ca2+-uptake. The reduction in Ca2+-ATPase activity, representing 16.6%, was not significant (p = 0.089). The sarco endoplasmic reticulum calcium (SERCA)-ATPase isoforms, measured using Western blotting techniques, revealed a small reduction (p < 0.05) in SERCA 1 (-4.6 +/- 1.9%), but not in SERCA 2a (+2.0 +/- 1.4%). Prior to the expedition, both Ca2+-ATPase activity and Ca2+-uptake were reduced (p < 0.05) by approximately 34 and 18%, respectively, following 40 min of a two-step continuous cycling task (20 min at 59% VO2peak and 20 min at 74% VO2peak). The exercise-induced reduction in Ca2+-ATPase activity was independent of fiber type. Only in the case of Ca2+-uptake was a lower exercise response (p < 0.05) observed following the expedition, an effect that was due to the lower resting value. It is concluded that acclimatization as experienced during a mountaineering expedition induces changes in the properties of the SR Ca2+-pump, and particularly to Ca2+-sequestering function.

Acclimatization↗
PubMed2000

Cervical venous structure in the inter-transverse and intra-transverse foraminal region: an anatomic study.

Vertebral venous bleeding is frequently encountered during anterolateral cervical decompression. The present study was undertaken to identify the pattern and location of the vertebral vein in relationship to the vertebral artery in the inter-transverse and intra-transverse foraminal regions. Twenty-one cadavers were dissected to determine the anatomic features of the vertebral vein in the inter-transverse and intra-transverse foramina. The vertebral veins in the inter-foraminal and intraforaminal regions from C-3 to C-6 can be classified into three types: single or double veins, venous plexus, and absence of the vein. Of the 21 specimens, vertebral veins were found bilaterally in five specimens (24%) and unilaterally in eight specimens (38%). The veins were situated either anterolateral or anteromedial to the vertebral artery. The venous structures showed in venous plexus in two specimens (9.5%). In six specimens (29%), there were no obvious venous structures related to the vertebral artery in its intra-transverse and inter-transverse foraminal course. The veins are contained in a fibrous and osseous tunnel as they descend through the transverse foramina. Subperiosteal dissection of fibrous tissue from the lateral aspect of the uncinate process after removal of the anterior wall of the transverse foramen may minimize hemorrhages from the vertebral vein in the inter-transverse and intra-transverse foraminal region during resection of the uncovertebraljoint or neural foraminotomy with retraction of the vertebral artery laterally.

Adult↗
PubMed2000

[Multiple patterns of angle closure mechanisms in primary angle closure glaucoma in Chinese].

OBJECTIVE: The mechanism of primary angle closure glaucoma (PACG) in Chinese is studied to establish a new classification system based on the etiology and mechanism of angle closure. METHODS: The anterior chamber angle's configuration and the anatomic structures related to the angle in 126 cases with PACG were observed with ultrasound biomicroscope (UBM), and the mechanisms of angle closure were analyzed by diagnostic treatment. RESULTS: Based on the configuration of the angle and anatomic structures related to the angle as well as the mechanisms of angle closure, PACG could be divided into following subtypes: pure pupillary block angle closure glaucoma [PPB, 48 cases (38.1%)]; pure non-pupillary angle closure glaucoma [PNP, 9 cases (7.1%)]; multiple mechanism angle closure glaucoma [MM, 69 cases (54.8%)]. PPB manifested that the relative position of pupillary margin was located anteriorly, the peripheral iris bombé was due to the pupillary block, and the angle closure was not related to the location and shape of the peripheral iris and ciliary body. PNP showed that the relative position of pupil margin was located posteriorly, the angle closure was not caused by the pupillary block, but caused by anterior located ciliary body and/or thick and anterior located peripheral iris. MM pattern was caused by both pupillary block and non-pupilary block, and most of the cases manifested creeping angle closure. CONCLUSIONS: The angle closure mechanisms of PACG in Chinese are of multiple patterns. Based on the different mechanisms of angle closure in PACG, a new classification system should be established to improve the diagnosis and treatment of PACG.

Adult↗
PubMed1999

Structure of aspartate-beta-semialdehyde dehydrogenase from Escherichia coli, a key enzyme in the aspartate family of amino acid biosynthesis.

Aspartate beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in an essential aspartic biosynthetic pathway found in bacteria, fungi and the higher plants. Mutations in the asd gene encoding for ASADH that produce an inactive enzyme are lethal, which suggests that ASADH may be an effective target for antibacterial, herbicidal and fungicidal agents. We have solved the crystal structure of the Escherichia coli enzyme to 2.5 A resolution using single isomorphous replacement and 3-fold non-crystallographic symmetry. Each monomer has an N-terminal nucleotide-binding domain and a dimerisation domain. The presence of an essential cysteine locates the active site in a cleft between the two domains. The functional dimer has the appearance of a butterfly, with the NADP-binding domains forming the wings and the dimerisation domain forming the body.A histidine residue is identified as a likely acid/base catalyst in the enzymic reaction. Other amino acids implicated in the enzymic activity by mutagenesis are found in the active site region and define the substrate binding pocket.

Amino Acid Sequence↗
PubMed1999

Traumatic instabilities of the cervical spine caused by high-speed axial compression in a human model. An in vitro biomechanical study.

STUDY DESIGN: Traumatic injury of the cervical spine was produced on human cadavers and evaluated with instability tests and radiographs. OBJECTIVE: To relate traumatic injuries of the cervical spine to instability and patterns of traumatic injury to different levels of impact energy. SUMMARY OF BACKGROUND DATA: Data from young human cadavers are rare in traumatic models of the cervical spine, and instabilities caused by axial compression with different impacts remain unknown. METHODS: Fourteen cervical spine specimens (C2-C4) obtained from fresh human cadavers were divided evenly into two groups and subjected to axial compressive impact with 30 J and 50 J impact energy, respectively. Pure moments in flexion-extension, left/right lateral bending, and left/right axial rotation were applied to each specimen before and after trauma. The maximum moment was 2.0 Nm in each case. Ranges of motion and neutral zones were measured using stereophotogrammetry. RESULTS: Ranges of motion and neutral zones for both groups increased after trauma. No bony injury was observed on the radiographs after trauma with 30 J, but motions increased significantly in flexion, extension, and axial rotation. All specimens showed bony injuries after trauma with 50 J, whereas motions continued to increase significantly in all directions. The relative neutral zone values were larger than the corresponding range of motion values, except in flexion-extension after trauma with 50 J. CONCLUSIONS: The injury patterns of the cervical spine were associated with impact energy, and a high level of impact energy could produce either three-column injury or anterior middle-column injury. Instabilities of the cervical spine caused by compressive trauma increased with the level of impact energy. The neutral zone was more sensitive than the range of motion in representing spinal instability, whereas instability testing was more sensitive than radiographs in evaluating traumatic injury of cervical spine.

Adult↗
PubMed1999

Increased association of glycoprotein 120-CD4 with HIV type 1 coreceptors in the presence of complex-enhanced anti-CD4 monoclonal antibodies.

CD4-specific monoclonal antibodies (CG1, CG7, and CG8), which bind with a 5- to 10-fold higher avidity to preformed CD4-gp120 complexes than to CD4, were previously shown to recognize newly identified conformational epitopes in the D1-CDR3 region of CD4. In the current study, these and other complex-enhanced MAbs were tested in three separate assays of HIV-1 coreceptor (CXCR4/CCR5) recruitment. In these assays, the CD4-specific MAbs CG1, -7, and -8 stabilized the association of coreceptor, gp120, and CD4 in trimolecular complexes. In contrast, the gp120-specific, complex-enhanced MAbs 48d and 17b were inhibitory. These data suggest that conformational changes in the CDR3 region of CD4-D1, induced by gp120 binding, may be involved in coreceptor association and thus play a positive role in the HIV-1 cell fusion process.

Antibodies, Monoclonal↗
PubMed1999

Ethanol metabolism is not required for inhibition of LPS-stimulated transcription of inducible nitric oxide synthase.

We examined the effect of inhibition of ethanol metabolism on ethanol-mediated suppression of Escherichia coli endotoxin (LPS-induced upregulation of transcription and release of inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNFalpha) from rat alveolar macrophages (AM) in vivo. Ethanol (3.45 and 5.5 g/kg/IP) and t-butanol (3.7 g/kg, IP), given 30 min before intratracheal administration of LPS (1.0 mg/kg), inhibited the upregulation of iNOS mRNA and protein, determined by competitor equalized RT-PCR and Western immunoblot, respectively, but not TNFalpha mRNA in AM obtained 2 h after LPS administration by bronchoalveolar lavage (BAL). However, ethanol and t-butanol inhibited LPS-stimulated nitrate and nitrite (RNI) and TNFalpha protein in BAL fluid. Pretreatment of rats with 4-methylpyrazole (100 mg/kg, IP) 2 h before, or disulfiram 30 min before, administration of ethanol (3.45 g/kg, IP) failed to attenuate the inhibitory effect on iNOS mRNA or protein. t-Butyl hydroperoxide (100 mg/kg, IP) given to rats 30 min before administration of LPS enhanced LPS-mediated upregulation of iNOS mRNA and TNFalpha protein in AM and BAL fluid. The inhibitory effect of ethanol on iNOS mRNA was not mediated by an interaction with elevated levels of circulating corticosterone because pretreatment of rats with RU-38486 (100 mg/kg, IM), which inhibited prednisolone (50 mg/kg, IM), induced suppression of LPS-stimulated iNOS mRNA, and failed to attenuate ethanol-mediated inhibition of LPS-stimulated iNOS mRNA in AM. We conclude that metabolism of ethanol to acetaldehyde via alcohol dehydrogenase is not required for ethanol-mediated suppression of LPS-induced iNOS transcription and TNFalpha synthesis/release in AM. Moreover, an interaction of ethanol or acetaldehyde with circulating corticosterone is not involved in ethanol-mediated attenuation of LPS-stimulated iNOS mRNA or protein or TNFalpha protein in the lung. Speculatively, because oxidation of t-butanol to t-butylhydroperoxide results in activation, rather than inhibition, of iNOS and TNF-alpha, the reported ethanol-mediated enhancement of iNOS mRNA may result from the action of the hydroxyethyl radical.

Alcohol Dehydrogenase↗
PubMed1999

Regulation of fiber size, oxidative potential, and capillarization in human muscle by resistance exercise.

To examine the hypothesis that increases in fiber cross-sectional area mediated by high-resistance training (HRT) would result in a decrease in fiber capillarization and oxidative potential, regardless of fiber type, we studied six untrained males (maximum oxygen consumption, 45.6 +/- 2.3 ml. kg-1. min-1; mean +/- SE) participating in a 12-wk program designed to produce a progressive hypertrophy of the quadriceps muscle. The training sessions, which were conducted 3 times/wk, consisted of three sets of three exercises, each performed for 6-8 repetitions maximum (RM). Measurements of fiber-type distribution obtained from tissue extracted from the vastus lateralis at 0, 4, 7, and 12 wk indicated reductions (P < 0.05) in type IIB fibers (15.1 +/- 2.1% vs. 7.2 +/- 1.3%) by 4 wk in the absence of changes in the other fiber types (types I, IIA, and IIAB). Training culminated in a 17% increase (P < 0.05) in cross-sectional area by 12 wk with initial increases observed at 4 wk. The increase was independent of fiber type-specific changes. The number of capillaries in contact with each fiber type increased by 12 wk, whereas capillary contacts-to-fiber area ratios remained unchanged. In a defined cross-sectional field, HRT also increased the capillaries per fiber at 12 wk. Training failed to alter cellular oxidative potential, as measured by succinic dehydrogenase (SDH) activity, regardless of fiber type and training duration. It is concluded that modest hypertrophy induced by HRT does not compromise cellular tissue capillarization and oxidative potential regardless of fiber type.

Adult↗
PubMed1999

Ethanol inhibits lung clearance of Pseudomonas aeruginosa by a neutrophil and nitric oxide-dependent mechanism, in vivo.

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can be found in individuals in which the immune system has been suppressed by HIV/AIDS or chronic alcoholism. We evaluated the role of inducible nitric oxide synthase (NOS II) as a modulator of lung concentrations of P. aeruginosa in normal rats and rats given a single dose of ethanol (ETOH). Rats were pretreated with either sterile saline (PBS, 0.1 ml/kg, i.v.) or the NOS II inhibitor L-N6-iminoethyl lysine (LNIL, 10 mg/kg, i.v.) 15 min before intraperitoneal administration of either PBS (4.5 ml/kg) or ETOH (4.5 g/kg). Thirty min after administration of PBS or ETOH the rats were placed in inhalation chambers and exposed to 45 min of an aerosol containing P. aeruginosa (5 x 10(4) colony forming units, CFU). A group of rats (n = 5-6/treatment/time period) were killed immediately (0 hr) or 4 hr after inhalation of P. aeruginosa. The lungs were homogenized and the P. aeruginosa were grown in nutrient broth to determine the number of viable CFU remaining in the lung. The NOS II and TNFalpha mRNA and protein content lung alveolar macrophages (AM) and neutrophils (PMN) were measured with RT-PCR and Western blot. The concentration of nitrate and nitrite anion in the bronchoalveolar lavage fluid (BALf) and ex vivo incubates of PMN were also measured. The CFU of P. aeruginosa present in the lungs of the four groups of rats at 0 hr did not differ. The CFU of P. aeruginosa in the lung increased (p < 0.05) in rats pretreated with ETOH when compared with that obtained from rats pretreated with PBS. However, pretreatment of rats with LNIL decreased (p < 0.05) the 4 hr lung content of P. aeruginosa. Coadministration of LNIL and ETOH to rats augmented the CFU of P. aeruginosa in lungs to amounts which did not differ from that of rats pretreated with ETOH. Inhalation of P. aeruginosa increased NOS II mRNA and protein in rat AM and PMN. Pretreatment of rats with ETOH alone, or in combination with LNIL, inhibited P. aeruginosa-induced NOS II transcription and translation and AM and PMN nitrate and nitrite generation whereas pretreatment with LNIL alone only inhibited nitrate and nitrite generation. Pretreatment of rats with ETOH suppressed P. aeruginosa stimulated PMN recruitment into the lung whereas LNIL enhanced (p < 0.05) P. aeruginosa-stimulated PMN recruitment into the lung. ETOH-induced increases of the lung content of P. aeruginosa were associated with increased PKC delta isozyme in the membrane of the PMN but could not be explained by altered plasma concentrations of hydrocortisone or ETOH. The data demonstrate that selective inhibition of NOS II-derived NO by LNIL decreases the lung content of P. aeruginosa whereas ETOH inhibits the lung clearance of P. aeruginosa. Speculatively, the difference between these effects of LNIL and ETOH may result from differences in drug-induced changes in lung recruitment of PMN.

Animals↗