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Biomedical subjects

J Ouchi

Publications and source records attributed to J Ouchi.

At least 37 records · Page 2Linked to original sources
PubMed1979

Electron microscopical and histochemical studies of outer hair cells in acoustically exposed rabbits.

After acoustic exposure, in the infranuclear region of the basal turn histochemically the lactate dehydrogenase activity increased more than normal. In the lower half of the second turn of this region, small vesicles, free ribosomes and coated vesicles decreased in number and cytoplasmic matrix become lower in electron density and lactic dehydrogenase activity decreased. Small vesicles were found to be preferentially distributed in the presynaptic side of the afferent nerve endings. Movement of these small vesicles leads to the speculation that small vesicles have close relationship to the afferent nerve endings.

Animals↗
PubMed1978

Electron microscopical and histochemical studies of outer hair cells in normal rabbits.

The outer hair cells of the rabbit were electron microscopically and histochemically investigated. The results clearly showed that the outer hair cells of the rabbit have no agglomeration of mitochondria in its infranuclear region. This anatomical fact means that the functional mechanism in the outer hair cells of the rabbit may be substantially different from that of other animals which have agglomeration of mitochondria. From the results of the histochemical study, it is reasonably supposed that the anaerobic energy metabolism may ordinarily play the leading part in sustaining the cellular functions in the infranuclear region of the outer hair cells of the rabbit.

Anaerobiosis↗
PubMed1978

Changes of the stria vascularis induced by ethacrynic acid.

Histological changes in the stria vascularis of guinea pigs after the injection of ethacrynic acid (50 mg/kg) were investigated using a transmission electron microscope and a scanning electron microscope. The changes in permeability in the stria vascularis was also observed using horseradish peroxidase as a tracer. The pathological changes originating in endothelial cells of stria blood vessels and intermediate cells spread into the marginal cells and basal cells. The most remarkable changes were observed one hour after the injection of ethacrynic acid. Subsequently, recovery began in the cells around the blood capillaries and was most complete 3--4 hours after the injection. It is presumed that the strial damages by the ethacrynic acid are caused not only by the direct effect of this drug on the strial cells but also by the disturbances of blood flow and permeability of the capillaries.

Animals↗
PubMed1978

Potentiation and its mechanism of cochlear damage resulting from furosemide and aminoglycoside antibiotics.

The severe ototoxic interaction of the combined administration of furosemide and aminoglycoside antibiotics (kanamycin, streptomycin and gentamicin) was studied in rabbits, and its mechanism clarified. Severe damage occurred not only in the inner ear but also in the kidney when both furosemide and aminoglycoside antibiotics were administered to rabbits. Kanamycin concentration after a single injection of kanamycin with furosemide was much higher not only in the perilymph but also in the cerebrospinal fluid and serum than that after a single injection of kanamycin alone. The ototoxic interaction following the combined use of furosemide and aminoglycoside antibiotics seems to be caused mainly by the inhibitory effect of furosemide on the excretion of aminoglycoside antibiotics from the kidneys.

Aminoglycosides↗
PubMed1978

A detection method for lactic dehydrogenase activity in the inner ear.

A method for the detection of lactic dehydrogenase enzymatic activity in outer hair cells of the rabbit is described. The membranous labyrinth with temporal bone was prefixed in glutaraldehyde. After being placed into the incubation medium, it was postfixed in osmium tetroxide. Specimens of the organ of Corti were removed. Then the specimens were embedded in water-soluble glycol and cut with a cryostat for light microscopy, and also they were embedded in Epon and cut for light and electron microscopy. Sectioning of the membranous labyrinth was very easily made when the specimens were embedded in both the water-soluble glycol and the Epon. The structures of the frozen sections as well as the Epon-embedded ones were well preserved. In the frozen sections the preservation and localization of reaction products were thoroughly kept, but monoformazan of the Epon-embedded sections was soluble.

Animals↗
PubMed1973

[Tympanoplasty].

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Humans↗