Search PubMed⌕ Search

Biomedical subjects

J Ormos

Publications and source records attributed to J Ormos.

At least 91 records · Page 5Linked to original sources
PubMed1978

[X-ray microanalysis of Michaelis-Gutmann bodies with the use of EDAX].

X-ray microanalysis of Michaelis--Gutmann bodies in human malakoplakia (kidney and testes) and in that of rats induced experimentally by administration of endotoxin of Escherichia coli, was carried out. The presence of calcium could be revealed in every Michaelis--Gutmann body according to the lines of Kalfa and K3 as well. The amount of it was in correlation with the stage of the calcification. In the Michaelis--Gutmann bodies found in the rat kidney, fixed without OsO4 presence of P could also be demonstrated. The correlation between the weight per cent of Ca and P seems to evidence the presence of CaHPO4. Results of the X-ray microanalysis of Michaelis--Gutmann bodies found in human and in experimentally induced malakoplakia appeared to be similar.

Antitoxins↗
PubMed1978

X-ray microprobe analysis of Michaelis-Gutmann bodies in human and experimental malakoplakia.

Using x-ray microprobe analysis, we studied Michaelis-Gutmann bodies (M-G bodies) in malakoplakia tissue samples from human kidney and testis as well as from lesions experimentally induced in the rat. In all the M-G bodies tested we could unambiguously detect calcium at both its Kalpha and Kbeta lines. The actual amount of calcium depended on the developmental state, ie, on the degree of calcification of the M-G body, In M-G bodies of rat kidney malakoplakia tissue fixed without osmium tetroxide, the phosphorus could also be detected unambigously. From the ratios of calcium and phosphorus percentages (w/w) we concluded that CaHPO4 was present. No difference was found between M-G bodies in malakoplakia tissues of human origin and those in experimentally induced malakoplakia.

Animals↗
PubMed1977

Postmortem diagnostics of renal diseases from semithin sections.

For the light microscopic postmortem study of mostly glomerular renal diseases, in addition to the paraffin technique, 0.5 mu thick (semithin) sections from material fixed in buffered formaldehyde and embedded in methacrylate or Durcupan ACM were used. The method allows for eventual electron microscopic examinations. The semithin sections were stained with methylene blue combined with basic fuchsin, as well as with periodic acid-silver methamine. The method is not a substitution, but the supplementation of the paraffin technique and is suited for the clarification of numerous fine details: in some cases the exact diagnosis was made in this way.

Cytological Techniques↗
PubMed1977

[Ultrathin sections in postmortem morphological diagnosis of kidney diseases].

Authors for the light-microscopic examination of various--mainly glomerular--diseases of kidneys in addition to paraffin section have used also ultrathin sections (0,5 mu). Material was fixed in buffered-formalin solution and embedded in metacrylate or Durcupan ACM. Sections obtained by this method can be studied by electron micro-scope as well. Sections were stained with methylene-blue and basicfuchsine, further with periodic-acid silver methenamine. The use of ultrathin sections for the light-microscopic studies can not replace the study of paraffin-sections. Ultrathin sections are suitable for the detection of fine details. They can be studied by light- and electron microscopy as well.

Autopsy↗
PubMed1976

Parenchymal destruction and arterial changes (haemodialysis intimal fibrosis) of glomerulonephritic kidneys in chronic intermittent haemodialysis.

The kidneys of 13 chronic diffuse glomerulonephritis patients who had been subjected to chronic intermittent haemodialysis were compared with those of 6 patients treated in the conservative way. Their renal parenchyma was examined with a semiquantitative histological method and advanced complete destruction of the glomeruli was demonstrated in the dialysed cases. There was no perceptive difference in the distribution of the various grades of damage of the recognizable glomeruli and tubules. With the exception of the chronic lobular glomerulonephritis cases, a marked obliterative mucoid intimal proliferation ("haemodialysis-intimal-fibrosis") had developed in the dialysed glomerulonephritis patients. It affected the renal arteries of all size from the segmental to the interlobular arties. Haemodialysis-intimal-fibrosis did not develop in the additionally examined 13 dialysed chronic pyelonephritis cases nor in 8 such treated conservatively. The change seems to be characteristic, but not specific, for chronic diffuse glomerulonephritis treated by chronic intermittent haemodialysis.

Adolescent↗
PubMed1975

Electron microscopic and cytochemical study of the vacuoles of regenerating renal tubular cells.

The structure and acid phosphatase activity of vacuoles observed in regenerating proximal renal tubules were examined ultrastructurally. The sequestration and degradation of organelles were followed in the vacuoles, which obtain the acid phosphatase presumably from the lysosomes, and the limiting membrane of which originates from the endoplasmic reticulum. The vacuoles are regarded as autophagic vacuoles. Their appearance is correlated with the commencing differentiation of the dedifferentiated regenerating cells, which have similar regenerative phenomena.

Acid Phosphatase↗
PubMed1975

Histochemical examination of lysosomal exzymes in necrotic proximal renal tubules of albino rats.

The lysosomal enzymatic activity of the necrotic proximal tubules was examined by light microscopy and electron microscopy in 24- and 48-h focal renal cortical necrosis induced by administration of oestrogen and posterior pituitary extract in rats. Organelles exhibiting acid phosphatase activity can also be seen in the necrotic cells but these differ in size and structure from the lysosomes of normal cells. The cytoplasmic nonspecific esterase and thioacetic acid hydrolase activities decrease considerably or disappear, although some morphologically damaged, but active, lysosomes can be observed. The role of thelysosomal enzymes is seen not in the development of the necrosis but rather in the breaking down of the already necrotic cell constituents.

Acid Phosphatase↗
PubMed1975

Experimentally induced malakoplakia.

Malakoplakia was induced experimentally by introducing large amounts of crude endotoxin-antigen comples of 075 Escherichia coli (E. coli 12797 CDC 0 group 75) into the kidneys and testes of rats. First leukocytes, then granulation tissue composed mainly of characteristic macrophages, the so-called Hansemann cells, appeared around the endotoxin-antigen mass. On the eighth day following the injection, deposition of calcium phosphate into the cytosegresomes of macrophages began and acused the formation of the Michaelis-Gutmann bodies necessary for the diagnosis of malakoplakia. The induction of the same process in humans by E coli endotoxin seems to be possible.

Animals↗
PubMed1975

[Experimental malakoplakia in the kidney].

Malakoplakia in kidney of rats was caused experimentally by injection of large quantities of not purified endotoxin-antigen complex of Escherichia coli 0 75 (O. coli 127 97 CDC 0 group 75; American Type Culture Collection, Eight Edition 1968, page 24). The extract in the beginning became surrounded by leukocytes, later by macrophages. The latter then were transformed into characteristic Hansemann cells. From the 8th day on started the depletion of calcium phosphate in the cytosegresomes of macrophages, thus forming the Michaelis-Guttmann bodies, necessary for the diagnosis of malakoplakia. It is believed that complex of endoxin- and antigen of Escherichia coli may take a part - at least in certain cases - in formation of malakoplakia in human.

Animals↗