Search PubMed⌕ Search

Biomedical subjects

J Oliveira

Publications and source records attributed to J Oliveira.

At least 19 recordsLinked to original sources

Quantifying submarine groundwater discharge in the coastal zone via multiple methods.

Submarine groundwater discharge (SGD) is now recognized as an important pathway between land and sea. As such, this flow may contribute to the biogeochemical and other marine budgets of near-shore waters. These discharges typically display significant spatial and temporal variability making assessments difficult. Groundwater seepage is patchy, diffuse, temporally variable, and may involve multiple aquifers. Thus, the measurement of its magnitude and associated chemical fluxes is a challenging enterprise. A joint project of UNESCO and the International Atomic Energy Agency (IAEA) has examined several methods of SGD assessment and carried out a series of five intercomparison experiments in different hydrogeologic environments (coastal plain, karst, glacial till, fractured crystalline rock, and volcanic terrains). This report reviews the scientific and management significance of SGD, measurement approaches, and the results of the intercomparison experiments. We conclude that while the process is essentially ubiquitous in coastal areas, the assessment of its magnitude at any one location is subject to enough variability that measurements should be made by a variety of techniques and over large enough spatial and temporal scales to capture the majority of these changing conditions. We feel that all the measurement techniques described here are valid although they each have their own advantages and disadvantages. It is recommended that multiple approaches be applied whenever possible. In addition, a continuing effort is required in order to capture long-period tidal fluctuations, storm effects, and seasonal variations.

Brazil↗

Biologic and genetic characteristics of Toxoplasma gondii isolates in free-range chickens from Costa Rica, Central America.

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 144 free-range chickens (Gallus domesticus) from Costa Rica was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT), and found in 60 (40.1%) of 144 chickens with titers of 1:5 in 16, 1:10 in 5, 1:20 in 2, 1:40 in 3, 1:80 in 5, and 1:160 or higher in 29. Tissues of all chickens were bioassayed for T. gondii in mice or cats. Hearts and brains of 52 chickens with titers of 1:5 or higher and 16 chickens with doubtful titers were pooled and bioassayed in mice. Tissues from 76 chickens with MAT titers of 1:10 or less were pooled and fed to three T. gondii-free cats. Fecal floats of cats were bioassayed orally in mice but were negative for T. gondii oocysts. T. gondii was isolated by bioassay in mice from 32 chickens with MAT titers of 1:10 or higher. All infected mice from 4 of the 32 isolates died of toxoplasmosis. Genotyping of these 32 isolates using polymorphisms at the loci SAG1, SAG2, SAG3, BTUB and GRA6 revealed five genotypes. Five isolates had type I alleles and one isolate had type III alleles at all loci. The rest 26 isolates contained the combination of type I and II or I and III alleles and were divided into three genotypes. None was found to have genotype II alleles at all five loci. This is the first report of genetic characterization of T. gondii isolates from Costa Rica, Central America.

Agglutination Tests↗

The prevalence of acne in the north of Portugal.

BACKGROUND: Acne is a disease that affects almost all teenagers (60-90%), and it is the pathology exhibiting the highest morbidity in the 10-24 year-olds age group. There are no consistent data at national level by which we can evaluate the preponderance of this pathology in the Portuguese population. OBJECTIVE: To estimate the prevalence of acne in teenage school students (10-12 year-olds). STUDY DESIGN: A study was carried out involving all 8 regions in the north of Portugal. One school per region was selected at random. RESULTS: As expected, the figure we obtained for the prevalence of this pathology was high (82.4%) with males being the most affected. Nevertheless, only 44% of the affected treated their acne, although the face is the part of the body most affected. In the females high androgenization (which is manifested by the presence of hair on the upper lip and on the side of the face) doubles the median of the degree of acne registered. Regional asymmetry was found in relation to information about the disorder. The degrees of acne are higher in schools in the interior. In the more rural areas, the pharmacist still plays an important role in prescribing treatment. CONCLUSION: As a rule, individuals are correctly informed about acne, confirming that the search for treatment and awareness of its value are generally helping to gradually eliminate some of the stigmas from the past.

Acne Vulgaris↗

Cytochemical detection of calcium in a case of calcinosis circumscripta in a dog.

A 9-month-old intact female Serra-da-Estrela dog (giant Portuguese breed) was presented with a 2-month history of lingual lesions. Two hard, nodular, nonpainful lesions were observed on the lateral border of the tongue. Fine-needle aspiration yielded a chalky white material. Hemacolor-stained smears showed an amorphous granular basophilic background with crystal-like structures and granulomatous inflammation consisting of foamy macrophages and giant cells. Cytochemical staining with von Kossa and Alizarin red S confirmed the calcareous nature of the material. The cytologic diagnosis was lingual calcinosis circumscripta; this was later confirmed by histopathologic examination. To our knowledge, this case is the first to document the use of von Kossa and Alizarin red S on cytologic smears to obtain a definitive diagnosis of lingual calcinosis circumscripta.

Animals↗

[Techniques to block the sciatic nerve by a lateral approach through the popliteal fossa].

Lateral approaches to the sciatic nerve through the popliteal fossa have recently been described as useful for providing adequate anesthesia and postoperative analgesia for foot and ankle surgery. Numerous publications have appeared on the approach in recent years, proposing new anatomical landmarks to facilitate location of the nerve, reduce the rate of complications, and increase the rate of success. When the lateral popliteal approach has been compared to other approaches to the sciatic nerve, similar success rates have been observed. However, when this technique is used certain factors must be borne in mind because they can influence both latency time and success. This review describes the lateral popliteal approach, its main variations, the factors that can affect latency time or success, and the possibility of providing continuous analgesia. We also sought to compare this approach to other techniques for blocking the sciatic nerve.

Humans↗

Validation of soft bottom benthic habitats identified by single-beam acoustics.

Acoustic diversity charts were produced for a Portuguese soft bottom mid-shelf area, depth from 30 to 90 m, using a single-beam echo sounder coupled to the acoustic systems QTC VIEW Series IV and V. A similar acoustic pattern was identified by both systems, which, after ground-truth interpretation based in available sediment and biological data, established a preliminary spatial distribution model of the benthic habitats in this coastal area. However, some of the acoustic areas were interpreted using one or very few sediment and benthic samples. A specific validation survey was conducted a posteriori, in which the positioning of the sediment and benthic community sampling sites was based on the acoustic diversity previously identified. The results clearly confirm the benthic habitats distribution model suggested by the acoustic method, indicating a high potential for the use of such approach in the identification and mapping of large-scale soft bottom coastal shelf habitat diversity.

Acoustics↗

Acute and chronic exposition of mice to severe hypoxia: the role of acclimatization against skeletal muscle oxidative stress.

The role of acclimatization and the effect of persistent severe hypoxia (7000 m) were analyzed in mice soleus muscle with respect to oxidative stress (glutathione redox status) and damage markers (TBARS and SH protein groups), NAG and SOD activities and HSP70 expression. Forty mice were divided into one normobaric-normoxic control group and four hypobaric-hypoxic experimental groups (n = 8). One experimental group (1 D) was acutely exposed to a simulated altitude of 7000 m in a hypobaric chamber for 1 day. Another experimental group (ACCL + 1 D) was exposed to a 3 days acclimatization period plus 1 day of hypoxia exposure at 7000 m. The third experimental group (ACCL + 8 D) was exposed to the same acclimatization protocol, remaining 8 subsequent days at 7000 m. The fourth experimental group (8 D) was chronically exposed without acclimatization. ACCL + 1 D showed a significant decrease (p < 0.05) in oxidative stress and damage compared to the 1 D group. Concerning chronic severe hypoxia, acclimatization was truly vital, since 8 D animals died after 5 days of exposure. Oxidative stress and damage markers in ACCL + 8 D tended to gradually increase throughout the 8 days of the hypoxic period. Total SOD activity did not change in 1 D compared to control; however, it increased significantly (p < 0.05) in ACCL + 1 D and ACCL + 8 D. HSP70 expression followed the observed oxidative stress and damage pattern, suggesting a protective role against hypoxia-induced oxidative stress. The present study supports the hypothesis that acclimatization attenuates oxidative stress and damage induced by acute hypoxia, although a trend to a gradually increased oxidative deleterious effect in skeletal muscle seems to occur during persistent severe hypoxia even after a previous acclimatization period.

Adaptation, Physiological↗

Cytomegalovirus disease latent and active infection rates during the first trimester after kidney transplantation.

Cytomegalovirus (CMV) infection is the single most frequent infectious complication in renal transplant recipients. The aim of this study was to determine the incidence of latent and active infections with CMV during the first 3 months after kidney transplantation. From January 2000 to December 2001, 203 consecutive adult renal transplant recipients underwent weekly measurements of pp65 CMV antigen from the 4th to the 12th posttransplantation week. Latent infection (seropositivity) was found in 92% of the population. Primary infection occurred in 4.9% (10 of 203), among whom 66% were previously seronegative patients. Among the primary infection patients, 70% (7 of 10) developed severe disease. The overall incidence of viremia was 69.5%, being more frequent among cadaver recipients (79% vs 59%; P =.02). The overall incidence of CMV disease was 38.4% (78 of 203) with 24.6% classified as severe disease requiring antiviral therapy. In conclusion, our population showed a high prevalence of latent infection with viremia. Not all patients developed clinical disease. Most subjects experienced a mild spectrum of symptoms, probably due to the prospective search for active infection during the major risk period after kidney transplantation.

Adult↗

Aberrant cellular retinol binding protein 1 (CRBP1) gene expression and promoter methylation in prostate cancer.

AIMS: Retinoids are involved in cell growth, differentiation, and carcinogenesis. Their effects depend on cytosolic transport and binding to nuclear receptors. CRBP1 encodes a protein involved in this process. Because altered CRBP1 expression and promoter hypermethylation occur in several tumours, these changes were investigated in prostate tumorigenesis. METHODS: The CRBP1 promoter was assessed by methylation specific polymerase chain reaction on tissue samples from 36 radical prostatectomy specimens (paired normal tissue, adenocarcinoma, and high grade prostatic intraepithelial neoplasia (HGPIN)), 32 benign prostatic hyperplasias (BPHs), and 13 normal prostate tissue samples from cystoprostatectomies. Methylation of DNA extracted from microdissected tissue was examined blindly. CRBP1 expression was assessed by immunohistochemistry on formalin fixed, paraffin wax embedded tissue. RESULTS: Loss of CRBP1 expression was seen in 15 of 36 adenocarcinomas and 18 of 36 HGPINs. Fifteen adenocarcinomas and nine HGPINs showed overexpression, whereas the remainder showed normal expression. BPH displayed normal expression. No significant associations were found between CRBP1 expression and Gleason score or stage. CRBP1 promoter hypermethylation was found in 17 of 36 adenocarcinomas, three of 35 HGPINs, one of 36 normal prostate tissues from the same patients, none of 32 BPHs, and none of 13 normal prostate tissues from cystoprostatectomies. Loss of expression and hypermethylation of CRBP1 were not significantly associated. CONCLUSIONS: Altered CRBP1 expression and hypermethylation are common in prostate carcinoma, although CRBP1 hypermethylation is not an early event in tumorigenesis. Moreover, both adenocarcinoma and HGPIN show frequent CRBP1 overexpression. The molecular mechanisms underlying altered CRBP1 expression in prostate cancer deserve further study.

Adenocarcinoma↗

Concentric quadriceps and hamstrings isokinetic strength in volleyball and soccer players.

AIM: Muscular strength is one of the most important components of sport, both for high performance and for injury prevention. One of the most used methods to assess strength muscle balance between dominant (D)/non-dominant (ND) and antagonist/agonist is isokinetic testing. The main purpose of the present study was to describe and to compare isokinetic strength profiles (peak torque, bilateral strength differences between D/ND leg (BD) and hamstrings/quadriceps (H/Q) ratio) in athletes of different sports and positional roles. METHODS: Twenty-eight elite volleyball players and 47 pro soccer players (goalkeepers, n = 5; full-backs, n = 7; defenders, n = 10; midfielders, n = 15; forwards, n = 10) were evaluated using an isokinetic dynamometer (Biodex-System 2). Maximal gravity corrected concentric peak torque of knee extensor and flexor muscles were measured at angular velocities of 360 degrees x s(-1) (6.28 rad x s(-1)) and 90 degrees x s(-1) (1.57 rad x s(-1)). RESULTS: No significant BD were found between soccer and volleyball players with exception of hamstrings at 90 degrees x s(-1) (soccer: 10.6 +/- 8.0% vs volleyball: 6.9 +/- 5.5%). The H/Q ratio was significantly lower in volleyball players at 90 degrees x s(-1) (D: soccer 57.4 +/- 6.7% vs volleyball 50.4 +/- 7.2%; ND: soccer: 56.1 +/- 8.2% vs volleyball: 50.5 +/- 6.4%). No significant differences were found for BD and H/Q ratio in soccer players of different positional roles. CONCLUSION: In general, soccer and volleyball players do not seem to be different concerning BD although a significant difference was observed in hamstrings at 90 degrees x s(-1). Moreover, our data suggest that specific demands of these sports and the different positional roles in soccer did not induce bilateral leg imbalances. However, sport demands seem to influence isokinetic concentric H/Q ratio.

Adult↗

[Comparison of 4 techniques for internal saphenous nerve block].

OBJECTIVE: To assess the efficacy of 4 techniques for internal saphenous nerve block with 10 mL of 1.5% mepivacaine. METHODS: Eighty ASA I-II patients scheduled for foot (hallux valgus) surgery with combined sciatic and saphenous nerve blocks were randomized to receive the saphenous nerve block by one of the following techniques: a paravenous approach (n = 20), a transsartorial approach (n = 20), a femoral nerve approach in the inguinal region using a nerve stimulator (n = 20), and by subcutaneous infiltration between the tibial tuberosity and the internal gastrocnemius muscle (n = 20). A pressure cuff was placed 10 cm below the knee of all patients. Success was assessed by pin prick inside the ankle 30 minutes after initiation of the block. Tolerance of the pressure cuff and discomfort during performance of the technique were also assessed. RESULTS: The 4 groups were similar as to distribution of males and females and mean weight, age, and height. Blocking the saphenous nerve by way of the femoral nerve in the inguinal region was the most effective approach (success in 95% of patients), significantly better than the other 3 techniques (P < 0.05). The paravenous approach was successful in 60% of cases, the transsartorial approach in 50%, and the subcutaneous infiltration technique in 45%. The pressure cuff was well tolerated by all patients (100%) in whom the femoral nerve approach was used. The cuff was tolerated by 70% in the paravenous approach group, by 65% in the transsartorial approach group, and by 60% in the subcutaneous infiltration group. Patients reported more discomfort during initiation of the blockade in the paravenous approach and subcutaneous infiltration groups than in the femoral nerve or transsartorial approach groups (P < 0.05). CONCLUSION: The femoral nerve approach in the inguinal region, with nerve stimulator, to block the internal saphenous nerve led to a larger number of successful blocks than did the paravenous or transsartorial approaches, or the technique of subcutaneous infiltration between the tibial tuberosity and internal gastrocnemius muscle.

Aged↗

Expression at mRNA level of cytokines and A238L gene in porcine blood-derived macrophages infected in vitro with African swine fever virus (ASFV) isolates of different virulence.

Porcine macrophage cultures were infected with two ASFV isolates of variable virulence and mRNA levels of several relevant macrophage-derived cytokines were quantified by real time PCR. At six hours post infection, a clear enhancement of mRNA expression of TNFalpha, IL6, IL12 and IL15 was observed in macrophages infected with the low virulent ASFV/NH/P68 (NHV) when compared to those infected with the highly virulent ASFV/L60 (L60). The sequence of the A238L gene homologue to the cellular IkappaB was found identical in both viral isolates and its expression at mRNA level was higher in macrophages infected with NHV when compared to macrophages infected with L60. Furthermore our results suggest a negative correlation between the mRNA expression of A238L gene and the mRNA expression of the above mentioned cytokines (with the exception of IL10) in L60 infected macrophages in opposition to the positive correlation (with exception of the IL1) suggested in NHV infection. Overall, our data strongly emphasize that virulence of ASFV isolates may depend on their capacity to regulate the expression of macrophage-derived cytokines relevant for the development of host protective responses by yet unknown mechanisms triggered by the virus at early stages of the cellular infection.

African Swine Fever↗