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PubMedDate not supplied

Forensic application of rapid analysis of carboxyhemoglobin in blood using an oxygen electrode.

Blood carboxyhemoglobin (HbCO) levels in forensic victims were measured by a new and rapid oxygen-electrode method for successive determinations of HHb, HbO2 and HbCO. It was found that the method is applicable to old denatured blood when combined with the cyanmethemoglobin method. Methemoglobin (Met-Hb) in blood was also estimated by subtracting the sum of HHb, HbO2 and HbCO from total hemoglobin (total Hb.) Blood Met-Hb levels in six victims of city gas poisoning were practically negligible, while those in five victims of fire were 13.5-26.3%.

Adult↗

PubMedDate not supplied

Utility of 3-O-methyl-N-acetyl-D-glucosamine, an N-acetylglucosamine kinase inhibitor, for accurate assay of glucokinase in pancreatic islets and liver.

Glucokinase, an enzyme that catalyzes the phosphorylation of glucose, constitutes the key regulatory step in glucose metabolism in pancreatic islets and liver. We found that 3-O-methyl-N-acetyl-D-glucosamine (3-O-methyl-GlcNAc) potently inhibits glucose phosphorylation by N-acetylglucosamine kinase whereas glucokinase is not at all affected by this hexosamine. The addition of 3-O-methyl-GlcNAc to the assay system for glucokinase in rat liver extracts, which contain a high activity of glucokinase (glucose as substrate) relative to N-acetylglucosamine kinase (N-acetyl-D-glucosamine as substrate), affected neither Km nor Vmax values of glucokinase. On the other hand, both Km and Vmax values of glucokinase in rat pancreatic islet extracts, in which N-acetylglucosamine kinase activity is higher than glucokinase activity, were significantly lowered by the use of 3-O-methyl-GlcNAc as an inhibitor of N-acetylglucosamine kinase.

Acetylglucosamine↗