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Biomedical subjects

J O Koehler

Publications and source records attributed to J O Koehler.

5 recordsLinked to original sources
PubMed1978

Urinary cAMP excretion during surgery: an index of successful parathyroidectomy in patients with primary hyperparathyroidism.

Urinary phosphate (Up) and urinary cAMP (UcAMP) excretion were determine in patients undergoing neck exploration for primary hyperparathyroidism in order to evaluate these parameters as indices of successful surgery. UcAMP fell below 1.5 micro mol/g creatinine in all 12 patients in whom single gland removal corrected hypercalcemia and in 0 of 3 patients in whom no parathyroid tissue was found. The mean time to drop below 1.5 was 2.0 +/- 0.8 h (mean /+- SD) from the time of parathyroidectomy. UcAMP fell below 1.5 in only 1 of 6 patients who had multiple enlarged parathyroid glands removed, irrespective of the outcome of surgery. Changes in Up excretion lagged behind UcAMP changes, so that within the time period studied Up fell to varying degrees in only 10 of 15 patients in whom hypercalcemia was corrected. A spurt in UcAMP excretion, possibly reflecting parathyroid hormone release due to manipulation of a parathyroid gland, occurred in 3 patients. The results suggest that an intraoperative fall in UcAMP below 1.5 predicts successful parathyroidectomy and that an intraoperative spurt in UcAMP may provide a clue to the location of abnormal parathyroid tissue.

Calcium↗
PubMed1977

Computed tomography for parathyroid localization.

Computed tomography (CT) of the neck and mediastinum was performed in 10 patients with primary hyperparathyroidism. Nine had undergone previous surgery and were considered localization problems. Of four subsequently proven cervical adenomas, only one unusually large 19-g adenoma was visualized by CT. However, of two subsequently proven anterior mediastinal adenomas, CT scanning was positive in both; a single posterior mediastinal adenoma was not demonstrated. Of the remaining three patients, one was not operated on; in two others no adenoma was found at surgery. CT scanning is recommended prior to neck surgery in all patients with primary hyperparathyroidism to identify adenomas in the anterior mediastinum.

Adenoma↗
PubMed1972

Effect of insulin on protein synthesis in skeletal muscle of an isolated perfused preparation of rat hemicorpus.

A method for perfusion in vitro of a preparation of rat hemicorpus was developed for study of the metabolism of skeletal muscle. The preparation was stable during perfusion, as indicated by maintenance of ATP concentration, perfusion pressure, and oxygen consumption for up to 90 min. The perfused hemicorpus provided the following advantages for study of protein synthesis in skeletal muscle: (a) hormones and substrates reached the muscle cells through an intact capillary bed, and (b) the preparation included the psoas muscle, which was sufficiently large to allow measurements of intermediates in the pathway of protein synthesis and was readily homogenized for preparation of ribosomes and ribosomal subunits. Perfusion of psoas muscle from fasted rats with buffer containing glucose and insulin reduced the concentration of ribosomal subunits and increased phenylalanine incorporation as compared to perfusion with buffer containing glucose alone. In addition, the hormone increased glucose uptake from the perfusate and inhibited release of free fatty acids from the preparation. When the muscle was perfused with buffer that contained glucose and palmitate, the concentration of ribosomal subunits and phenylalanine incorporation were unchanged. Since fatty acid is known to stimulate protein synthesis in heart muscle, these results indicated that rates of protein synthesis in heart, but not in skeletal, muscle would be maintained during fasting or in diabetic animals by increased plasma concentration of fatty acid.

Adenosine Triphosphate↗