Search PubMed⌕ Search

Biomedical subjects

J Novak

Publications and source records attributed to J Novak.

124 records · Page 7Linked to original sources
PubMedDate not supplied

Morphogenetic variability of faradiol monoesters in marigold Calendula officinalis L.

The main compounds of lipophilic extracts of flower heads of marigold (Calendula officinalis L.) are triterpendiol esters, mainly faradiol laurate, faradiol myristate and faradiol palmitate. These faradiol-3-O-monoesters have been quantified for the first time by means of reversed phase HPLC with internal standardisation in different parts of C. officinalis plants, namely ray florets, disk florests, involucral bracts, receptacles, levaes and seeds. The amounts of the esters were highest in ray florets, approximately 10 times lower in disk florets than in the ray florets, and approximately 10 times lower in involucral bracts than in the disk florets. In the leaves only traces of the esters could be detected, and in the receptacles no esters could be detected at all. Quantification in the seed was not possible using this method because of interfering fatty compounds. Concerning the faradiol esters, the dried ray and disk florets only should be preferred as primary products for remedies as demanded in the recently published supplement of the Pharmacopoiea Europaea (1999). Breeding work should focus on varieties with a greater number of ray florets in order to improve the quality of herbal medicinal products derived from marigoid.

Calendula↗
PubMedDate not supplied

Child care.

Explore the source record for details and available documents.

Child↗
PubMedDate not supplied

Expression of the recombinant human immunoglobulin J chain in Escherichia coli.

Selective transport of polymeric (p) immunoglobulins (Ig) of IgA and IgM isotypes into external secretions by pIg receptor-mediated mechanism depends on the incorporation of joining (J) chain into the polymers. Until now, availability of a free J chain for immunological and biophysical studies has been limited to preparations of denatured J chain forms with moderate yield. Here we report that a recombinant J chain (rJ) can be over-expressed as a soluble fusion protein with thioredoxin using a modified vector pET32 in Escherichia coli. An intact J chain was released by digestion with IgA1 protease from Neisseria gonorrhoeae and isolated in a good yield with immunological and biochemical properties similar to those of J chain obtained by chemical cleavage from pIgA.

Amino Acid Sequence↗
PubMedDate not supplied

Failure of three decision rules to predict the outcome of in-hospital cardiopulmonary resuscitation.

The objective of this study was to evaluate three decision-support tools (the Pre-Arrest Morbidity or PAM score, the Prognosis After Resuscitation or PAR score, and the Acute Physiology and Chronic Health Evaluation or APACHE III score) for their abilities to predict the outcomes of in-hospital cardiopulmonary resuscitation (CPR). The medical records of all 656 adult inpatients undergoing CPR during a two-to-three-year period in three large hospitals were retrospectively reviewed, and demographic and clinical variables were abstracted. Of 656 patients undergoing resuscitation, 248 (37.8%) survived the resuscitation attempt long enough to be stabilized (immediate survival), but only 35 (5.3%) survived to discharge. Only 11 patients had PAM scores higher than 8, none of whom survived to discharge; 131 patients had PAR scores above 8, of whom six survived to discharge. The PAR score and the APACHE III score had the greatest areas under the receiver operating characteristic curves (when predicting the outcome of survival to discharge), although no individual area for either outcome was greater than 0.6. None of the decision-support tools studied was able to effectively discriminate between survivors and non-survivors for the outcomes of immediate survival and survival to discharge following in-hospital CPR. This is consistent with previous work utilizing the APACHE II score, which did not identify a threshold above which patients did not benefit from CPR. The findings for the PAR score and the PAM score stand in contrast to previous studies that found them to be potentially useful decision rules. Further work is needed to develop a decision-support tool that better discriminates between survivors and non-survivors of in-hospital CPR.

APACHE↗
PubMedDate not supplied

Tympanometric screening in elementary school children.

The revised ASHA (American Speech Language Hearing Association, 1990) hearing screening guidelines were developed to decrease over-referrals for middle ear disorders through amended tympanometric measurements. These guidelines also recommended a large-scale normative study be done with tympanometric measures to further reduce the over-referral rate. Seven hundred and fifty ears from 392 kindergarten through third-grade children were tested. Normative values of Peak Ytm and tympanometric width were calculated by grade. For increasing grade level, there was an orderly increase in the mean Peak Ytm and a decrease in the mean tympanometric width. Sensitivity and specificity were calculated at 65 and 80% respectively, with a prevalence of 11%. Suggestions for improving sensitivity and specificity are discussed.

Acoustic Impedance Tests↗
PubMedDate not supplied

[Comparative study of induction with propanidid plus succinylcholine and thiopental plus succinylcholine].

A comparative study was carried out in a group of patients on induction obtained by Propanidid + succinylcholine and by Thiopental + succinylcholine. Serum potassium variations were determined before narcosis and 2 and 4 minutes after succinylcholine. The study was done by means of Student's parameter noting unsignificant and significant increases and decreases, comparing the treated lots with the control lot, and the lots in terms of time, therefore the dynamic evolution of the process and the lots in terms of the drugs. It is demonstrated that in the patients with a high potassium intracellular release potential, the Propanidid + succinylcholine combination is more advantageous.

Adult↗