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Biomedical subjects

J Nousiainen

Publications and source records attributed to J Nousiainen.

6 recordsLinked to original sources

Effects of vegetable fats versus lard in milk replacers on feed intake, digestibility, and growth in Finnish Ayrshire bull calves.

The aim was to study whether vegetable fat mixtures could be used instead of lard [15.2% in dry matter (DM)] in milk replacers without impairing the performance of Finnish Ayrshire bull calves (n = 58). The growth performance of the calves was measured before and after weaning from 14 d to 6 mo of age. The following 3 fat sources in a milk replacer were studied: 1) a mixture of palm, coconut, and rapeseed oil, 2) palm and coconut oil, and 3) lard. The calves were bucket-fed 2 L of milk replacer 3 times per day. The milk replacer contained 116 g of DM/L, resulting in an average DM intake of 4.8 g of DM/kg of body weight0.75 (BW0.75) during the 8-wk trial, after which the calves were weaned. All the calves had free access to water, commercial starter, and grass silage before weaning. The weaned calves had free access to water and grass silage and were given 3 kg/d (air-dry basis) of a commercial concentrate mixture. The concentrate was replaced by barley when the bulls were 4.5 mo old. There were no significant differences between the diets in feed intake and apparent diet digestibility. The health and BW of the calves were similar during the study. The feed conversion rate (kg of DM intake/kg of gain) before weaning was significantly greater for the lard diet compared with the 2 vegetable fat mixtures. After weaning, the feed conversion rate was slightly lower for the diet that included the palm, coconut, and rapeseed oil mixture than for the diet that included palm and coconut oil mixture. The study showed that the 2 mixtures consisting solely of vegetable oils were effective dietary components, thus providing 2 alternative fat mixtures of milk replacers, for use instead of lard in formulating commercial calf milk replacers.

Aging↗

Evaluation of milk urea nitrogen as a diagnostic of protein feeding.

An evaluation of milk urea nitrogen (MUN) as a diagnostic of protein feeding in dairy cows was performed using mean treatment data (n = 306) from 50 production trials conducted in Finland (n = 48) and Sweden (n = 2). Data were used to assess the effects of diet composition and certain animal characteristics on MUN and to derive relationships between MUN and the efficiency of N utilization for milk production and urinary N excretion. Relationships were developed using regression analysis based on either models of fixed factors or using mixed models that account for between-experiment variations. Dietary crude protein (CP) content was the best single predictor of MUN and accounted for proportionately 0.778 of total variance [MUN (mg/dL) = -14.2 + 0.17 x dietary CP content (g/kg dry matter)]. The proportion of variation explained by this relationship increased to 0.952 when a mixed model including the random effects of study was used, but both the intercept and slope remained unchanged. Use of rumen degradable CP concentration in excess of predicted requirements, or the ratio of dietary CP to metabolizable energy as single predictors, did not explain more of the variation in MUN (R(2) = 0.767 or 0.778, respectively) than dietary CP content. Inclusion of other dietary factors with dietary CP content in bivariate models resulted in only marginally better predictions of MUN (R(2) = 0.785 to 0.804). Closer relationships existed between MUN and dietary factors when nutrients (CP to metabolizable energy) were expressed as concentrations in the diet, rather than absolute intakes. Furthermore, both MUN and MUN secretion (g/d) provided more accurate predictions of urinary N excretion (R(2) = 0.787 and 0.835, respectively) than measurements of the efficiency of N utilization for milk production (R(2) = 0.769). It is concluded that dietary CP content is the most important nutritional factor influencing MUN, and that measurements of MUN can be utilized as a diagnostic of protein feeding in the dairy cow and used to predict urinary N excretion.

Animal Nutritional Physiological Phenomena↗

Colostral whey concentrate supplement increases complement activity in the sera of neonatal calves.

We evaluated the effect of a commercial bovine colostral whey on the complement-mediated immune responses of calves. Two groups of neonatal calves were fed, in addition to whole milk (WM) and pooled colostrum (PC), different amounts of a commercial immunoglobulin concentrate made from pooled colostral whey (Ig-C) for the first two feedings post natum. The control group was fed WM and PC only. Serum samples were obtained at the ages of 2, 7, 14 and 30 d. Bacteriolytic activity against complement-sensitive Escherichia coli JM103 and opsonic activity against complement-lysis-resistant E. coli IH3080 strains were studied, as well as the levels of C3 complement component and E. coli JM103 specific antibodies in the sera. Groups fed Ig-C had 2-3 times higher bacteriolytic activity than the control group of both the classic (P < 0.005) and alternative pathways (P < 0.0001) at days 2 and 7 post natum. This effect is obviously not caused solely by the antibodies ingested but also involves other unknown colostral factors, possibly lectins. The opsonisation capacities of the sera correlated well with the amounts of immunoglobulins ingested (P < 0.05) at days 2-14. The levels of C3 component in sera did not differ between the groups. In the group fed the largest amount of immunoglobulins levels of E. coli JM103-specific antibodies were highest (P < 0.0001). It can thus be concluded that the antibody independent complement activities of serum can be increased substantially by feeding colostral whey concentrate to calves during their first days of life.

Age Factors↗

Windows software for cardiac electrophysiology studies and ablation monitoring.

A system for cardiac electrophysiology (EP) studies consisting of a Windows software package, a standard 120 MHz Pentium PC with a high-performance video card and a data acquisition card has been developed during this study. The system is capable of real time data acquisition and storage of 24 channels with simultaneous display of 1-16 arbitrarily chosen channels at a sampling rate of 500 Hz. It can be used clinically in electrophysiology studies and during catheter radio-frequency ablation treatment for monitoring the ablation and its effects. The built-in ablation monitoring capability enables combined EP study and ablation treatment, thus helping to reduce exposure times and the total time needed per patient. For clinical use the software includes versatile tools for data analysis and reduction. Our system has been developed in association with Department of Cardiology of Tampere University Hospital and has been in regular clinical use there.

Electrophysiology↗

Normal vector magnetocardiogram. I. Correlation with the normal vector ECG.

The vector magnetocardiogram (VMCG) has been measured with the corrected unipositional VMCG lead system and analyzed statistically in 290 normal subjects. The morphologic study of the QRS waveforms showed that in the right-to-left (X) component, the triphasic qRs waveform appeared in 55% of the subjects. The superoinferior (Y) component was characterized by a prominent S wave in 96% of the subjects, and the anteroposterior (Z) component was also characterized by a prominent S wave in 95% . The VMGs were compared with the vector electrocardiograms (VECG) recorded in a subgroup of 200 subjects, in whom both the VMCG and VECG were available for computer analysis. The normal variability of the spatial vector magnitude measurements was significantly greater in the VMCG than in the VECG. Some similarities were observed in the waveforms of the time-averaged QRS complexes between the VMCG and VECG. Multiple linear regression analysis between the VMCG and VECG showed that maximally 27, 45, and 41% of the variation in the instantaneous QRS X, Y, and Z amplitudes of the VMCG, respectively, could be explained by the instantaneous X, Y, and Z amplitudes of the VECG.

Adolescent↗

Normal vector magnetocardiogram. II. Effect of constitutional variables.

The effects of age, sex, height, weight, and dimensions of the thorax on the vector magnetocardiogram (VMCG) were studied in 290 normal subjects. The effect of sex on 141 VMCG parameters was tested with the Mann-Whitney U test, and the effects of age and body dimensions on the male and female subjects were tested with the Kruskal-Wallis H test. Similar analyses were applied for a comparison of the vector electrocardiogram (VECG) in a subgroup of 200 normal subjects. Sex had the most pronounced effect on the VMCG, a total of 58% of the instantaneous time-normalized QRS and T amplitude parameters were significantly (P < .01) different in men and women (47% for the VECG). In women, the absolute VMCG amplitudes were, on average, about 60% of those in men. In the VECG, the corresponding portion was about 85%. In men and women, 50 and 26% of the time-normalized amplitude parameters, respectively, were significantly (P < .01) affected by age; the main effect was a decrease in most VMCG amplitudes with increasing age. In the VECG, the corresponding effect was not as statistically significant. The effect of constitutional variables of the body on the VMCG and VECG was, in most cases, not statistically significant. Multiple correlation analysis showed that the five noncardiac factors together explained, at most, 16 and 28% of the variability in the instantaneous, time-normalized QRS amplitudes for men and women, respectively. In the VECG, these figures were 13 and 18%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗