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Biomedical subjects

J North

Publications and source records attributed to J North.

At least 73 records · Page 4Linked to original sources
PubMed1988

Use of a cross reacting anti-idiotype to identify normal counterparts of neoplastic cells.

We have established that 9G4, a cross reacting monoclonal anti-idiotype identifies an epitope close the binding site of anti-I cold agglutinins. 9G4 positive lymphocytes comprise around 5% of B cells and are widely represented in all lymphoid tissue. They may be detected in 15 week fetal spleen at the time of the earliest appearance of B cells and thus form part of the basic B cell repertoire. Such antibodies are derived largely from CD5 positive B cells. 9G4 is thus a useful tool to investigate the normal counterpart of the CLL cell.

Adult↗
PubMed1987

Isolation of an antigen-specific T suppressor factor that suppresses the in vivo response of DBA/2 mice to ferredoxin.

A T cell hybridoma (Fd11) has been produced from B10.D2 mice that secretes a putative antigen-specific T suppressor factor (TsF). The TsF is isolable from culture supernatants of Fd11 by affinity purification over columns containing either a monoclonal antibody (B16G) shown previously to be capable of binding murine TsF or ferredoxin (Fd), the nominal antigen to which the Fd11 TsF binds. Specificity of the Fd11 TsF for Fd was established by comparing it to another TsF isolated by us (A10 TsF) in a sandwich ELISA, and by demonstrating the specific reactivity to Fd of the hybridoma in calcium flux studies. The Fd11 affinity-purified TsF was shown to contain two major unique components with m.w. in the region of 80,000 and 35,000 when run on reducing polyacrylamide gels in the presence of sodium dodecyl sulphate. Specific immunosuppressive properties of Fd11 were demonstrated when Fd11 TsF (10 micrograms) was injected i.v. into Fd-primed syngeneic mice at the time of antigen boost. Fd11 TsF specifically and significantly diminished the secondary antibody response to Fd in DBA/2 mice.

Aminoquinolines↗
PubMed1987

Serum acute phase reactants in necrotizing enterocolitis.

The serum acute phase reactants, C-reactive protein (CRP) and orosomucoid, rose significantly within 24 to 48 hours of presentation in infants with definite necrotizing enterocolitis (NEC) compared with the levels in infants with symptoms who tolerated early reintroduction of enteral feeds. A serum CRP value greater than 10 mg/l within 48 hours identified infants with definite NEC with a 92% sensitivity and an 81% specificity. Serum CRP values remained elevated at 7 to 10 days in 4 infants with late septicaemia and in 4 of 5 infants with abscess or early stricture requiring surgery. Serum acute phase reactants are a potentially valuable adjunct to clinical assessment in the management of infants with suspected NEC.

Acute-Phase Proteins↗
PubMed1986

Biochemical analysis of the role of cytoplasmic ribosomes of Coprinus cinereus in cycloheximide resistance.

The development of an optimized in vitro polyuridylic acid-dependent polyphenylalanine-synthesizing system using cell-free extracts of the basidiomycete fungus Coprinus cinereus is described. The in vitro assay has been used to show that cycloheximide-resistant strains CY8.2, CY9.23 and Sp98, all mutant at the cy-2 locus, have cytoplasmic ribosomes which are more resistant to the drug than the corresponding sensitive strains, CY8, CY9 and CY3. Cycloheximide concentrations and molar ratios of cycloheximide to ribosomes required for 50% inhibition in vitro under standard assay conditions are presented for these strains. The molar ratio required for 50% inhibition in vitro is dependent on the concentration of ribosomes in the assay.

Agaricales↗
PubMed1981

[Infection following closed intramedullary nailing by Kuntscher's method in 1059 fractures].

This paper investigates the incidence of infection following Kuntscher intramedullary nailing of 1059 open or closed fractures treated between January 1967 and December 1980. The fractures comprised 503 of the tibia 440 of the femur and 116 of the humerus. The literature over the past 10 years demonstrates that infection following plating or nailing with open reduction is more frequent than after closed intramedullary nailing. This paper confirms that nailing with open reduction for tibial and femoral fractures increases the infection rate to 5.7% and 3.5% respectively. Closed intramedullary nailing by Kuntscher's method is considered to be sound treatment in a university service for the treatment of fractures of the shaft of the tibia, femur and humerus with an infection rate of less than 1% in closed fractures.

Adult↗
PubMed1975

Mediastinitis in a child caused by perforation of pharynx.

Mediastinitis in infants and children secondary to trauma is rarely reported as evidenced by a literature search. A case of mediastinitis occurred following perforation of the pharynx by a pencil. In neonates, traumatic perforation may mimic esophageal atresia. In older children with a history of trauma to the mouth or pharynx by a sharp object, the possibility of secondary mediastinitis exists.

Abscess↗
PubMedDate not supplied

A far off place.

Explore the source record for details and available documents.

Brain Edema↗
PubMedDate not supplied

Eosinophil development and bone marrow and tissue eosinophils in atopic asthma.

BACKGROUND: Eosinophils develop from bone marrow (BM) progenitors, and interleukin-5 (IL-5) and eotaxin may act in expansion and mobilisation of BM eosinophils in asthma. METHODS: We have examined phenotypic changes as CD34+ cells develop to the eosinophil lineage in vitro, and have evaluated BM eosinophils from asthmatic and control subjects for expression of the eotaxin receptor, CCR3. RESULTS: Acquisition of receptors for IL-5 and CCR3 was an early event in eosinophil development. There were increased CD34+ cells, and mature and immature CCR3+ eosinophils in BM from asthmatics. CONCLUSION: These data suggest that IL-5 may act early in eosinophil development, and that eotaxin has the capacity to mobilise a BM eosinophil pool in asthma.

Antigens, CD34↗