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Biomedical subjects

J Noble

Publications and source records attributed to J Noble.

At least 109 records · Page 6Linked to original sources

Gamma delta T cells in murine epithelia: origin, repertoire, and function.

The earliest TCR+ cells to appear during fetal development express products of the gamma and delta loci, and emerge as successive waves of cells bearing different V gamma gene products. These appear to emigrate and seed different epithelia. The TCR repertoire of the first two of these waves, V gamma 3 and V gamma 4, respectively, is extremely restricted. Whether the repertoire of these cells is restricted by selective processes or is shaped by developmental restrictions on rearrangements remains to be determined. These cells may function in surveillance for signals of trauma by recognizing self products induced by cellular stress.

Animals↗

The significance of chondromalacic changes on the patella.

A total of 500 arthroscopies were performed in a 2-year period, with special reference made to the patellofemoral joint. In those with pain thought to be arising from this joint, 63% had chondromalacic changes on the posterior surface of the patella compared with a 45% incidence in those with meniscal pathological findings at arthroscopy. This study demonstrates that those with patellofemoral pain do not always have patellar articular changes, and patellar pathology is often asymptomatic. As a result, treatment directed at these lesions must be used with great caution.

Adult↗

Capitellocondylar total elbow replacement for rheumatoid arthritis.

In all, 23 capitellocondylar total elbow replacements were performed in 18 patients with rheumatoid arthritis. Follow-up was for a mean (range) of 3 (1.5-5) years. Pain relief was achieved in all patients and the postoperative range of movement showed an improvement in all directions. No evidence of radiological loosening has been seen. Complications consisted of a transient ulnar nerve palsy in 15 elbows (65%) and a permanent ulnar nerve palsy in one elbow (4%). In two elbows there was delayed wound healing.

Aged↗

Long-term follow-up of slipped capital femoral epiphysis.

The data on 155 hips in 124 patients who had slipped capital femoral epiphysis were retrospectively reviewed at a mean follow-up of forty-one years after the onset of symptoms. The slips were classified, by the duration of symptoms, as acute, chronic, or acute on chronic. As determined by the head-shaft angle, 42 per cent of the slips were mild; 32 per cent, moderate; and 26 per cent, severe. Reduction was performed in thirty-nine hips, and realignment was done in sixty-five hips. Treatment of chronic slips included symptomatic only in 25 per cent of the hips, a spica cast in 30 per cent, pinning in 24 per cent, and osteotomy in 20 per cent. The Iowa hip-rating and the radiographic classification of degenerative joint disease were determined at follow-up; both worsened with increasing severity of the slip and when reduction or realignment had been done. Osteonecrosis (12 per cent) and chondrolysis (16 per cent) also were more common with increasing severity of the slip and when reduction or realignment had been performed; both led to a poor result. Deterioration over time was most marked with increasing severity of the slip. The natural history of the malunited slip is mild deterioration related to the severity of the slip and complications. Techniques of realignment are associated with a risk of appreciable complications and adversely affect the natural history of the disease. Regardless of the severity of the slip, pinning in situ provided the best long-term function and delay of degenerative arthritis, with a low risk of complications.

Adolescent↗

Primary care internal medicine: a challenging career choice for the 1990s.

A career in primary care internal medicine can be challenging and rewarding, yet during the last decade fewer medical students have selected training in internal medicine. We wish to inform medical students, their advisors, and other physicians about the field of primary care internal medicine. We define the discipline, compare it with traditional internal medicine and with family practice, and describe features of strong primary care internal medicine training programs. We discuss common misconceptions and concerns about training programs and the career and give examples of career paths chosen by graduates of primary care programs. We encourage students to consider the option of primary care internal medicine when making career decisions and provide faculty advisors unfamiliar with primary care internal medicine training programs with a reference resource.

Career Choice↗

Acute urinary retention in a child with appendiceal abscess: diagnostic dilemma.

Rarely do children with appendiceal abscess present with acute urinary retention as the only sign or symptom. This presentation may delay diagnosis and definitive therapy, thereby causing additional morbidity and possible mortality. We report the eighth case of acute urinary retention in a child associated with an appendiceal abscess.

Abscess↗

Patellar resurfacing in total condylar knee arthroplasty. Technique and results.

One hundred thirty-two total condylar knee arthroplasties with a 3-8-year follow-up period were studied prospectively with particular reference to the patellofemoral joint. All patients had significant patellofemoral disease and underwent primary patellar resurfacing. After operation 96% of patients had little or no pain and 98% had functional improvement. There were seven (5%) complications related to the patellofemoral joint. The importance of careful attention to preparation of the patella, the technique of prosthetic implantation and correct patellar tracking were emphasized. Routine patellar resurfacing proved highly successful with minimal complications.

Adult↗

Polyoma virus small tumor antigen pre-mRNA splicing requires cooperation between two 3' splice sites.

We have studied splicing of the polyoma virus early region pre-mRNA in vitro. This RNA is alternatively spliced in vivo to produce mRNA encoding the large, middle-sized (MTAg), and small (StAg) tumor antigens. Our primary interest was to learn how the 48-nucleotide StAg intron is excised, because the length of this intron is significantly less than the apparent minimum established for mammalian introns. Although the products of all three splices are detected in vitro, characterization of the pathway and sequence requirements of StAg splicing suggests that splicing factors interact with the precursor RNA in an unexpected way to catalyze removal of this intron. Specifically, StAg splicing uses either of two lariat branch points, one of which is located only 4 nucleotides from the 3' splice site. Furthermore, the StAg splice absolutely requires that the alternative MTAg 3' splice site, located 14 nucleotides downstream of the StAg 3' splice site, be intact. Insertion mutations that increase or decrease the quality of the MTAg pyrimidine stretch enhance or repress StAg as well as MTAg splicing, and a single-base change in the MTAg AG splice acceptor totally blocks both splices. These results demonstrate the ability of two 3' splice sites to cooperate with each other to bring about removal of a single intron.

Antigens, Polyomavirus Transforming↗

Non-suture repair of tendons.

A set of metal clamps cooled by liquid nitrogen were developed to hold the ends of tendons during in vitro tension tests. These were then used to determine the strength of whole human tendons, and human tendons repaired in vitro by sutures or by cyanoacrylate glues with or without suturing. The mean ultimate load of the intact human tendons was 1065 N, and the ultimate stress was 99.02 MN m-2. The repairs gave values of 0.3-2.3% of those of intact human tendon. Tendons were then repaired in vivo by a Kessler suture technique using braided polyester sutures with or without augmentation by a butyl cyanoacrylate glue (Histoacryl). At six months post-repair all tendons were as strong as the unoperated contralateral tendons. The glue augmented sutures were stronger than those not so augmented. A scanning electron micrograph of a tension tested glued tendon showed that it had failed by cohesive failure, i.e. failure of the inter-glue bonds rather than failure at the glue/tendon interface.

Animals↗

Biomechanical assessment of the effects of significant hamstring injury: an isokinetic study.

Soccer players may develop recurrent hamstring injuries. This may be due to inadequate rehabilitation or to recurrent injury. In addition, following injury, the hamstring muscular complex may be permanently damaged, resulting in decreased strength, and increased likelihood of recurrent injury. Fourteen professional soccer players were assessed by clinical examination and by isokinetic testing with a Cybex II machine. Seven had suffered moderate or major hamstring injuries in the past year. There were seven controls. None of the hamstring group were currently suffering from an acute hamstring injury. The results of the two groups were compared. There were no differences in the mean results. This pilot study suggests that no permanent functional damage occurs to the muscular complex after moderate or major hamstring injuries after correct treatment. However, further research is required to confirm this.

Adolescent↗

An evaluation of suture materials used in tendon surgery.

The following suture materials have been evaluated for their suitability for use in flexor tendon repairs: 4/0 gauge monofilament and multifilament stainless steel, mono-filament nylon, monofilament polypropylene, monofilament polybutestor, braided polyester, braided polyglycolic acid and a monofilament polyglyconate. These were investigated for their tensile strength (both knotted and unknotted), their extension to failure and knot-holding properties. Stainless steel and monofilament polyglyconate appeared to be the most suitable in that they had high tensile strengths, both knotted and unknotted, and had good knot-holding security. The only disadvantages are that stainless steel is difficult to use and monofilament polyglyconate is absorbable. Polypropylene and braided polyester, although having lower tensile strengths, are reasonable alternatives.

Humans↗