Search PubMed⌕ Search

Biomedical subjects

J Niemi

Publications and source records attributed to J Niemi.

25 records · Page 2Linked to original sources
PubMed1986

A new approach to high sensitivity differential hybridization.

We describe a new approach to differential hybridization, designed to identify cDNA clones representing rare mRNA species. Duplicate filters carrying a library of cDNA from phorbolmyristate acetate (PMA)-induced EL-4 cells in lambda gt11 were hybridized with high concentrations of unlabeled, cloned, single-stranded cDNA from induced and control EL-4 cells, respectively. Plaques binding single-stranded cDNA were revealed by a second round of hybridization with 35S-labeled DNA complementary to the vector moiety of the single-stranded cDNA. Plaques corresponding to PMA-induced mRNAs occurring at a level of about 1 part in 15,000 were isolated. We believe the method is at least ten times more sensitive than conventional differential hybridization.

Animals↗
PubMed1985

Phoneme errors in Broca's aphasia: three Finnish cases.

The phonemic errors of three Finnish Broca's aphasics were analyzed according to various phonological features, frequency of segments, syllable, word, and stress positions, as well as for the phonotactic structure of the uncanonical products. It was found, e.g., that the distance between the syntagmatic error and its source could not be satisfactorily counted by phonemes, since the syllable had to be taken into account also in pathological Finnish. As regards the paradigmatic dimension of the errors, the number of incorrect features is even here in reverse relation to the frequency of the erroneous phonemes. Moreover, it was found that pure quantity errors and vowel harmony deviations are infrequent in the speech of Finnish Broca's aphasics.

Adult↗
PubMedDate not supplied

Linearity and morphological structure in derived words: evidence from category decision.

The present article investigates the influences of word-internal category information and linearity on the processing of morphologically complex words. Three sets of Finnish derived nouns consisting of a noun, adjective or verb stem, and a suffix were submitted to two on-line category decision tasks, where the subjects had to decide whether the word was a noun or a verb respectively. Both experiments show a stem-suffix category mismatch effect manifesting itself in prolonged reaction time latencies. Thus, the results indicate that the order of presentation and the word-internal structural information are intimately tied together also in the visual processing of morphological complex words.

Cognition↗
PubMedDate not supplied

Finnish compound structure: experiments with a morphologically impaired patient.

The present study addresses two major aspects of compound processing, namely the issues of access code and structural effects (headedness), with an extensively studied deep dyslexic patient, HH, who has previously been shown to suffer from morphological impairment. In oral reading of compounds, HH preserves their morphological structure and, at the same time, shows additional processing load in increased errors compared to monomorphemic and derived words. Our data suggest that semantically transparent Finnish compounds are decomposed into their constituents at the level of lexical access. Furthermore, there is a processing asymmetry between the constituents, since several qualitative features of HH's reading performance suggest that it is the first constituent which primarily serves as the initial access code.

Dyslexia↗
PubMedDate not supplied

Morphological isolates in idioms: cranberries or real words?

The present study focuses on a subtype of Finnish nouns that appear only as complements in idiomatic verb phrases. In addition to the idioms as their sole environment, these idiomatic isolates, as we call them, are typically frozen to a single case form. In two experiments, in a subjective rating task and a lexical decision task, the isolates are pitted against ordinary nouns and nouns that appear as frozen forms in idioms in addition to being ordinary, free words. The experiments show that the isolates, in spite of their defective syntactic and morphological properties, are processed like ordinary lexical items.

Cognition↗
PubMedDate not supplied

Production of grammatical number in specific language impairment: An elicitation experiment on Finnish.

A wug type of plural production task with Finnish-speaking 10- to 11-year-old normals and 10-year-old SLI children shows that formal transparency of affixation and the lexicality (real words vs pseudowords) of the stimuli affect the performance of both groups. Not unexpectedly, nontransparent pseudowords receive drastically low correctness rates among the SLI speakers. The failures of the SLI group corroborate the claim that they have not internalized the productive inflectional morphology involved in plural formation to the extent that their normal, age-matched peers have.

Child↗