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Biomedical subjects

J N Miller

Publications and source records attributed to J N Miller.

At least 145 records · Page 8Linked to original sources
PubMed1975

Thin-layer gel chromatography of dyed proteins.

Plasma chromatography as a method for ultratrace qualitative and quantitative detection of organic compounds is especially well suited for detection of gas chromatographic effluents. The optimum range of sample quantity is 10(-6) to 10(-12) g for detection and identification of a compound by use of its characteristic positive and negative mobility spectra. The type of reference mobility spectra produced by alkanes, aromatics, esters, halogenated compounds, nitrogenated compounds and organic acids have been previously reported. This study presents the reference mobility spectra produced for lysergic acid diethylamide (LSD), delta9-tetrahydrocannabinol (delta9-THC), digitoxigenin and several biochemical compounds of research significance. LSD and delta9-THC in a mixture can be detected and identified by plasma chromatography positive mobility spectra in quantities of 10(-7) g or less. All the compounds investigated in this study display strong MH+ ions along with other ions primarily of the type (M)NO+, (M)2H+. None of these compounds exhibits negative mobility spectra.

Chromatography, Gel↗
PubMed1975

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.

The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum.

Animals↗
PubMed1975

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.

The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum.

Anti-Bacterial Agents↗