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J Mustonen

Publications and source records attributed to J Mustonen.

At least 127 records · Page 7Linked to original sources
PubMed1985

Clinicopathologic correlations in a series of 143 patients with IgA glomerulonephritis.

In an unselected series of patients with IgA glomerulonephritis, old age, high blood pressure, and high urinary protein excretion at the time of renal biopsy were found to correlate with impaired renal function, whereas sex, estimated duration of the disease, or high serum IgA levels did not. The following clinical features were favorable prognostic signs: asymptomatic proteinuria, macroscopic hematuria, and isolated microscopic hematuria. The degree of diffuse mesangial alteration and the presence of segmental glomerular lesions correlated clearly with the subsequent clinical outcome. Vascular lesions, i.e. arteriosclerosis and renal vascular deposition of C3, were most often present in patients with severe glomerulopathy. The presence of electron-dense deposits in glomerular capillary walls was also an unfavorable prognostic finding. Renal biopsy findings of interstitial infiltrates of inflammatory cells and IgA distributed along glomerular capillary walls were usually associated with extrarenal manifestations of the disease.

Adolescent↗
PubMed1984

Methodological variation in systolic time intervals.

Systolic time intervals (STI) were recorded six times in 12 male students. All 72 recordings were measured twice by a physician and once by two nurses. By comparing these measurements an intra- and interobserver (= methodological) variation was obtained. For measured parameters (QS2- interval and LVET) variation was less than 2%. For calculated parameters the variation was larger, but did not reach the level of statistical significance. Methodological variation was smaller than physiologic variation. The internal consistency was almost perfect for measured parameters and only slightly worse for calculated parameters.

Adult↗
PubMed1984

Immunoelectron microscopic localization of immune deposits in IgA glomerulonephritis.

Renal biopsy specimens obtained from twelve patients with IgA glomerulonephritis (IgA GN) were studied by immunoelectron microscopy (IEM) concomitantly with light microscopy (LM), immunofluorescence microscopy (IF), and electron microscopy (EM). For IEM, we used horseradish peroxidase (HRP)-conjugated antisera to human immunoglobulins (Ig) and to the complement component C3, and a diffusion technique with periodate-lysine-paraformaldehyde (PLP)-fixed tissue-chopper or cryostat sections. Due to well-preserved ultrastructure and good penetration of the antisera in the tissue-chopper sections, a detailed analysis of the distribution of immune material in the glomeruli was possible. In cryostat sections, ice crystal artifacts could not be avoided. The typical features of IgA GN could be reliably confirmed by IEM. Furthermore, IEM revealed the presence of Ig's and C3 in mesangial channels, in the intracellular vacuoles of glomerular cells, and in the electron-lucent areas along the glomerular basement membranes (GBMs). Staining of the mesangial channels indicates that they represent a route for the immune material gaining access into the mesangium. Intracellular vacuoles suggest that the deposited immune material can be partly eliminated through endocytosis by glomerular cells. The presence of Ig's and C3 in the electron-lucent areas supplies an explanation to the discrepancy sometimes observed between a positive finding in IF and a lack of deposits in EM.

Basement Membrane↗
PubMed1984

Renal biopsy in acute renal failure.

Renal biopsies were performed on 91 out of 99 patients with acute intrinsic renal failure. In all these 91 cases a clinical diagnosis was suggested by an experienced nephrologist unaware of the renal biopsy findings. Most diagnoses agreed with the histopathological findings, but there were important exceptions. On clinical grounds, acute tubulointerstitial renal disease was diagnosed correctly in 77% and acute glomerulonephritis in 56%. In 15% of the patients the clinical data did not permit any certain diagnostic suggestion. In about 20% the biopsy finding definitely influenced the choice of medical treatment. In those 15 patients who needed dialysis treatment, the biopsy findings helped in determining whether this need was temporary or permanent. Our results show that renal biopsy is valuable in settling the diagnosis, in determining the prognosis, and in planning the treatment of acute intrinsic renal failure.

Acute Kidney Injury↗
PubMed1984

IgA glomerulonephritis and associated diseases.

In a series of 184 patients with IgA glomerulonephritis (IgA GN) 114 (62%) had concomitant diseases compared with 35 (36%) in a control series consisting of 96 patients with mesangial glomerulonephritis of other type than IgA GN. This difference was statistically significant. Many of the diseases found in association with IgA GN were immunologic or systemic in nature, affected mucous membranes of the respiratory or gastrointestinal tract or were such as known to be associated with high serum IgA level. These clinical findings supported the idea that IgA GN is often a manifestation of various systemic disease processes.

Adolescent↗
PubMed1983

Immunofluorescence microscopy of paraffin-embedded human kidney specimens obtained by fine-needle aspiration biopsy.

Recently, we have introduced an atraumatic fine-needle aspiration biopsy method to obtain human glomeruli for morphologic investigation. In the present study, immunofluorescence microscopy of paraffin-embedded, fine-needle specimens is described. The specimens were obtained by aspiration with a 10-mL syringe fitted to the fine-needle prepared from a lumbar puncture needle (Jintan Terumo). Embedding of the specimens into conventional paraffin blocks was carried out after pelleting them by centrifugation between processing steps in conical centrifuge tubes. Sections from the blocks were collected on small pieces of GelBond film (FMC Corporation) instead of objective slides, which prevented the detachment of small sections during enzyme treatment. Localization then was performed on deparaffinized trypsin-digested sections using fluorescein-labeled antibodies. The choice of fixative and digestive enzymes was found to have a marked effect on the localization; periodate-lysine-paraformaldehyde fixative and trypsin digestion gave the most reliable results.

Biopsy, Needle↗
PubMed1983

[Kidney biopsy].

Explore the source record for details and available documents.

Biopsy, Needle↗
PubMed1983

IgA nephropathy associated with celiac disease and dermatitis herpetiformis.

IgA nephropathy was found in three patients with celiac disease; two of them also had dermatitis herpetiformis. These observed associations widen the spectrum of the diseases that have been reported to coexist with IgA nephropathy. This study focused on the possible intestinal origin of the glomerulonephritis.

Adult↗
PubMed1983

The nephrotic syndrome in IgA glomerulonephritis: response to corticosteroid therapy.

The nephrotic syndrome was observed in eight out of 170 patients with IgA glomerulonephritis (5%). Three patients had mild glomerular alterations, all of them were normotensive, had normal renal function and responded to treatment with corticosteroids. In five patients moderate to marked mesangial changes associated with segmental sclerosing or proliferative lesions were seen. These patients were hypertensive and four of them had renal insufficiency. Three were treated with corticosteroids without response.

Adrenal Cortex Hormones↗
PubMed1983

Acute rheumatic fever with extracapillary glomerulonephritis and the nephrotic syndrome.

A 34 year old woman is described in whom carditis, arthritis, fever, leukocytosis and a high sedimentation rate developed two weeks after a streptococcal infection. The patient also had the nephrotic syndrome and rapidly progressive renal insufficiency. The renal biopsy specimen showed acute extracapillary (crescentic) glomerulonephritis. The initial response to corticosteroid therapy was good, but later a slowly progressive renal function impairment was seen.

Acute Disease↗
PubMed1982

IgM-associated glomerulonephritis.

In a series of 374 renal biopsy specimens, 26 from patients with primary glomerulonephritis showed IgM as the main glomerular immunofluorescence finding. By light microscopy 17 of these specimens showed mild mesangial hypercellularity and 9 were normal. Small mesangial electron-dense deposits were seen in 8 specimens. Of the 26 patients, 10 had proteinuria, 6 had the nephrotic syndrome, 6 had hematuria and 4 had both proteinuria and hematuria. The mean serum IgM level was significantly higher in the patients than in healthy controls. Circulating immune complexes were detected by three methods in 39% of the patients.

Adolescent↗
PubMed1981

Circulating immune complexes, the concentration of serum IgA and the distribution of HLA antigens in IgA nephropathy.

A series of 40 IgA nephropathy patients is presented. IgA nephropathy was the most common (24%) type of glomerulonephritis in our renal biopsy material. Circulating immune complexes were measured by five methods that gave a positive result in 9-69% of the patients. The platelet aggregation test with heated serum (PAT2) was by far the most commonly positive. The serum concentrations of IgA were measured by two methods, radial immunodiffusion and laser nephelometry. Both tests gave similar results. 58% of the patients had elevated serum IgA. There was no statistically significant association between HLA antigens and IgA nephropathy as compared with the control material.

Antigen-Antibody Complex↗