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Biomedical subjects

J Munro

Publications and source records attributed to J Munro.

At least 109 records · Page 6Linked to original sources
PubMed1973

Immunological studies on the mechanism of gold hypersensitivity reactions.

Immunological studies were performed on 12 patients with rheumatoid arthritis who developed reactions to gold. IgE levels were found to be raised in 10 of 11 patients tested at the time of the gold reaction, returning to normal on stopping therapy. Two of 12 patients with gold reactions had positive in-vitro lymphocyte transformation responses to gold.It is suggested that dermatological side effects in particular are mediated by a type I hypersensitivity response.

Arthritis, Rheumatoid↗
PubMed1969

Medicare.

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Insurance, Health↗
PubMedDate not supplied

[Development of a synthetic positive control which also detects plasmid contamination in diagnostic polymerase chain reaction].

This paper describes a technique for the development of a positive control for use in a nested PCR to show that the PCR has worked correctly with both outer and inner primers designed for diagnostic amplification of 618 bp and 317 bp products respectively. This positive control produces a larger product than the diagnostic sample that can be discriminated on an agarose gel. This technique is advantageous over traditional cloning of the diagnostic PCR product itself by: 1) making it visually easy to detect plasmid contamination and thus, prevent false positives from the plasmid; 2) develop a positive control when the target organism is at a very low prevalence so initial detection is not relied on for cloning positive controls. This will ensure the PCR is working correctly prior to diagnostic sampling, reducing false negatives; or 3) for developing a PCR and determining the sensitivity prior to the use of diagnostic samples. The methods used to produce this nested positive control demonstrates how to use large oligonucleotide primers in PCR without non-specific binding occurring.

DNA Primers↗