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Biomedical subjects

J Mukoyama

Publications and source records attributed to J Mukoyama.

5 recordsLinked to original sources
PubMed1989

Detection of anti-rotavirus IgG, IgM, and IgA antibodies in healthy subjects, rotavirus infections, and immunodeficiencies by immunoblotting.

Immune responses to individual simian rotavirus (SA 11) structural polypeptides were studied with emphasis on specific IgG, IgM, and IgA in paired sera from four children with rotavirus infections. Responses to simian rotavirus (SA 11) were also studied in 103 healthy children, 10 patients with primary immunodeficiency who received intravenous immunoglobulin, and 11 human immunodeficiency virus antibody-positive patients. All samples were immunoblotted for two major polypeptides--VP2 and VP6--of IgG. Immunoblotted IgA and IgM were elevated 2-4 weeks after the onset of the disease (stage II) in patients with rotavirus infection. Immunoblotted IgG remained almost the same at the onset of the disease and stage II. The scores for primary and secondary immunodeficiency patients were lower than for healthy children. The score obtained by totaling all polypeptides found in each individual was compared with the optical density (OD) value obtained by enzyme immunoassay.

Acquired Immunodeficiency Syndrome↗
PubMed1978

Acute gastroenteritis among schoolchildren associated with reovirus-like agent.

During early summer 1975 and spring 1976, outbreaks of acute gastroenteritis were reported from primary schools and other institutions in several districts of Japan. Outbreaks occurred in an explosive manner resembling mass food poisoning from a school lunch. The majority of patients were in the age group 6-14 years. Clinical features were generally mild, consisting of vomiting and/or diarrhea, often with low-grade fever. Reovirus-like agents in the feces were found in 27 (44%) of 62 patients. The virus found in feces of schoolchildren with acute gastroenteritis (SCGV) was related morphologically as well as serologically, not only to the agent found in infantile gastroenteritis (IGV), but also to neonatal calf diarrhea virus (NCDV). A slight difference in antigenicity between SCGV and IGV as suggested by cross complement fixation (CF) remains to be elucidated. About one-half of paired sera from 54 patients showed a significant rise in CF antibody against SCGV and/or NCDV. The pattern of neutralizing (NT) antibody against NCDV in patients' sera was similar to that of CF antibody. Most children studied had a titer of 1:4 or greater of CF and/or NT antibodies to SCGV and NCDV in acute sera. The relationship between acute gastroenteritis associated with reovirus-like agent in infants and that in schoolchildren is discussed.

Acute Disease↗
PubMed1977

Isolation and characterization of a parvovirus of rabbits.

The isolation and characterization of a new virus from rabbit stool are described. The virus replicated in rabbit kidney cell cultures and agglutinated human group O erythrocytes at 4 degrees C. It was stable at acid pH and resistant to chloroform and heat treatment. The growth of the virus was inhibited by 5-iodo-2-deoxyuridine, and virions were stained red with acridine orange, suggesting that they contain single-stranded deoxyribonucleic acid. The density of virions was 1.41 to 1.44 g/ml in CsCl, and the sedimentation value was 137S in sucrose at 4 degrees C. The infectious particles had cubic symmetry and were 27 to 28 nm in diameter by electron microscopy. By these properties this virus can be classified as a member of the parvovirus group. Antibody response was demonstrated in the rabbit from which this virus was recovered. A number of rabbits from a commercial source were found to contain hemagglutination-inhibiting antibody to this virus.

Acridines↗