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Biomedical subjects

J Mora-Galindo

Publications and source records attributed to J Mora-Galindo.

24 records · Page 2Linked to original sources
PubMedDate not supplied

[In vitro model for the analysis of the interaction between Shigella flexneri and the intestinal epithelium].

An in vitro study of the adhesion and invasion of Shigella flexneri was implemented, by means of incubation of laminary cuts of cecal mucosa of Dunkin-Hartley guinea pigs in a suspension of Shigella flexneri, which was isolated from a patient with bacillary dysentery. The laminae were placed in plastic chambers for two hours at 37%C. After this the bacterial suspension was discarded so as to eliminate the bacilli which were not adhered. The epithelium was washed with saline and was processed for analysis with scanning electron microscope. The topology of the mucosa incubated with Shigella flexneri was similar to that of the witnesses. The bacteria which adhered to the mucosa were dispersed individually or in clumps of varied numbers. The main alteration observed upon the epithelial surface were depressions due to a lateral separation of the microvilli which may have originated the endocytic stomas containing bacterias. The results of this study allow the proposition of the use of explants, so as to study the interaction between Shigella flexneri and the intestinal epithelium, with the possibility of modifying different experimental variables.

Animals↗
PubMedDate not supplied

Elimination of epimural microflora from guinea pig cecal epithelium by antimicrobial drugs.

The effect of antimicrobial drugs on epimural bacteria of guinea pig cecum was assessed by scanning electron microscopy. Metronidazole (500 hg/ml), neomycin (50 hg/ml) or both combined were given orally in drinking water ad libitum for five consecutive days, and representative fragments of cecum were processed for analysis. In untreated animals large areas of mucosa were lined by spiral-shaped bacteria were eliminated by treatment with neomycin, only remaining fusiform bacteria at openings of crypts. metronidazole was effective for eliminating all bacterial populations; the same effect was achieved with a combination of neomycin and metronidazole, rendering the cecum free of epimural bacteria, the health of guinea pig was unaffected by these treatments. The cecal epithelium of antimicrobial-treated animals can be used for experimental studies without interference of epimural bacteria.

Administration, Oral↗
PubMedDate not supplied

[Massive production of Entamoeba histolytica cysts under axenic conditions].

After an exponential phase of growth, HK9 strain amebas, kept in the axenic medium PEHPS, spontaneously acquire a form morphologically similar to various natural cysts, as well as a resistance to hypotonic shock, due to the effect of a wall, partially composed of polysaccharides. The number of differentiated amebas increases gradually, although their viability diminishes, in function of the incubation time. On the ninth day, 96% of the population is made up of these cells, although only 6% are viable. The ultramicroscopic structure of the great majority of differentiated amebas corresponds to that of immature cysts. These, and the PEHPS medium, constitute a good model for a characterization of the start of the differentiation of E. histolytica, and open the opportunity of obtaining in axenic conditions, massive cultures of mature cysts.

Animals↗