Search PubMed⌕ Search

Biomedical subjects

J Molenda

Publications and source records attributed to J Molenda.

At least 19 recordsLinked to original sources
PubMed1994

Rabies enzootic situation in animals on Lower Silesia in 1983-1992.

Considerable increase (average 70%) of rabies cases in animals on Lower Silesia, occurred in second half of the decade 1983-1992. Foxes were a reservoir of the virus and epizootic spread of the disease had been noted among them. In other animal species, both wildlife and domestic, only sporadic outbreaks were noted. They were a consequence of contact of these animals with infected foxes, in which from 85% to 90% incidences a year were stated. Parallel rise of rabies cases in cats makes this animal the most important potential vector of the disease to man.

Animals↗
PubMed1990

Sensitivity of canine Staphylococcus intermedius strains to the phages typing Staphylococcus aureus isolated from man.

98 staphylococci strains isolated from skin lesions of dogs were tested for their morphological, physiological and biochemical characteristics. These examinations have proved that the majority of the strains was Staphylococcus intermedius (n = 97), and only one was Staphylococcus aureus. The strains were also tested for their sensitivity to bacteriophages. Most of Staphylococcus intermedius strains (n = 75) could be typed with the set of human phages, typing staphylococci isolated from man. Two phages, 676 and Phi 131 have turned out to be highly active towards a large number of the isolates.

Animals↗
PubMed1990

[Haemophilus somnus--properties and isolation].

An account is given in this paper of characteristic parameters of Haemophilus somnus, the aetiological factor of various cattle diseases. Morphological properties of the pathogen are described together with techniques for isolation and identification. Also discussed are its physiological, biochemical, and antigenic properties as well as its present position in taxonomy and the structure of the outer membrane.

Animals↗
PubMed1985

Relapse of host leukemic lymphoblasts following engraftment by an HLA-mismatched marrow transplant: mechanisms of escape from the "graft versus leukemia" effect.

Leukemic relapses and graft versus host disease (GvHD) remain major complications following allogeneic bone marrow transplantation for leukemia. We present clinical and laboratory details for an eight-year-old boy who received a T-cell-depleted HLA-mismatched marrow transplant as therapy for acute lymphoblastic leukemia (ALL) in second remission. Engraftment with donor marrow was prompt and without any acute GvHD. Nevertheless, the patient's original ALL recurred and proved fatal. The patient remained a chimera with persistent donor lymphocytes present at the time of posttransplant relapse and subsequent to treatment with unsuccessful reinduction chemotherapy. In vitro immune studies showed that these leukemic cells could be recognized and destroyed by the donor's lymphocytes. The relapse itself suggests, however, that the donor's lymphocytes did not effectively destroy the patient's histoincompatible ALL cells in vivo following establishment of the chimeric state. Potential mechanisms are presented to account for this presumed "escape" from the postulated "graft versus leukemia" effect.

Acute Disease↗
PubMed1985

HLA typing and karyotyping of limited numbers of lymphocytes is facilitated by in vitro expansion with TCGF.

It is often difficult to obtain sufficient quantities of leukocytes from bone marrow (BM) transplant recipients for post-transplant immunological monitoring and evaluation of engraftment. We have developed a cell culture expansion technique that allows HLA-A,B, and DR typing as well as karyotyping on limited numbers (less than 10(7)) of lymphocytes. Lymphocytes from healthy control donors were allo-activated in vitro and further expanded by culturing in T-cell growth factor (TCGF). After 14 days of culture, these cells were sent for blind HLA-A,B and DR typing and for karyotyping. Surface marker studies showed that 90% of the T-cells expanded in growth factor are OK-la positive. HLA typing with these DR-bearing in vitro expanded T-cells showed excellent correlation with HLA typing on fresh unexpanded lymphocytes. Karyotyping of expanded T-cells showed normal male or female cells as expected. This technique may be especially valuable when testing pediatric or adult patients with very low lymphocyte counts.

Bone Marrow Transplantation↗
PubMed1983

Influence of immunisation with enteropathogenic and non-enteropathogenic Escherichia coli on the occurrence of loop dilation in rabbits.

The protective effects of high levels of O115 antibodies and K88 antibodies in the sera of hyperimmunized rabbits on the intestinal loop dilation elicited by live cultures of Escherichia coli O115: K88:H39 and its enterotoxins were examined. K88 antibodies did not prevent fluid accumulation when live E coli O115:K88:H39 or its enterotoxins were injected into ligated loops in rabbits actively immunised with K12:K88 or when K88 antiserum was mixed directly with the challenge shortly before injection into the loops. In two of six rabbits immunised with heat-killed O115 E coli the live strain failed to elicit a fluid response and in two others the response was reduced. In all cases the O115 antiserum inhibited the fluid response evoked by the live, homologous bacteria, but not by enterotoxin preparations, when the serum was mixed directly with the challenge before injection.

Animals↗
PubMed1982

[Behavior of antibodies against Enterobacteriaceae common antigen in the serum and colostrum of sows immunized with Escherichia coli].

Specific anti-O E. coli and anti-CA (common antigen of Enterobacteriaceae) hemagglutinins were titrated in sera and colostrum of sows immunized against colibacillosis. Pregnant sows had been immunized with vaccines prepared in different ways on Escherichia coli 0115:K88 (pathogenetic strain in the environment of the farm) for 2-3 weeks before parturition. The vaccines were given orally--the suspension of living bacteria; intramuscularly--formaline killed E. coli 0115:K88 or extract obtained by treating the bacteria mass with EDTA buffer. Samples of sera and colostrum were taken 24 hours after parturition and were titrated by means of the passive hemagglutination test performed on plastic trays. With the increase of specific anti-O antibody, a simultaneous rise of anti CA antibody level was observed.

Animals↗
PubMed1981

[CA antibodies (Enterobacteriaceae common antigen) in the sera of domestic animals].

Using the indirect hemagglutination test, antibodies against Enterobacteriaceae common antigen (CA) were tested in the sera of 123 horses, 142 cows, 108 sheep, 142 mature pigs and 60 piglets (3-4 weeks of age). Anti CA antibody level and antibody titers for somatic antigens (phenol-water extracts) various serogroups of E. coli (0149, 0138, 0115, 078, 09) and S. typhimurium were compared. Ca antibodies in titer equal or higher than 1:15 were found to occur in 100% of the examined horses and cows, while in the sera of 92% sheep, 80% of mature pigs and 60% of piglets antibodies to the common Enterobacterial antigen were present in titer equal or higher than 1:7.5. In all sera examined the antibody level to somatic antigens of E. coli and S. typhimurium exceeded that to Enterobacteriaceae common antigen. The influence of absorption of sera with CA preparation on the antibody level to heterologous antigens was also examined. It was found that the absorption caused a statistically significant decrease of the titers to O antigens E. coli and S. typhimurium in comparison with those found in unabsorbed sera.

Animals↗
PubMed1980

[The protective effect of an EDTA-sodium-extract-vaccine obtained from swine-pathogenic E. coli in a field trial. 1. Communication: Direct and indirect immunization of suckling piglets (author's transl)].

Pentavalent EDTA-Sodium-extract-vaccines and monovalent EDTA-Sodium-extract-vaccines obtained from swine-pathogenic E. coli and produced in Berlin-Buch were used in field trials to test their antiepizootic effect in industrial pig-farms in the district of Wrocław. The pentavalent vaccine contained extracts from E. coli O138, O139, O141, O147, and O149, whereas extracts from O8, O115 and O149 were used as monovalent vaccines. The field trials were conducted as blind trials is closed stocks of pigs. We employed direct oral immunization of suckling piglets and indirect immunization via sow vaccination. Direct oral immunization was tested in 382 suckling piglets. 190 animals were vaccinated, 192 animals received placebo. Neither the pentavalent vaccine nor a monovalent vaccine obtained from an epizootie strain of this stock gave protection in terms of a decrease in the duration of disease or a reduction of morbidity and mortality. Direct oral-immunization of suckling piglets thus was ineffective. Indirect immunization of the suckling piglets via vaccination of sow was performed in 189 pregnant sows 18--15 days before farrowing. 84 sows received vaccines i.m., 105 were given placebo i.m. These sows farrowed 1717 pigs, 777 of which were immunized indirectly and 940 treated with placebos. With the pentavalent vaccine and with the monovalent vaccine obtained from an epizootic strain we achieved significant protection of the indirect vaccinated suckling piglets.

Administration, Oral↗
PubMed1980

[The protective effect of an EDTA-sodium-extract-vaccine obtained from swine-pathogenic E. coli in a field trial. 2. Communication: Immunization of weanling piglets (author's transl)].

Pentavalent EDTA-Sodium-extract vaccines and monovalent EDTA-Sodium-extract vaccines obtained from swine-pathogenic E. coli were used to test their antiepizootic effect in industrial pig farms, 2929 piglets after weaning were included in this field trial, 1309 were immunized partly orally, partly i.m., 1620 piglets received placebos accordingly. By means of oral immunization the pentavalent vaccine was capable of reducing the morbidity of the vaccinated animals significantly. Intramuscular immunization with the pentavalent vaccines produced significant differences in morbidity too. A monovalent vaccine obtained from E. coli O149 and E. coli O8 was shown to be equally effective, whereas a monovalent vaccine obtained from E. coli O115 was ineffective. But in this stock there were infections with O149 but none with O115 or O8.

Administration, Oral↗
PubMed1979

[Preliminary results of an atiepizootic field test using EDTA-Na extract vaccine from Escherichia coli strains pathogenic to swine in swine production industrial units].

An anti-epizootic field test was applied to industrialised pig farms in the region of Wrocław, Poland, to test the effectiveness of a pentavalent EDTA (calcium disodium edetate)--sodium vaccine extracted from Escherichia coli strains with pathogenicity to swine. The vaccine had been received from a centre in Berlin-Buch, GDR. The vaccine failed to provide any protection, when orally applied to nursed piglets. However, both morbidity and mortality were reduced and, thus, an anti-epizootic effect on nursed piglets produced, when the vaccine had been injected intramuscularly to the pregnant mother animals, prior to farrowing. In weaned piglets morbidity was sucessfully reduced by both oral as well as intramuscular administration.

Administration, Oral↗
PubMed1975

[Course of beta-hemolytic E. coli carrier state in piglets during weaning].

The investigations were carried out on 22 sows and 44 piglets (one sow and 2 piglets from each litter). The pigs were divided into 4 groups according to the different additions to their diet during weaning which might have an effect on the carrier state and dissemination of beta-haemolytic E. coli. Group I was given a feed concentrate and separated milk, group II - a feed concentrate and sour milk, group III - a feed concentrate and milk with addition of garlic (200 mg/kg of body weight), group IV - a feed concentrate and milk with addition of vitamin C (20 mg/kg of body weight). The beta-haemolytic E. coli carrier state was examined before starting supplemental feeding (3 times between the 21st and 30th day of life) and after weaning (3 times between the 49th and 56th day of life). The carrier state under discussion was found in 27.2% of sows and in 25% of piglets at the beginning of supplemental feeding, and in 40.1% of sows and 36.3% of piglets during weaning, which means an increase of the number of carriers both among the sows and piglets at that time. Twenty-two strains of E. coli isolated from the carriers belonged to serotypes responsible for colibacteriosis of pigs (O149:K91, K88a, c, O8:K87, K88a, c, O139:K82); 23 strains belonging to E. coli groups O6, O8 and O116 were devoid of K and H antigens, 9 strains possessed a double O antigen (O9, O60: :H-, O117, O120: :H-, O102, O25: :H21). An inhibitory effect of the addition of sour milk to the feed on the intensity of the carrier state of beta-haemolytic E. coli was observed in piglets kept on that diet. The pathogenic strains of E. coli were observed more often in the carrier-piglets (60%), and in the sows predominated the strains of beta-haemolytic E. coli devoid of the K antigens.

Animals↗