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J Minowada

Publications and source records attributed to J Minowada.

355 records · Page 20Linked to original sources
PubMed1967

Specific transplantation antigens of mouse sarcomas induced by adenovirus type 12.

A specific isograft resistance against three different mouse adeno 12 sarcomas was demonstrated in mice treated with four to eight doses of viable or X-ray-killed adeno 12 mouse tumors. Whole-body X-ray irradiation with 350 R 24 hr previous to the test challenge did not abolish the resistance, indicating that it was due to a true anamnestic immune reaction. This was further proven by the finding that similar treatment with tumors of other origin did not induce any immunity, nor did the treatment with adeno 12 tumor material induce any immunity against two neoplasms of Schmidt-Ruppin-Rous viral origin. The previous report by Trentin et al. (17) that adeno 12 infection leads to a specific transplantation immunity was fully confirmed. When the specificity of this virus-induced immunity was studied it was discovered that besides adeno virus type 12, type 7 and probably type 18 also gave the same type of resistance while adenovirus type 5 did not. A contamination of the adeno 7-infected mice with adeno type 12 was excluded by testing pooled sera from these animals for anti-adeno 12 CF or HI antibodies.

Adenoviridae↗
PubMedDate not supplied

Chromosome studies in stimulated lymphocytes of B-cell chronic lymphocytic leukemias.

Using a sister chromatid differentiation technique, cell cycle study of stimulated lymphocytes of B-cell chronic lymphocytic leukemia (B-CLL) revealed their cell cycle progression to be similar to that of normal lymphocytes stimulated by T-cell and various polyclonal B-cell activators (PBA). The chromosome constitutions of stimulated lymphocytes in 62 patients with B-CLL were examined using PBA such as Epstein-Barr virus (EBV) and lipopolysaccharide W from E. coli 055:B5 (LPS). Of the 20 patients with abnormal clones, 11 patients had trisomy 12; other less common abnormalities were trisomy 1, 6q-, i(7q), 14q+, trisomy 16, trisomy 18, reciprocal translocations, and marker chromosomes of unknown origin. These findings indicate that trisomy 12 may be a unique and common karyotypic change in B-CLL. The fact that 3 out of 4 patients with marker chromosomes showed stage IV disease may indicate that a clone with a marker is a predictor of an unfavourable prognosis. The near correlation between trisomy 12 and kappa chains existed (0.05 less than p less than 0.10). Trisomy 12 was seen in all 5 patients with monoclonal paraprotein.

Aged↗
PubMedDate not supplied

Erythroleukemia cell lines HEL and K-562: changes in isoenzyme profiles and morphology during induction of differentiation.

The effects of the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and of hemin on the human erythroleukemia cell lines HEL and K-562 were studied using the parameters cellular morphology, cell proliferation, positivity in the nitro-blue tetrazolium (NBT)-reduction test, adherence to surface, development of pseudopodia, and isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) separated by isoelectric focusing on horizontal polyacrylamide gels. TPA and hemin induced similar morphological changes in HEL and K-562, erythrocyte-like cells were seen to be the predominant population after exposure to hemin. Both TPA and hemin led to cessation of cell proliferation and to a dose-dependent decrease in the number of viable nucleated cells. Whereas HEL cells treated with TPA became positive in the NBT-reduction test, adhered to plastic surface and showed pseudopodia, HEL cells exposed to hemin and K-562 cells cultured with TPA or hemin did not show these markers indicative of the macrophage-cell lineage. TPA-treated HEL cells exhibited isoenzyme patterns which were characteristically seen in cells of the monocyte-macrophage complex. 'Myeloid isoenzyme profiles' were found in TPA-treated K-562 cells. As evidenced by the above described phenotypical changes, HEL cells appeared to differentiate along the macrophage and erythroid cell lineages. Induced K-562 cells displayed myeloid and erythrocytic markers.

Acid Phosphatase↗
PubMedDate not supplied

Induction of differentiation in chronic myelocytic leukaemia cells by the phorbolester TPA.

The mononuclear peripheral blood cells from eight patients with chronic myelocytic leukaemia (CML) were incubated in cell suspension culture in the presence of the phorbolester 12-O-tetradecanoylphorbol 13-acetate (TPA). TPA caused the treated cells to adhere and to acquire morphological and functional features characteristic of macrophage-like cells. Using isoelectric focusing distinct changes in the isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase were detected in the TPA-exposed cells. Besides an increase in the number and staining intensity of isoenzymes of all enzymes, TPA triggered the new expression of a monocyte-specific esterase isoenzyme isoenzyme and of the tartrate-resistant acid phosphatase isoenzyme. The latter two isoenzymes represent further parameters of the monocyte/macrophage complex. The results indicate that immature leukaemic cells arrested along the granulocytic cell axis retain the ability to transform to macrophages.

Cell Count↗
PubMedDate not supplied

Is the Hodgkin cell a T- or B-lymphocyte? Recent evidence from geno- and immunophenotypic analysis and in-vitro cell lines.

The cellular derivation of Hodgkin (H) and Reed-Sternberg (RS) cells remains a controversial issue. A large body of conflicting results in the literature led to a variety of discordant speculations. The application of immunophenotyping, molecular biology and tissue culture provided additional means for investigations on the nature of H-RS cells. Using immunoenzymatic staining with monoclonal antibodies (McAbs) at the single cell level, H-RS cells from many, albeit not all cases, were shown to be positive for T- and/or B-cell markers; none of the cases were labelled by anti-myeloid or anti-monocyte/macrophage McAbs. A common feature of the otherwise heterogeneous immunoprofile is the expression of activation and proliferation antigens besides HLA-DR class II antigen and the X-hapten. Clonal rearrangements of T-cell receptor or immunoglobulin chain genes were found in 22 per cent of cases. The low frequency of positive samples might be due to the commonly low number of H-RS cells in the total population which can lay below the threshold of sensitivity for genotypic analysis. Several cell lines containing H-RS-like cells have been established. These cell lines have geno- and immunophenotypic characteristics of T- or B-cells, but lack properties of myeloid cells or monocytes/macrophages. In the absence of evidence in support of an origin from monocytes/macrophages or other non-lymphoid cells the here reviewed data, which are based on geno- and immunophenotypic analysis of fresh and cultured H-RS cells, provide a lead to a lymphoid derivation of H-RS cells.

B-Lymphocytes↗
PubMedDate not supplied

Morphological, immunophenotypical and isoenzymatic profiles of human leukemia cells and derived T-cell lines.

Six monoclonal leukemia cell lines (MOLT 12-17) were established from three patients with acute leukemia. Two of the patients' samples were of the T-ALL type, the third had morphological, isoenzymatic and immunological features of AMoL. The cell lines resulting from these original cells displayed distinct, but stable marker profiles. A comparison between primary cells and resulting cell lines showed that the cell lines established from patients with T-ALL (MOLT 12, 13, 14 and MOLT 16, 17) expressed similar phenotypes and isoenzyme patterns, but were different in a few specific aspects. The changes suggest a modulation of marker expression in vitro or an arrest at a more immature stage of differentiation than the original cells. The cell line MOLT 15 established from the case of AMoL exhibited a quite discordant profile when compared to the primary sample: the cells lacked all previously found myelomonocytic antigens and a monocyte-specific esterase isoenzyme, but expressed the T-cell associated CD7 antigen and had rearrangements and RNA transcripts of the T-cell receptor beta and gamma chain genes. This striking discrepancy between the patient's malignant cells and the corresponding cell line suggests that subpopulation selection had occurred in culture.

Acute Disease↗
PubMedDate not supplied

Differential platelet-associated factor gene expression by a panel of human cell lines with megakaryoblastic properties after treatment with phorbol myristate acetate.

Four human megakaryocytoid cell lines, namely MOLM-1, MOLM-7, MEG-01 and HEL, were treated with phorbol 12-myristate 13-acetate (PMA) and expression of the platelet-derived growth factor (PDGF) A chain, von Willebrand factor (vWF) and endothelial cell growth factor (ECGF) genes was examined by the reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers. The gene for PDGF A chain is constitutively weakly expressed by MEG-01 cells and strong expression is induced in MEG-01, MOLM-1 and MOLM-7 but not in HEL cells after treatment with PMA for three days. All four cell lines express the vWF gene both constitutively and after exposure to PMA. None of the cell lines constitutively express the gene for ECGF but MEG-01 cells can be induced to do so after treatment with the phorbol diester. Immunohistochemical staining after exposure to PMA showed that the expression of the platelet-associated markers CD41 and CD61 was enhanced in all cell lines indicating possible differentiation along the megakaryocyte lineage. Our results illustrate differential platelet-associated factor gene expression in different megakaryoblastic cell populations in response to treatment with PMA, and suggest that expressions of the PDGF A chain gene and the ECGF gene may be good markers for megakaryocyte maturation.

Base Sequence↗
PubMedDate not supplied

Chromosomal banding of cultured T and B lymphocytes.

Chromosomes of thymus-derived lymphocyte (T-cell) cell lines (CCRF-CEM, CCRF-H-SB-2, MOLT-3, MOLT-4, and RPMI 8402) and the corresponding lines (CCRF-SB and RPMI 8392) with thymus-independent lymphocyte (B-cell) characteristics, were examined with banding techniques. The pairs of cell lines (CCRF-H-SB-2 and CCRF-SB, and RPMI 8402 and RPMI 8392, were confirmed to be of the same origin, respectively, inasmuch as their individual specific variant bands were identical. The MOLT-3 and MOLT-4 cells had common marker chromosomes, indicating that the chromosomal changes in these cells probably occurred in vivo. All of the T-cells had structurally abnormal marker chromosomes, whereas the B-cells were chromsomally normal. The structural changes of the chromosomes suggest that the malignant nature of the present T-cells may be essential for such T-cells to be established in vitro in a standard culture medium.

B-Lymphocytes↗
PubMedDate not supplied

A murine monoclonal antibody (928) recognizing a new epitope formed with a combination of HLA-DPA1*0201 and DPB1*0301 gene products.

A murine monoclonal antibody (mAb), 928, that recognizes a cell surface antigen (928 Ag) on a human Epstein-Barr virus-transformed fetal liver-derived lymphoid progenitor cell line (FL4.4) was generated. The 928 mAb reacted with only FL4.4; it did not react with any other 57 cell lines tested. Two color flowcytometry analysis of peripheral blood mononuclear cells (PBMC) revealed that the 928 mAb reacted with B cell and monocyte fractions from only two individuals out of 63 unrelated donors. Biochemical analyses showed that the 928 Ag composes of two molecules (33 and 34 Kd) and forms a SDS-resistant, noncovalently linked dimer conformation, the feature being similar to that of peptide-bound MHC class II molecules. Treatment of FL4.4 cells with the 928 mAb significantly facilitated homotypic cell aggregation. In addition, treatment of PBMC of the 928 Ag+ donor with recombinant IL-4 augmented the expression of the 928 Ag on CD64+ monocytes. Typing of HLA-DRB1, DPA1 and DPB1 alleles of the 928 Ag expressing and nonexpressing cells revealed that the 928 Ag is expressed only on PBMC of HLA-DPA1*0201 and DPB1*0301 positive donors. Finally, anti-DP antibody precleared 928 Ag from the cell lysate. These results demonstrate that the 928 mAb recognizes a polymorphic determinant of HLA-DPA1*0201-DPB1*0301 gene products. The possibility that amino acids in the groove of the peptide-binding site of HLA-DP molecules are critical for the 928 epitope is discussed.

Animals↗