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J Minowada

Publications and source records attributed to J Minowada.

At least 217 records · Page 12Linked to original sources
PubMed1982

[Interferon production in human T lymphoblastoid cells].

Human T lymphoblastoid cell (RPMI 8402 cell) produced interferon (IFN) through the induction by Sendai virus. The priming effect on the interferon production in the RPMI 8402 cell could be found by the pretreatment of human leukocyte IFN (Hu IFN-alpha), but not by that of the IFN produced in the RPMI 8402 cell (T-IFN). The superinduction by the irradiation of ultraviolet rays or the treatment of antimetabolites (actinomycin D and cycloheximide) or 5-bromodeoxyuridine was not found. The T-IFN was completely neutralized by the anti-Hu IFN-beta serum, but not by the anti-Hu IFN-alpha serum at all. In conclusion, it was confirmed that the IFN produced in the RPMI 8402 cell through the induction by Sendai virus was antigenically identical to Hu IFN-beta.

Antigen-Antibody Complex↗
PubMed1982

Production and characterization of human T leukemic lymphoblastoid cell interferon.

Although human lymphoblastoid cells (Namalva and BALL-1 cells) which have the characteristics of B lymphocytes are known to be good producers of human lymphoblastoid interferon (Hu IFN-alpha (Ly) ), there is only one report on IFN production by human T lymphoblastoid cells. The present study showed that Sendai virus could induce human T lymphoblastoid cells (RPMI 8402 cells) to produce IFN. On incubation with virus, IFN was detected after 9 h, and increased until 24 h. The IFN yield depended on the dose of Sendai virus. No superinduction was observed on treatment with BUdR or antimetabolites or on irradiation with ultraviolet light. The T cell line produced IFN even in serum-free medium. The IFN produced by RPMI 8402 cells (8402-IFN) was as stable at pH 2 and on heating at 60 C for 30 min as Hu IFN-alpha and Hu IFN-beta. 8402-IFN, Hu IFN-alpha and Hu IFN-beta showed the same kinetics of induction of the antiviral state of FL cells. The 8402-IFN was neutralized by anti-Hu IFN-beta serum, but not anti-Hu IFN-alpha serum, indicating that it was antigenically identical with Hu IFN-beta. MDBK cells were very sensitive to Hu IFN-alpha, but not to this IFN or Hu IFN-beta, but L929 and RK-13 cells were equally sensitive to 8402-IFN, Hu IFN-alpha and Hu IFN-beta. The molecular weight of this IFN was estimated as 21,000 and 16,500 daltons by SDS-PAGE and Sephadex G-100 gel chromatography, respectively.

Cell Line↗
PubMed1982

Antibody lysis of human hematopoietic cells in the absence of complement and effector cells.

The incubation of K-562 leukemia cells with specific goat immunoglobulin resulted in gradual cytolysis in the absence of complement and effector cells. Optimal lysis (100%), reached in 3-4 days, occurred when the concentration of the antibody in the culture medium was about 20 micrograms/ml containing an initial inoculum of 15,000 cells. A lower concentration (10 micrograms/15,000 cells/ml) led to partial cytolysis; the surviving cells, cultured in fresh medium, were still vulnerable to antibody lysis (30 micrograms/ml), thus indicating the lack of an apparent resistant cell population. The amount of free antibody in the culture medium diminished rapidly and very little or none was detectable after 2-3 hours of incubation with K-562 cells. The immune gamma-globulin also inhibited colony formation of K-562 cells in soft agar. The immune gamma-globulin, absorbed extensively with normal blood cells, to pluripotent K-562 cells, cross-reacted with several human malignant hematopoietic cell lines tested. These cells included T-lymphoblasts (JM and MOLT-4), promyelocytes (HL-60), Burkitt's lymphoma cells (RAJI), myeloblasts (KG-1 and KG-1a), and multiple myeloma cells (K-737). The absorption of the immune gamma-globulin with an equal number of cells from each of the cell lines assayed diminished but did not completely remove the antibody responsible for cytolysis of K-562 target cells. Thus, the K-562 leukemia blasts appear to possess common and specific antigens that may be only partially expressed on other committed leukemia cell lines studied. For this reason, the cytolytic activity of the immune gamma-globulin could only be removed by absorption with K-562 cells possessing a complete array of antigens solely present on pluripotential hematopoietic cells.

Antibodies↗
PubMed1982

[Antigenic types of interferons produced by several human lymphoblastoid cells].

There are two antigenic types of human virus-induced interferons (IFNs): one is a human leukocyte IFN (Hu IFN-alpha and the other is a human fibroblast IFN (Hu IFN-beta). We examined the antigenic types of IFNs produced in several human T and B lymphoblastoid cell lines through the induction by Sendai virus. We could produce IFNs in the several human T lymphoblastoid cells (RPMI 8402, Peer, JM and CCRF-CEM) and in several human B lymphoblastoid cells (BALL-1, B35M, DG-75 and ARH-77), and examined antigenic types of the IFNs produced. The results showed that all T lymphoblastoid cells produced Hu IFN-beta. On the other hand, the antigenic types of IFN were dependent on each cell line in human B lymphoblastoid cell lines. The IFN produced in BALL-1 cell was only one type of Hu IFN-alpha. The IFNs produced in ARH-77 and DG-75 cells contained two types of the Hu IFN-alpha and Hu IFN-beta. The IFN produced in B35M cell was only one type of Hu IFN-beta. It was concluded that human T lymphoblastoid cell produced Hu IFN-beta, and that the first type of human B lymphoblastoid cell produced only Hu IFN-alpha, the second type produced Hu IFN-alpha and Hu IFN-beta and the third type produced only Hu IFN-beta.

B-Lymphocytes↗
PubMed1982

Lack of autologous mixed lymphocyte reaction in patients with chronic lymphocytic leukemia: evidence for autoreactive T-cell dysfunction not correlated with phenotype, karyotype, or clinical status.

In the present study, there was a complete lack of autologous MLR between responding T cells or T subsets and unirradiated or irradiated leukemic B cells or monocytes in all 20 patients with CLL, regardless of disease status, stage, phenotype, or karyotype of the disease. The stimulating capacity of unirradiated CLL B cells and CLL monocytes or irradiated CLL B cells was significantly depressed as compared to that of respective normal B cells and monocytes in allogeneic MLR. The responding capacity of CLL T cells was also variably lower than that of normal T cells against unirradiated or irradiated normal allogeneic B cells and monocytes. The depressed allogeneic MLR between CLL B cells or CLL monocytes and normal T cells described in the present study could be explained on the basis of a defect in the stimulating antigens of leukemic B cells or monocytes. The decreased allogeneic MLR of CLL T cells might simply be explained by a defect in the responsiveness of T lymphocytes from patients with CLL. However, these speculations do not adequately explain the complete lack of autologous MLR in these patients. When irradiated CLL B cells or irradiated CLL T cells were cocultured with normal T cells and irradiated normal B cells, it was found that there was no suppressor cell activity of CLL B cells or CLL T cells on normal autologous MLR. Our data suggest that the absence or dysfunction of autoreactive T cells within the Tnon-gamma subset account for the lack of autologous MLR in patients with CLL. The possible significance of the autologous MLR, its relationship to in vivo immunoregulatory mechanisms, and the possible role of breakdown of autoimmunoregulation in the oncogenic process of certain lymphoproliferative and autoimmune diseases in man are discussed.

Adult↗
PubMed1982

Macrophage differentiation of human precursor B-cell line by phorbol ester and colony-stimulating factor.

A human round cell line, KLM-2, is considered to be precursor B-cell by immunologic surface marker analysis and histochemical studies. The effect of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and/or colony-stimulating factor (CSF) on KLM-2 cells was investigated. KLM-2 cells became adherent to the bottom of the flask on day 2 after liquid culture with TPA, and the number of macrophage-like adherent cells (mAC) with pseudopodia showed a TPA dose-dependent increase to the peak level on day 3 or 4. The mAC had a phagocytic activity. The suspending cells were positive for alpha-naphthyl acetate esterase (NAE) up to about 50% in culture with TPA. A TPA concentration of 100 ng/ml was optimal to provide a maximum number of mAC and NAE-positive suspending cells. Particularly, such effects of TPA on KLM-2 cells were markedly accelerated in the presence of CSF. These findings indicate macrophage differentiation of KLM-2 cells in the presence of TPA and CSF. KLM-2 cells may be a bipotential stem cell line differentiating into B-cell and monocyte-macrophage lineages.

B-Lymphocytes↗
PubMed1982

Antigenic types of various human lymphoblastoid cell interferons.

There are two antigenic types of human virus-induced interferon (IFN): human leukocyte IFN (Hu IFN-alpha) and human fibroblast IFN (Hu IFN-beta). Production of IFNs in various human T, B and non-T non-B lymphoblastoid cell lines were induced with Sendai virus and the antigenic types of these IFNs were determined. All the IFNs produced in human T lymphoblastoid cell lines (RPMI 8402, Peer, JM and CCRF-CEM cells) were antigenically identical to Hu IFN-beta. On the other hand, the antigenic type of IFN produced in human B lymphoblastoid cell lines was different in each cell line. IFNs produced in BALL-1 and RPMI 6410 cells were Hu IFN-alpha, but IFN produced in B35M cell was Hu IFN-beta. IFNs produced in DG-75 and ARH-77 cells consisted of both Hu IFN-alpha and Hu IFN-beta. IFNs produced in non-T non-B lymphoblastoid cell lines (NALM-16 and U-937 cells) also consisted of Hu IFN-alpha and Hu IFN-beta. Thus the antigenic types of IFNs produced in human lymphoblastoid cells reflect certain characteristics of the producer cells. The factors that determine which antigenic type of IFN is produced have not yet been clarified.

B-Lymphocytes↗
PubMed1982

The presence of monoclonal cytoplasmic immunoglobulins in leukemic B cells from patients with chronic lymphocytic leukemia.

It has generally been assumed that CLL B cells do not contain cytoplasmic immunoglobulin (Cylg), although these cells express surface membrane immunoglobulin (Smlg). The present study, in which Smlg and Cylg determinations were performed simultaneously using leukemia B cells from 20 patients with CLL, demonstrates that Cylg staining was detectable in each CLL cell population examined and that the intensity of cytoplasmic immunofluorescent staining in each instance was moderate to strong. Both Smlg and Cylg light chain determinations indicated monoclonality in all 20 cases. The heavy chain class of Smlg and Cylg in each CLL cell population was not uniformly comparable, however. The majority (15 cases) of CLL B cells contained mu heavy chain Cylg and mu and delta heavy chain Cylg was demonstrated in the remaining 6 cases. In contrast, the Smlg phenotypes appeared heterogeneous with both gamma and alpha chain determinants found associated with mu or mu and delta chains on the same leukemia cell populations. This apparent polyclonal Smlg staining pattern was most likely due to nonspecific adsorption of the patient's own serum Ig by Fc receptors on CLL B cells. It is concluded that the great majority of CLL B cells contain detectable Cylg and that Cylg determination is superior to Smlg phenotyping in documenting the monoclonality of CLL.

B-Lymphocytes↗
PubMed1982

Changes in clonal expression during the course of acute leukemia: possible subsets in childhood leukemia.

We studied cell surface markers and chromosomes in the leukemia cells of a boy with the initial diagnosis of acute lymphocytic leukemia during 18 months from diagnosis to demise. During this time he received induction therapy, underwent bone marrow transplantation, and relapsed. The leukemia cells expressed three membrane phenotypes during different stages of disease: T-cell at diagnosis; T-cell, B-cell, and monocyte during the induction period; T-cell in the first relapse after bone marrow transplantation; and T-cell and B-cell during the terminal stage. Some cells expressed markers of two cell types, indicating a common origin of these cells. Cytogenetic studies during post-transplantation relapse showed abnormal marker chromosomes that indicated two major sublines. However, there was enough sharing of other aberrant chromosomes to suggest that these two populations presented sublines within the same neoplastic clone. We suggest that these leukemia cells were derived from a pluripotential cell prior to differentiation into cells of the lymphoid and monocytic series. This particular case may represent a subset of acute leukemia and may account for the resistance to conventional therapy.

B-Lymphocytes↗
PubMed1981

Marker profiles of human leukemia and lymphoma cell lines.

By means of the multiple marker analysis, a total of 55 human leukemia-lymphoma cell lines which included 15 T-cell, 30 B-cell, four myelomonocytic-cell, and six non-T, non-B cell lines was characterized for their marker profiles. The multiple markers used included a number of cell surface markers as detected by either rosette or immunofluorescence tests, enzyme assays, cytogenetic analysis, and certain functional assay. Based on the criteria previously defined it was found that all the cell lines were proved to represent original leukemia-lymphoma of ALL, AML, CLL, CML in blastic phase or variety of lymphomas. The monoclonality, a "frozen" state at a specific state of differentiation-maturation, and cytogenetic marker in each leukemia-lymphoma cell line were remarkable common properties and were stable for years of cultivation. Similar, if not identical, general characteristics were observed in the study on 344 cases of uncultured fresh leukemia-lymphomas by the multiple marker analysis. While no single marker specific to any type of tumor was found, the study offers not only a basis for better understanding of the biology of leukemia-lymphoma but also an insight into normal hematopoietic cell differentiation in man.

Antigens, Neoplasm↗
PubMed1981

Chromosomes and causation of human cancer and leukemia. XLV. Chromosome patterns in stimulated lymphocytes of chronic lymphocytic leukemia.

In the present study the B-cell mitogens from Epstein-Barr virus (EBV) and lipopolysaccharide-B from E. coli (LPS) were used to stimulate the blood cells of 27 patients with chronic lymphocytic leukemia (CLL) to undergo mitosis. In 14 CLL cases EBV was used as the only mitotic stimulant and in 5 cases both EBV and LPS. In 8 cases neither EBV nor LPS caused mitotic cells to appear. In 9 cases the cells were characterized by abnormal clones, including 5 cases with +12 (including 1 case with a possible Philadelphia chromosome), 2 cases with 14q+ and 1 case with an i(17q). No correlation was found between the types of surface membrane immunoglobulins and the chromosome abnormalities in the leukemic lymphocytes. The effects of EBV on normal lymphocytes were also investigated and found to be nonspecific (e.g., tetraploidy), although in every case EBV caused a definite increase in the mitotic index. The results indicate that 1) EBV and LPS stimulate leukemic cells of CLL to undergo mitosis, 2) about 50% of the patients have clonal abnormalities in the leukemic cells, and 3) CLL is associated with karyotypic changes seen in lymphoma (14q+), although the most common anomaly in our material was a +12.

Aged↗
PubMed1981

Membrane differentiation in human myeloid cells: expression of unique profiles of cell surface glycoproteins in myeloid leukemic cell lines blocked at different stages of differentiation and maturation.

Cell surface glycoprotein structures of human leukemic cell lines blocked at various stages of myeloid (granulocyte--monocyte) differentiation and maturation were characterized. Cell lines representing early stages of differentiation and maturation such as KG1a, KG1 and those representing later stages such as ML-1-3 and HL-60 were found to express discretely different sets of cell surface glycoproteins. The proteins (such as Gp130) present on mature granulocytes appear only in cell lines representing later stages of maturation. The quantity and structure of the carbohydrate chains susceptible to endo-beta-galactosidase ("lactosaminoglycan") are also significantly different among cell lines. A small quantity of essentially unbranched lactosaminoglycan is present on KG1a and KG1 cells, whereas a significant quantity of branched lactosaminoglycan is present on the more mature ML-1-3 and HL-60 cells. The process of myeloid maturation from promyeloblast to granulocyte is accompanied by the disappearance of the 105- and 95-kilodalton glycoproteins, the appearance of Gp130, and a great increase in the quantity and branching structure of lactosaminoglycan. These results are discussed together with our previous findings on erythroid differentiation and maturation.

Cell Differentiation↗
PubMed1981

Comparison between agar and methylcellulose cultures of human leukemic cells.

A comparison was made between the agar and methylcellulose culture systems with respect to their ability to support the clonal growth of leukemic cells obtained from patients with acute myeloblastic leukemia, acute lymphoblastic leukemia, and chronic myelogenous leukemia in blastic crisis. The number of clusters and/or colonies formed and the morphology of the cells within them varied from patient to patient. Nevertheless, no significant difference between the two culture systems within given leukemic specimens was found. No significant differences were noted among three different conditioned media used as sources of colony-stimulating activity. Most of the cells within clusters and colonies were identified to be immature members of granulocyte-macrophage series or to be indistinguishable from the preculture leukemic blast cells by morphological and cell surface marker studies. Cells from myeloid crisis in chronic myelogenous leukemia grew well in the cultures, but cells from lymphoid crisis did not proliferate.

Adult↗