Proceedings: Bacteriophage ms2 rna: nucleotide sequence determination of the A protein gene.
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Base Sequence↗
Biomedical subjects
J Merregaert
Publications and source records attributed to J Merregaert.
Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron.
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Base Sequence↗
Recent progress in the sequence determination of bacteriophage MS2 RNA.
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Amino Acid Sequence↗
The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA.
RNA fragments of different chain length, each containing the 5'-terminal guanosine tetraphosphate (pppGp) of bacteriophage-MS2 RNA, have been isolated from partial ribonuclease digests of the viral RNA. The longest fragment overlaps with the ribosomalbinding site of the A-protein cistron. The base sequence has been established for the major part. The results directly confirm that the A-protein cistron is closest to the 5'-terminus. Its initiating (AUG) codon starts at position 130, being preceded by an untranslated sequence of 129 nucleotides.
Autoradiography↗
Position of the first initiation codon in the RNA of bacteriophages MS2 and R17.
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Base Sequence↗