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J Meek

Publications and source records attributed to J Meek.

At least 55 records · Page 3Linked to original sources
PubMed1987

Isolation, identification, and synthesis of 2-carboxyarabinitol 1-phosphate, a diurnal regulator of ribulose-bisphosphate carboxylase activity.

The diurnal change in activity of ribulose 1,5-bisphosphate (Rbu-1,5-P(2)) carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing); EC 4.1.1.39] of leaves of Phaseolus vulgaris is regulated (in part) by mechanisms that control the level of an endogenous inhibitor that binds tightly to the activated (carbamoylated) form of Rbu-1,5-P(2) carboxylase. This inhibitor was extracted from leaves and copurified with the Rbu-1,5-P(2) carboxylase of the leaves. Further purification by ion-exchange chromatography, adsorption to purified Rbu-1,5-P(2) carboxylase, barium precipitation, and HPLC separation yielded a phosphorylated compound that was a strong inhibitor of Rbu-1,5-P(2) carboxylase. The compound was analyzed by GC/MS, (13)C NMR, and (1)H NMR and shown to be 2-carboxyarabinitol 1-phosphate [(2-C-phosphohydroxymethyl)-D-ribonic acid]. Verification of structure was obtained by comparison of the inhibitory activity of the isolated compound with that of 2-carboxy-D-arabinitol 1-phosphate synthesized in vitro. This compound (but not 2-carboxy-D-arabinitol 5-phosphate) inhibited Rbu-1,5-P(2) carboxylase in a way that was kinetically identical to that of the isolated, naturally occurring compound. The structure of the isolated compound differs from the Rbu-1,5-P(2) carboxylase transition-state analogue 2-carboxyarabinitol 1,5-bisphosphate only by the lack of the C-5 phosphate group. This difference results in a higher binding constant for the monophosphate (K(d) = 32 nM) compared with the bisphosphate (K(d) < 10 pM). The less tightly bound compound acts in a light-dependent, reversible regulation of Rbu-1,5-P(2) carboxylase activity in vivo.

Journal Article↗
PubMed1987

Preparation and properties of monoclonal and polyclonal antibodies to mouse epidermal growth factor (EGF) receptors: evidence for cryptic EGF receptors in embryonal carcinoma cells.

Monoclonal antibodies to mouse epidermal growth factor (EGF) receptor were prepared by the immunization of rats with receptor glycoprotein purified from mouse liver by affinity chromatography on immobilized EGF. Purified mouse EGF receptor retained EGF-inducible autophosphorylating activity and was antigenic in rats and rabbits. The monoclonal antibodies cross react very poorly with human EGF receptor, while polyclonal rabbit antibodies immunoprecipitate human, rat and mouse EGF receptor equally well. The rabbit antibody blocks EGF binding to mouse fibroblast cells and, at 20-fold higher concentrations, stimulates uptake of tritiated thymidine into DNA. This indicates that antibodies bind at or close to the EGF-binding site and can mimic the effects of the growth factor. None of the monoclonals bind at the EGF site of the receptor. Immunoprecipitation, immunoblotting, 125I-EGF cross linking, 125I-surface labelling, immunohistochemistry and autophosphorylation techniques were used to delineate the basis for the induction of EGF receptors when OC15 embryonal carcinoma (EC) cells differentiate into endodermal derivatives (END). EGF-stimulated autophosphorylation of a 170 X 10(3) Mr protein in solubilized OC15 EC cells is readily detectable, although intact EC cells do not bind or respond to EGF by all other tests. The results suggest that cryptic EGF receptors are present in EC stem cells, a finding with implications in development.

Animals↗
PubMed1986

Afferent and efferent connections of cerebellar lobe C1 of the mormyrid fish Gnathonemus petersi: an HRP study.

The corpus of the highly developed cerebellum of the weakly electric fish Gnathonemus petersi is differentiated into four lobes, numbered C1 to C4. The present paper deals with the extrinsic connections of the rostralmost lobe C1. Relevant nuclei were studied in normal histological material and HRP injections were placed in lobe C1, the neighbouring pedunculus valvulae, and the brainstem. The largest number of afferents to lobe C1 originates from the nucleus lateralis valvulae, a large nucleus of tightly packed small cells in the dorsal midbrain tegmentum. In Gnathonemus this nucleus encompasses nine subdivisions, of which the rostral, caudal, and exterolateral parts project in particular to lobe C1. Larger neurons in the dorsal midbrain tegmentum and presumed mesencephalic trigeminal neurons project to C1 as well. In the rhombencephalon, afferents to lobe C1 arise from the first funicular nucleus, the lateral reticular nucleus, and the inferior olive. Efferents of lobe C1 have been found to arise from a peculiar cell type in the Purkinje cell layer (so-called eurydendroid neurons) and to project predominantly to the nucleus of the fasciculus longitudinalis medialis, the nucleus reticularis superius and medius, and the trigeminal motor nucleus. Additional small projections terminate in the tectum mesencephali and in the nucleus reticularis inferior. Compared with other parts of the mormyrid cerebellum as well as with the cerebellum of other teleosts, the connections of lobe C1 appear to be quite restricted and specialized. In this respect the connections with the trigeminal nerve via the first funicular nucleus, the mesencephalic trigeminal nucleus, and the trigeminal motor nucleus are of particular interest. The absence of central cerebellar nuclei intercalated in the efferent cerebellar connections, in combination with the presence of a precerebellar nucleus (lateralis valvulae) involved in the afferent cerebellar connections, represents a remarkable difference between teleosts and other vertebrate classes.

Afferent Pathways↗
PubMed1986

Afferent and efferent connections of cerebellar lobe C3 of the mormyrid fish Gnathonemus petersi: an HRP study.

The present paper is devoted to the extrinsic connections of lobe C3 of the highly differentiated corpus cerebelli of the electric fish Gnathonemus petersi. For this purpose, HRP injections or gels were placed in distinct parts of lobe C3 or its peduncle, in the pretectal region, and in the eye. Moreover, the presence of serotonin and tyrosine-hydroxylase was studied with immunohistochemical methods. The afferent connections of the rostral and caudal part of lobe C3 appear to differ considerably. Although both parts receive comparable projections from two pretectal nuclei (termed nucleus geniculatus and dorsal anterior pretectal nucleus) and the inferior olive, they receive projections from different parts of the nucleus lateralis valvulae, a large cell mass in the midbrain tegmentum, composed of small, tightly packed neurons. The caudal part of lobe C3 receives a projection from the most rostromedial cap of cells of this nucleus, whereas the rostral cap of lobe C3 receives efferents from the neighboring, more caudolateral, zone of cells of the nucleus lateralis valvulae. The caudal part of lobe C3, but not its rostral part, receives an additional projection from a nucleus in the isthmus region, termed nucleus Q. This nucleus sends a collateral projection to the torus longitudinalis. The efferents of both parts of lobe C3 project to slightly different parts of the midbrain tegmentum and the nucleus reticularis superior, and originate at least partly from eurydendroid cells. None of the nuclei and fiber tracts labeled could be shown to contain serotonin or catecholamines. The connections of lobe C3, as revealed by the present study, are compared with those of other parts of the mormyrid cerebellum and with those of the corpus cerebelli of other teleosts, with emphasis on the homology and functional significance of pretectocerebellar connections, the topical order in the cerebellar projections of the nucleus lateralis valvulae, and the relations between the cerebellum and torus longitudinalis. Comparison of the cerebellar connections in different teleostean species suggests that the strong development and the considerable differentiation of the cerebellum of mormyrids are related to at least two types of changes in the extrinsic connections, i.e.: a redistribution or parcelling of connections and the development of connections specific for mormyrids.

Afferent Pathways↗
PubMed1985

A detailed morphometrical analysis of the pyramidal tract of the rat.

The present study is aimed at a quantitative analysis and comparison of the fibers of the pyramidal tract of the rat at two levels: the pyramis medullae and the second cervical segment. For this purpose both levels of the pyramidal tracts of 5 rats have been studied morphometrically at the ultrastructural level. Fiber numbers as well as fiber diameter distributions are presented for both myelinated and unmyelinated axons. At the level of the pyramis medullae the number of unmyelinated fibers (140,000 +/- 7000) exceeds the number of myelinated axons (103,000 +/- 6000). In contrast at the level of the second cervical segment the numbers of fibers of the axon populations studied are not significantly different, viz. 43,000 +/- 2000 myelinated and 35,000 +/- 8000 unmyelinated fibers. However, these numbers mean a significant decrease of myelinated axons (48%) compared with the pyramis medullae level and an even much larger decrease (75%) in the numbers of unmyelinated fibers. Diameter distributions, obtained from the minimal fiber diameter, show a similar, monomodal shape for all axon and myelin profiles. For unmyelinated axons the minimal diameter ranges from 0.05 to 1.21 micron with a mean of 0.18 +/- 0.03 micron. For myelinated axons a mean minimal diameter of 0.72 +/- 0.12 micron has been found (range 0.13-4.92 micron), whereas their diameter measured with myelin sheath measure 1.08 +/- 0.13 micron (range 0.25-6.03 micron). The average thickness of the myelin sheath is 0.2 micron and is strongly correlated to axon diameter. Furthermore, g-ratio has been computed at a mean of 0.65 and is fairly constant throughout the diameter spectrum. Several possibilities concerning the functional and anatomical qualities of the numerically important unmyelinated axon population in the pyramidal tract of the rat are discussed.

Animals↗
PubMed1985

Product of the cellular oncogene, c-fos, observed in mouse and human tissues using an antibody to a synthetic peptide.

The transforming gene of the osteosarcoma-producing FBJ murine sarcoma virus, v-fos, is homologous to a normal cellular gene, c-fos, in vertebrate species. Transcripts from the c-fos proto-oncogene accumulate to very high levels in late gestational mouse and human extra-embryonic tissues. We now report that these RNA transcripts are translated in these tissues. Rabbits were immunized with a synthetic peptide whose sequence is common to both c-fos and v-fos. After affinity purification on an immunosorbent containing the fos peptide (a nonapeptide), the antibody reacted with a component(s) in nuclei in sections of human and murine tissues and immunoprecipitated the v-fos gene product (p55) and a cellular protein of 39 kd (p39, complexed with fos) from lysates of metabolically-labelled virally transformed cells. Crude extracts of normal tissues contained major anti-fos-reactive proteins in the range of 55-60 kd as shown by protein blot analysis. Indirect immunofluorescence and immunoperoxidase staining showed that in addition to strong immunoreactive component(s) in the nuclei of extra-embryonic tissues of human and mouse, weaker reactions are detectable in all normal fetal and adult tissues tested. This demonstrates that fos-reactive protein is expressed in a wide variety of cells and tissues.

Amino Acid Sequence↗
PubMed1985

Transferrin in foetal and adult mouse tissues: synthesis, storage and secretion.

Transferrin is an important growth-promoting serum glycoprotein synthesized chiefly in the liver in adults. The transferrin found in the mouse foetus is thought to be wholly a product of the foetus itself and its synthesis starts at lest as early as the 7th day of gestation. The major sites of synthesis in mouse foetuses are the visceral yolk sac (VYS) and liver (Adamson, 1982). We now report that other murine foetal tissues synthesize readily detectable amounts, namely lung, spleen, spinal cord and rib cage. Very low levels are also synthesized by the brain, muscle and pancreas. We can detect no synthesis of transferrin in late foetal thymus, heart or skin although mid-gestation foetal skin may make a very small amount. No synthesis of transferrin can be detected in adult brain, lung and spleen, but approximately equal rates of synthesis are detected in adult liver and adult ear pinna. Transferrin is accumulated by foetal and adult tissues in widely varying amounts and these have been measured by enzyme-linked immunosorbent assays of extracts. In addition to VYS and liver, high levels of transferrin are found in foetal skin, lung and rib cage with lower amounts in spinal cord, spleen and muscle tissues. Tissues of the 15th day foetus accumulate the highest concentrations of transferrin. A role for the mediation of transferrin in the stimulation of growth and differentiation by interaction tissues is discussed.

Animals↗
PubMed1984

The ontogeny of epidermal growth factor receptors during mouse development.

In an attempt to understand the role(s) of epidermal growth factor (EGF) in vivo during murine development, we have examined the 125I-EGF binding characteristics of EGF-receptors in membrane preparations of tissues from the 12th day of gestation to parturition. Using autoradiography, the earliest time that we could detect EGF-receptors was on trophoblast cells cultured for 3 days as blastocyst outgrowths. Trophoblast eventually forms a large portion of the placenta, where EGF-receptors have long been recognized. We measured the number and affinity of EGF-receptors on tissues dissected from conceptuses from the 12th day of gestation in order to identify a stage when tissues may be most sensitive to EGF. Whereas the number of EGF receptors increases during gestation for all tissues examined, the affinity of the receptors declines for carcass and placenta and remains relatively unchanged for brain and liver. This suggests that EGF may function differently throughout development. Our hypothesis is that EGF (or its embryonic equivalent) initially stimulates proliferation in embryonic cells and then stimulates differentiation as the tissues mature. In the adult, its main role could be to stimulate tissue repair after damage.

Animals↗
PubMed1984

Dietary protein supplementation from vegetable sources in the management of chronic portal systemic encephalopathy.

In a controlled cross-over trial, we have compared a conventional 40-g protein diet (30 g animal and 10 g vegetable, diet A) with an 80-g vegetable-protein-supplemented diet (30 g animal and 50 g vegetable, diet B) in the treatment of six patients with chronic stable portal systemic encephalopathy, requiring dietary and lactulose therapy. Each diet was given, in random order, for 5 days in hospital. EEG, clinical indices of encephalopathy, and the plasma amino acid profile were assessed at the end of each treatment period. The increase in vegetable protein intake was associated with minor improvement in EEG and clinical performance in two patients, and no change in the others. Fasting plasma phenylalanine and tyrosine were higher on diet B [phenylalanine 108.6 +/- 9.3 (SEM) mumol/L versus 99.6 +/- 8.37, p less than 0.05 (paired t test); tyrosine 153 +/- 15.2 mumol/L versus 140 +/- 14, p less than 0.05). The plasma branched-chain amino acid levels did not change, and the branched chain/aromatic amino acid ratio (BCAA/AAA) was lower on diet B (p less than 0.02). Fecal weights were not significantly altered. These results indicate that patients with chronic portal systemic encephalopathy are tolerant of protein supplementation from vegetable sources. A minor improvement in parameters of encephalopathy was seen in some individuals, despite a lowering of BCAA/AAA which some investigators have thought important in the pathogenesis of encephalopathy.

Adult↗
PubMed1983

Functional anatomy of the tectum mesencephali of the goldfish. An explorative analysis of the functional implications of the laminar structural organization of the tectum.

The present paper is aimed at an exploration of the possible functional significance of the laminar organization of the goldfish tectum at both the cellular and the synaptic level. For this purpose (1) the data concerning the structure of the teleostean tectum are surveyed, (2) a conceptual framework of the intratectal connectivity in the goldfish is proposed, (3) the electrophysiological data concerning the teleosteam tectum are surveyed and (4) the degree of correlation between the structural and physiological data available is discussed. Apart from the retina, tectal afferents originate from at least 10 other brain centers. At least 5 of these projections appear to be topographically organized. Tectal afferents, neurons as well as synapses reveal a characteristic intratectal lamination pattern. Tectal efferents project to at least 10 brain centers, and have until now been shown to arise from 6 cell types. The structural data surveyed allow the construction of a conceptual framework of tectal circuitry on the basis of 3 starting points. (1) The existence of at least 8 presynaptic zones or laminae, each containing a characteristic set of presynaptic structures (afferents and axons of interneurons). (2) The fact that the tectal postsynaptic structures (somata and dendrites of tectal neurons) each have a characteristic location, extension and synaptic density, which determines the relative importance of the different presynaptic zones for each cell type. (3) The laminar specificity hypothesis, which implies that presynaptic structures that coexist in a particular presynaptic zone terminate without preference on all types of postsynaptic structures within that zone. The conceptual framework of tectal circuitry is quantified in terms of connectivity index and connective importance. Analysis of the framework constructed leads to a detailed description of the intratectal pathways involved in the processing of the 4 main streams of tectal input (i.e. visual, toral, telencephalic and 'deep' input). It was concluded that the laminar organization of the tectum is primarily relevant for multimodal integration and that the tectal cell types each receive a characteristic sample out of the multimodal information available in the different tectal layers.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1981

A Golgi-electron microscopic study of goldfish optic tectum. I. Description of afferents, cell types, and synapses.

A study of goldfish optic tectum was performed with conventional electron microscopy and with the Golgi-EM technique described by Fairén et al. ('77). Five types of tectal afferents, three types of interneurons and three types of efferent neurons were investigated. Afferents from the torus longitudinalis, which terminate in the marginal layer, contain round synaptic vesicles with a mean diameter of 43 nm. Optic afferents, which terminate in the superficial gray and plexiform layer, are characterized by pale mitochondria with dilated cristae and round vesicles with a mean diameter of 49 nm. Afferents of unknown origin, terminating in several tectal layers, can be subdivided in three types; one containing round vesicles and two containing pleomorphic vesicles with different degrees of ellipticity. The three types of interneurons studied (type I, III and XIV, of Meek and Schellart, '78) were selected on basis of their high frequency of occurrence. The apical dendrites of type I neurons make many synaptic contacts with the marginal axons. All three types have dendrites in the superficial gray and plexiform layer making contacts with optic nerve terminals. In addition, their dendrites and cell bodies make synaptic contacts with several types of unidentified presynaptic elements. The axon terminals of type I and of type XIV contain round vesicles with a mean diameter of 45 and 46 nm respectively. Three of the four types of efferent neurons present in the goldfish tectum were studied (type VI, XII and XIII). Two of them make contact with optic terminals (type VI and XII) and two make contact with tectal afferents of unknown origin in the central white layer or in the lower part of the central gray layer (type XII and XIII). The axons of all three types become myelinated at some distance from their origin. Their initial unmyelinated parts are covered with a so-called "outer surface coating", have no collaterals and are occasionally (type VI and XII) or frequently (type XIII) postsynaptic to other elements. The archiform axons of type XIII and to a lesser extent also the sherpherds-crook shaped axons of type XII, have a close apposition to looping and narrowing dendrites in the inner plexiform layer. The present results concerning neuronal circuitry of the goldfish optic tectum are summarized in a tentative scheme.

Animals↗
PubMed1981

A Golgi-electron microscopic study of goldfish optic tectum. II. Quantitative aspects of synaptic organization.

The size, density, and number of the synaptic contacts of three types of interneurons (types I, III, and XIV of Meek and Schellart, '78) and three types of efferent neurons (types VI, XII, and XIII) of the goldfish optic tectum were quantified by means of a quantitative stereological study of Golgi-EM serial sections. Furthermore, an estimation was made of the percentage of optic terminals on these six cell types and of the ratio between terminals with pleomorphic and terminals with round vesicles. The mean density of contacts per receptive component (i.e. the cell body and the different parts of the dendritic tree) varies from 0 to 100 per 100/micrometer2 surface, corresponding to 0-8% receptive surface. Each cell type has a characteristic average density as well as a characteristic density distribution along the distinct components. This suggests that the receptive components of the tectal cell types investigated have a predetermined density and that a morphological classification of tectal cells has functional relevance. The mean length of the contact zones in the ultrathin sections varies from 213 to 332 nm for identified postsynaptic elements and from 188 to 293 nm for identified presynaptic elements. The size of the contacts on the distinct receptive components appears to be primarily related to the tectal lamination pattern. Distinct types of axons, however, have characteristic mean sizes of contacts. This might suggest that the size of the contacts, contrary to their density, is primarily determined by the presynaptic elements. The mean number of synaptic contacts calculated per cell type is as follows: type XIV, 200; type III, 450; type VI, 1,400; type I, 2,100; type XII, 4,200; and type XIII, 5,400. Multiplication of these numbers with the number of cells per tectal half shows that the population of type XIV cells has by far the most synaptic contacts, since their low number of synaptic contacts is clearly overruled by their high frequency of occurrence. Optic terminals, identified by their characteristic mitochondria and large round vesicles, appear to contribute to about 10-20% of the contacts on identified post-synaptic elements in layer 5. The ratio between presynaptic elements with pleomorphic vesicles and those with round vesicles shows a slight tendency to increase when the distance to the origin of the axon decreases. It is concluded that a combination of the Golgi-EM technique with quantitative stereological methods appears well suited to the study of the synaptic organization of brain centers, and that combination of quantitative Golgi-EM with neuronal tracing methods (degeneration, HRP, autoradiography) offers good prospects for detailed investigations of neuronal connectivity.

Animals↗
PubMed1980

The effect of kainic acid on the hippocampal content of putative transmitter amino acids.

The time courses of the changes in the contents of GABA (gamma-aminobutyric acid), glutamate, aspartate, taurine, glycine and alanine were measured in both hippocampi of rats which were rejected in one hippocampus with kainic acid (KA). The contents of these amino acids were measured with high performance liquid chromatography. The hippocampal contents of GABA, glutamate, aspartate and taurine were reduced homolaterally to the KA injection; in contrast, the contents of glycine and alanine failed to change. The extent of the reductions of GABA, glutamate, aspartate and taurine was dependent on the size of the lesion caused by KA. The greatest decrease occurred after two simultaneous injections of KA, in dorsal and ventral hippocampus. An analysis of the amino acids at different levels of the hippocampus after dorsal injections of KA showed that in the hippocampus the amino acidergic axons do not travel longitudinally. This study suggests that GABA, glutamate, aspartate and taurine are preferentially located in intrinsic hippocampal neurons with short axons.

Amino Acids↗
PubMed1978

A Golgi study of goldfish optic tectum.

A study of goldfish optic tectum was performed with rapid-Golgi, Golgi-Kopsch and a modified Golgi-Cox impregnation which proved quite suitable to impregnate cells in the middle tectal layers and to study more closely axonal properties. Fifteen cell types are distinguished, based upon the position of dendritic trees and axonal properties. Two cell types are found with dendrites in the marginal layer: type I with an axon terminating in the central gray layer and type II without an impregnated axon. Three cell types (III, IV and V) have dendrites in a single, specific tectal layer and an axon terminating within the tectum. Five cell types (VI-X) have dendrites in two horizontal planes. Two of them have myelinated axons leaving the tectum, whereas the axons of the remaining three types project to different tectal layers. While these first ten cell types have dendrites almost exclusively in the superficial half of the tectum, the remaining five types have dendrites in deeper layers too. This especially holds for the most conspicuous tectal cells (types XII and XIII), which have dendritic trees branching at three or more horizontal levels and a myelinated axon leaving the tectum, with sometimes a very peculiar course (XIII1). Also type XI has three or more dendritic trees, but its axon was not found. The numerous cells with cell bodies in the deepest tectal layer (type XIV) have dendrites and axonal terminations anywhere in the tectum, except in the most superficial and the deepest layer. However, most dendrites occur in the optic layers, whereas the axons, always originating from the dendritic shaft in the superficial tectal half, generally terminate in the middle tectal layers. Type XV cells have their soma in the deepest tectal layer as well, but their dendrites do not reach the optic layers. Per tectal lobe the following numbers are estimated: type I : 5,000-20,000 neurons; Type III : 2,500-10,000; types IV--XIII : each 500-2,000 and type XIV : 1,000,000-2,000,000. The total number of myelinated tectal efferents is estimated at 2,000-8,000. Comparison with other Golgi studies in teleosts leads to the conclusion that the tecta of these species of fish are basically similar.

Animals↗