A Simple Splint for Wrestler's Ear.
Explore the source record for details and available documents.
Journal Article↗
Biomedical subjects
J Mason
Publications and source records attributed to J Mason.
The effect of trithiomolybdate and some selenium compounds upon 109Cd labeled rat metallothionein in vitro: the possibilities for cadmium chelation therapy.
The main binding protein for 109Cd was metallothionein after in vitro incubation of various tissue cytosol preparations obtained from rats supplemented with zinc. The exception was heart cytosol where the label was associated with higher molecular weight proteins. The metallothionein-bound 109Cd was sensitive to trithiomolybdate and moved too higher molecular weight proteins, presumably because of the creation of new stronger ligands by the association of thiomolybdate with these proteins. The 109Cd binding was affected by selenate, selenite, and selenide while molybdate, sulphate, and thiosulphate were ineffective. It is proposed that thiomolybdates should be investigated for use in the therapy of in vivo cadmium toxicity because they can remove the accumulated metal from metallothionein.
Animals↗
Serum creatinine or glomerular filtration rate for monitoring cyclosporin therapy.
Explore the source record for details and available documents.
Creatinine↗
The invisible-obstacle race.
In some respects, things are getting worse, not better, for women in science. Positive measures need to be taken for progress towards genuine equality of opportunity.
Europe↗
A comparison of the effects of penicillamine, trientine, and trithiomolybdate on [35S]-labeled metallothionein in vitro; implications for Wilson's disease therapy.
The synthesis of radiolabeled metallothionein was induced in rats in vivo by the injection of CuSO4 and [35S]-cysteine. Treatment of "cold" rat liver cytosol "spiked" with purified [35S] metallothionein with Penicillamine and Trientine showed that even at relatively high concentrations (up to 50 mg/g liver, wet weight), these compounds had no effect on the copper peak or the position of the [35S] label in the cytosol eluate after Sephadex G-75 gel filtration. By contrast, incubation of the "spiked" liver cytosol with Trithiomolybdate, even at relatively low concentrations (0.5 mg/g liver, wet weight), resulted in a transfer of metallothionein copper to high molecular weight protein fractions; the position of the [35S] apoprotein was unaffected. This copper "stripping" effect on metallothionein supports clinical and other evidence that thiomolybdates have a genuine decoppering effect in vivo whereas Penicillamine and Trientine have another mode of action and indicates that thiomolybdates might provide a more rational alternate therapy for Wilson's disease patients.
Animals↗
Evolution of flight nursing and the National Flight Nurses Association.
Explore the source record for details and available documents.
Aircraft↗
Suppression of the abnormal phenotype of Salmonella typhimurium rfaH mutants by mutations in the gene for transcription termination factor Rho.
Mutations in the rfaH gene have previously been shown to cause premature termination of transcription of the traYZ operon of the F factor and also to prevent expression of the rfaGBIJ gene cluster of Salmonella typhimurium. In the present study, mutants were selected for their ability to restore the normal pattern of rfaGBIJ function. On the basis of this initial section, several classes of extragenic suppressor mutants were isolated that completely or partially corrected the Tra- and Rfa- phenotypes of the prototype rfaH mutant. The suppressor mutations included mutations in rho and mutations that mapped in or close to rpoBC. Other suppressor mutations were located elsewhere on the chromosome, presumably identifying other genes that play a role in the RfaH-mediated transcriptional regulation.
Gene Expression Regulation, Bacterial↗
HIV/AIDS education in a prenatal clinic: an assessment.
An HIV/AIDS education and counseling program was integrated into the routine medical care of women attending the prenatal clinic of a major urban, inner-city, teaching hospital that serves mostly indigent minority women in an area hard hit by the HIV/AIDS epidemic. Pre- and post-intervention questionnaires were administered to a consecutive historical Control Group (n = 98) who did not receive the HIV/AIDS information and an Intervention Group (n = 515) who received all information. The data support our hypothesis that an HIV/AIDS education program would increase the level of general knowledge, but fail to support our hypothesis of a positive effect on attitudes around HIV-antibody testing and an increase in desire for voluntary testing. Our hypothesis that women reporting more risk behaviors would be more likely to agree to HIV-antibody testing was only partially supported.
Acquired Immunodeficiency Syndrome↗
Incorporating HIV education and counseling into routine prenatal care: a program model.
This article reports how a prenatal clinic in a major urban teaching hospital has developed and integrated an HIV education and counseling program into routine prenatal care. The patient population served are predominantly minority women living in an inner-city community that has been disproportionately affected by the AIDS epidemic. Implementation of the patient program has required training and support for all professional staff. Staff training served as a foundation for this comprehensive patient program, which has reached all prenatal patients regardless of risk behavior. The program has succeeded in involving a large population of women in an educational program, has identified HIV-1 seropositive pregnant women through voluntary testing, and has provided them with the necessary medical and social work services. Principles of program development are identified for use in other settings.
Ambulatory Care↗
Cyclosporine and the renin-angiotensin system.
Much is known about the renin-angiotensin system during cyclosporine treatment: plasma renin or prorenin activity seems to be raised in man and animals; hyperplasia of the juxtaglomerular apparatus has been reported in man and animals; renin concentration is known to be increased in animal kidneys; renin-angiotensin-like alterations in vascular contractility have been identified in animal vessels. Since the major functional side effects of cyclosporine are systemic vasoconstriction and renal vasoconstriction, the renin-angiotensin system could be involved. Also, an infrequent complication in man, vasculopathy, develops only in the afferent arteriole, where renin production is most abundant. Hence, this information is now reviewed and incorporated into a single pathophysiological concept, with the implication that renin activation plays a central role in the pathogenesis of cyclosporine-induced functional and structural lesions, merits further attention.
Animals↗
Obligations of kinship in contemporary Britain: is there normative agreement?
This article explores the extent to which there is normative agreement in contemporary Britain about the 'proper thing to do' for relatives. Using quantitative survey data generated using the 'vignette technique', the authors assess how far certain policy and sociological assumptions about appropriate kinship obligations hold up to empirical scrutiny. They argue that there is not a straightforward consensus about a set of normative principles but that it is possible to identify patterns of normative agreement. These vary more in line with the circumstances specified in the vignettes than with the social characteristics of the respondents. There is more evidence of a consensus over procedures - that is what factors people should take into account in working out the proper thing to do for relatives - than over the substance of what should be done. The authors conclude that people do not carry around with them stable sets of values and meanings about obligations to kin, but construct them when they have to out of various materials available.
England↗
The dexamethasone suppression test and thyrotropin-releasing hormone stimulation test in posttraumatic stress disorder.
Male veterans with posttraumatic stress disorder (PTSD) (n = 11), including 6 with concurrent major depressive disorder (MDD), were compared to veterans with MDD alone (n = 18) and to 28 controls in their response to the dexamethasone suppression test (DST) and thyrotropin-releasing hormone (TRH) stimulation tests. We found higher levels of 4 PM serum cortisol and lower peak thyroid-stimulating hormone (TSH) response to TRH in the MDD patients than in either the PTSD patients or controls, in spite of equivalent levels of depression for MDD and PTSD. DST suppression (cortisol less than 5 mg/dl) occurred in 90% of control, 90% of PTSD, and 78% of MDD subjects, whereas TRH blunting (dTSHmax less than 7 microU/ml) occurred in 28% of control, 27% of PTSD, and 67% of MDD subjects. Rather than blunting, four PTSD patients (36%) and only 10% of the control and MDD subjects had high TSH responses (13-24 microU/ml), which may be linked to high noradrenergic activity, since subclinical hypothyroidism seemed unlikely.
Adult↗
p53 is associated with p34cdc2 in transformed cells.
The normal functioning of p53 is thought to involve p53 target proteins. We have previously identified a cellular 35 kd protein associated with p53 and now report evidence identifying this 35 kd protein as p34cdc2, product of the cell cycle control cdc2 gene. The association between p53 and p34cdc2 was detected in SV3T3 and T3T3 cell lines, both expressing the wild-type p53 phenotype, and in 3T3tx cells, expressing 'mutant' p53 phenotype. Binding of the mutant p53 phenotype with p34cdc2 was greatly reduced relative to wild-type. Complexes of p53-p34cdc2 may represent inactivation or activation of either component. The p34cdc2 kinase functions at cell cycle control points and is necessary for entry and passage through mitosis. It also operates in G1 and is involved in the commitment of cells into the proliferative cycle. Since we were unable to detect p53-p34cdc2 complexes in mitotic cells we propose that the interaction between p53 and p34cdc2 may be functional in cell growth control, possibly to promote or to suppress cell proliferation.
Animals↗
The pathophysiology of Sandimmune (cyclosporine) in man and animals.
Part II: The side-effects of Sandimmune that have also been observed clinically include hepatic dysfunction, glucose intolerance, thrombo-embolic complications and nervous system disorders. To determine the cause and significance of such effects, the actions of Sandimmune on the liver, the pancreas, on hematostasis and the nervous system were examined. Comparisons were made between animal and human data obtained in vivo and in vitro, and the clinical setting under which the side-effects occur was analyzed. The actions of Sandimmune on the liver seem to reflect mostly a cholestasis with a small depression in protein synthesis and a mild disturbance in lipid metabolism of uncertain origin. The action of Sandimmune on the pancreas suggests insulin resistance and possibly a secretory disturbance, with no evidence for depressed insulin synthesis, except in animals at high doses. Sandimmune does not seem to promote thromboembolism in man, although fibrinolysis may be depressed and platelet aggregation can be enhanced. The effects of Sandimmune on the nervous system are unclear, for tremor is common but of uncertain origin, whereas seizures and encephalopathy are rare and invariably associated with other risk factors.
Animals↗
The pathophysiology of Sandimmune (cyclosporine) in man and animals.
Part I: The side-effects of Sandimmune that have been of most significance clinically are renal dysfunction, renal vascular damage and arterial hypertension. To examine the nature and the origin of such effects, the actions of Sandimmune on the renal tubule, the renal vessels and systemic vessels have been analyzed. To evaluate whether common vasoconstrictory systems may be involved, changes in the renin-angiotensin-aldosterone system and prostaglandin-thromboxane system have been assessed. Comparison between animal and human data obtained in vivo and in vitro shows the actions of Sandimmune on the renal tubule to be modest and involve only a few specific effects. The major action of Sandimmune is on the vessels, vasoconstriction being the major cause of renal dysfunction and also the cause of arterial hypertension. Neither the circulating renin-angiotension-aldosterone system nor the prostaglandin-thromboxane system is clearly responsible for vasoconstriction. Although not itself a vasoconstrictor, Sandimmune seems to modulate the constrictory and dilatory response to other agents in several vascular beds.
Animals↗
Renal side-effects of cyclosporin A.
Cyclosporin A (CyA) has multiple effects on the kidney. The drug can cause changes in either the tubular or vascular system which may be either functional or structural, reversible or irreversible and frequent or seldom. Functional changes to the tubules and vessels are common, occur at low doses and are reversible, while structural changes to the tubules and vessels are rare, occur at high doses and are not always reversible. The changes of importance are vascular. By harnessing two strategies to restrain the development of side-effects--limiting the dose and restricting the functional changes--it is possible to avoid irreversible damage to the kidney in patients treated with CyA.
Cyclosporins↗
Indirect assessment of renal dysfunction in patients taking cyclosporin A for autoimmune diseases.
The reliability of increases in serum creatinine and serum urea above initial values in detecting decreases in glomerular filtration rate below initial values was assessed in patients taking cyclosporin A (CyA) for autoimmune diseases. Both serum creatinine and serum urea provided reasonable estimates of the decline in glomerular filtration rate when measured simultaneously and when compared to patients' own baseline values. Combination of both serum creatinine and serum urea improved the assessment of renal dysfunction, partly by reducing the influence of any analytical errors. Frequent measurements, precise baseline evaluation and standardization of procedures can be expected to increase further the precision of assessing renal dysfunction from serum measurements. Hence, careful evaluation of changes in serum creatinine, with the option to include changes in serum urea, can provide a simple and reliable way of monitoring changes in renal function in patients taking CyA for autoimmune diseases.
Autoimmune Diseases↗
Pharmacology of cyclosporine (sandimmune). I. Introduction.
Explore the source record for details and available documents.
Animals↗