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Biomedical subjects

J Marrink

Publications and source records attributed to J Marrink.

66 records · Page 4Linked to original sources
PubMed1979

A biclonal origin of two monoclonal proteins, IgG3(kappa) and IgA1(lambda), from a single patient.

The cellulose acetate electrophoretic pattern of the serum from patient Sik disclosed two distinct peaks, representing two monoclonal proteins. On immunoelectrophoresis the two M-components were found to differ in heavy chain class as well as in light chain type, IgG3(kappa) and IgA1(lambda). Serum immunoglobulin levels remained relatively constant over a period of 7 years and no clinical symptoms of a malignant deterioration occurred. It was found that the isolated M-components did not share idiotypic antigenicity. Bone marrow cells synthesizing the monoclonal proteins were identified by means of the immunofluorescent technique using isotypic as well as idiotypic antisera. Two distinct monoclonal cell populations were observed, containing either the IgG3(kappa) or the IgA1(lambda) monoclonal protein. The alpha 1-chain belonged to the VHIII subgroup, whereas the gamma 3-chain was found to be blocked. Subsequent sequence determination showed the gamma 3-chain to belong to the VHIII subgroup. It was concluded that the two M-components in the serum of patient Sik resulted from two independent neoplastic transformations.

Aged↗
PubMed1979

Treatment of renal amyloidosis with dimethylsulphoxide (DMSO).

Amyloid fibrils can be dissolved by dimethylsulphoxide (DMSO) in vitro and in vivo. This prompted us to investigate the therapeutic value of DMSO in renal failure caused by amyloidosis. Two patients with renal failure caused by secondary amyloidosis due to rheumatoid arthritis, showed a remarkable improvement of renal function. The effect of DMSO in amyloidosis secondary to rheumatoid arthritis seems to depend on its anti-inflammatory action, resulting in a decrease in amyloid formation. No evidence was found for an increase in amyloid degradation. The effect of DMSO in primary amyloidosis was inconclusive.

Amyloidosis↗
PubMed1977

Immunoglobulins in families of myeloma patients.

The serum immunoglobulin levels of 200 first-degree relatives and 23 spouses of 32 patients with myelomatosis were compared with those of age- and sex-matched control persons. First-degree relatives of myeloma patients appeared to have higher serum levels of IgG (P less than 0.05), IgA (P less than 0.05), and IgM (P less than 0.01) than their age- and sex-matched controls. The spouses as a group did not differ from their controls in this respect. No statistically significant difference in the incidence of M-components among family members (0.6%) and spouses (4.3%) was found compared with the incidence in the general population (0.9%). Myelomatosis as such appeared to be more frequent than in the general population (P less than 0.05).

Adolescent↗
PubMed1977

The clinical course of IgA nephropathy in adults.

Renal function was studied in 25 patients with IgA nephropathy, aged 15--48 years, during a mean follow-up period of 47 months. GFR remained normal in ten patients but decreased in 15, necessitating regular hemodialysis in five. The evolution of GFR was not related to the degree of increase of the serum IgA level or to the presence of recurrent respiratory tract infections. The mean age of the patients with decreased GFR was ten years older and the mean follow-up time 20 months longer than of patients with maintained renal function. Also these patients had more proteinuria and were more frequently hypertensive. Their initial renal biopsies showed histologic evidence of nephron loss. The hypothesis is discussed that IgA nephropathy in adults not infrequently follows a slowly progressive course.

Adolescent↗
PubMed1976

Immune response in asymptomatic paraproteinemia. I. Primary and secondary antibody response to Helix pomatia hemocyanin.

In a group of 20 patients with asymptomatic paraproteinemia, as judged after at least 3 years of follow-up, the primary and secondary antibody response to Helix pomatia hemocyanin (HPH) was defective as compared with the response in controls. The class of antibody was assessed by mercaptoethanol (ME) treatment of serum. A lowered response was found not only in the total but also in the ME-resistant (mainly 7S, IgG) antibody titer. Low anti-HPH antibody titers were preferentially found in the patients with high serum paraprotein levels, whereas in half of the patients with low serum paraprotein levels a completely normal antibody response was found. No differences in the total or 7S anti-HPH antibody response were found between patients with IgG, IgM, or IgA paraproteinemia. The polyclonal serum Ig levels were not predictive for the measured anti-HPH response. The anamnestic diphtheria and tetanus antibody response was not different from that of controls.

Adult↗
PubMed1968

Proteolytic activity at neutral pH in bovine spleen.

Proteolytic activity at neutral pH can be demonstrated in extracts from beef spleen. This activity is completely due to an aminopeptidase - or a number of aminopeptides - which is able to hydrolyse proteins completely to amino acids. No evidence was found for the presence of endopeptidases active at neutral pH. The enzyme resembles to some extent swine kidney aminopeptides.

Journal Article↗