5-Aza-2'-deoxycytidine which is preferentially taken up by the lymphatic tissue and is incorporated into DNA strongly affects the ability of the immune system to synthesize IgG antibodies against sheep red blood cells without affecting IgM antibody formation. A single dose of the drug results in a prolonged inhibition persisting 14 days after the secondary immunization. The inhibitory effect is dose-dependent and is maximal when 5-aza-2'-deoxycytidine is given 2 days after sheep erythrocytes. The drug affects the utilization of thymidine for the synthesis of DNA in the spleen; under certain conditions the enhancement of the rate of DNA synthesis in the spleen has been observed.

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PubMedDate not supplied

Age-dependent rapid antiinsulin effect of hydrocortisone hemisuccinate during insulin induced hypoglycemia in white rat.

The effect of hydrocortisone hemisuccinate upon the intravenous insulin-induced hypoglycemia in various age-groups of white rats was studied. Insulin was injected intravenously in a dose of 10-(2) units per 100 g b.w. in the same age-groups of animals in a basal state, and 2 days later, 15 minutes after the i.p. administration of hydrocortisone hemisuccinate (0.5 mg/100 g b.w.). It has been estabished, that hydrocortisone hemisuccinate had a rapid anti-insulin effect, which is quantitatively different in dependence on the age of animals.

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PubMedDate not supplied

Insulin effect upon the "in vitro" glucose uptake by the white rat thymus during the age - and hydrocortisone - induced thymus involution.

Insulin at a physiological concentration (10(-3) IU/ml incubation medium) did not modify the glucose uptake by the thymuses of young rats, but increased significantly the glucose uptake by the involuted thymuses of older animals. Insulin at the same concentration enhanced the glucose uptake of the thymuses which underwent an accidental hydrocortisone-induced involution. Parallely, the effect of insulin on insulin dependent peripheral tissues (diaphragm and epididymal fat pad) was followed. In the presence of cystine (4.13 mumole/ml) in the incubation medium increased the glucose consumption by the thymus of young rats. This fact is discussed on the basis of the -S-S bond-protecting effect of cystine against the distroying effect of free -SH groups released from the thymus into the incubation medium.

Age Factors↗

PubMedDate not supplied

Effect of hydrocortisone hemisuccinate on the level of blood inorganic phosphorus, on the glucose assimilation coefficient and on the rate of 32P disappearance from the blood, in white rats.

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Animals↗