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Biomedical subjects

J M Riley

Publications and source records attributed to J M Riley.

27 records · Page 2Linked to original sources
PubMed1967

Effect of virus input multiplicity and tissue cell concentration on growth of Rift Valley fever virus.

The effects of virus input multiplicity and of tissue cell concentration upon the growth of Rift Valley fever virus in L cells (Earle) were determined. The titers obtained in suspension cultures with cells obtained from two separate laboratories were significantly different. With both monolayer culture and suspension culture systems, a virus input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yields were obtained in suspension cultures containing 4 x 10(5) tissue cells per ml of suspension.

Arboviruses↗
PubMed1967

Application of the single-dose assay technique to psittacosis.

When suspensions of psittacosis organisms were injected intracerebrally into groups of mice, a nearly linear relationship was observed between the concentration of the organisms injected and the mean time to death of the mice. Thirty-four psittacosis preparations were assayed and, by plotting the relationship between the reciprocal time to death for mice given the 10(-3) dilution of agent against the number of mouse intracerebral median lethal doses (MICLD(50)), a reference curve was established. By use of this reference curve, it was possible to estimate directly the LD(50) value of a psittacosis suspension of unknown concentration from the mean reciprocal time to death of a group of mice injected with a single dilution. By this procedure, the number of mice used was reduced by 62.5%, the titrations were completed in 3 to 5 days as compared with the usual 12 days, three to four times as many assays could be done in 1 day, and no assays had to be repeated. In addition, the precision of the single-dilution assay compared favorably with that of the LD(50) titration.

Animals↗
PubMedDate not supplied

Preliminary evaluation of two experimental surgical adhesives in the rabbit cornea.

We examined the clinical and histopathologic effects of the instillation of two surgical adhesives in the corneal stroma of rabbits. The adhesives examined in this study included a butyl-2-cyanoacrylate (CA) and a combination of a mussel-derived polyphenolic protein (MAP) with an enzyme polymerizer (COX). In this study, either one of the adhesives or balanced saline solution (as a control) was instilled directly into the corneal stroma following the surgical creation of a perilimbal intralamellar pocket. Animals were examined on a regular basis for a 60-day period following instillation and, at predetermined time points, sacrificed for histopathologic evaluation. The group of animals that received intracorneal instillations of CA consistently manifested early and progressive stromal neovascularization of the affected corneas. Histopathologic examination confirmed the progressive neovascularization, as well as a noticeable inflammatory response surrounding the adhesive. In contrast, instillation of the MAP-COX adhesive produced neovascular and inflammatory responses that were later in onset and milder in degree than those observed with CA. In this experimental model, the MAP-COX adhesive appeared to be better tolerated than did the CA.

Animals↗