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Biomedical subjects

J M Rigot

Publications and source records attributed to J M Rigot.

At least 37 records · Page 2Linked to original sources
PubMed1996

Congenital bilateral absence of the vas deferens (CBAVD) and cystic fibrosis transmembrane regulator (CFTR): correlation between genotype and phenotype.

To assess better the link between congenital bilateral absence of the vas deferens (CBAVD) and cystic fibrosis (CF), we compared sweat chloride values, analysis of the CFTR intron 8 poly(T) tract length and analysis of 10 exons in a population of 38 patients with CBAVD. The data indicate that this population can be divided into three groups of patients. In the first group of 15 patients with abnormal sweat chloride (> 60 mmol/l), the frequency of CF mutations is high. In the second group of 18 patients with equivocal sweat chloride (between 40 and 60 mmol/l), the frequency of the 5T variant is high; 6 patients have a delta F508 mutation and a 5T variant and 1 patient is homozygous for the 5T variant; a 5T variant has been detected in 3 other patients, and a delta F508 mutation in another patient. A third group of 5 patients is probably not related to CF: these patients have other congenital abnormalities of the urogenital tract, low chloride values (< 40 mmol/l) and apparently no abnormality of the CF gene.

Adult↗
PubMed1996

Molecular mapping of a Yq deletion in a patient with normal stature.

The proximal long arm of the Y chromosome probably contains a gene (GCY) involved in stature determination. Recent reports have proposed the critical region extends from interval 4B to interval 5G (or 5E). In the present study, the deletion breakpoint in a male adult patient of normal height with a 46,X,del(Yq) karyotype was defined by the use of sequence-tagged site markers. The breakpoint was found between sY78 (interval 4B) and sY79 (interval 5A). The existence of a normal stature in this patient suggests that the growth determinant is proximal to sY79, therefore probably located in interval 4B or in proximal interval 5A of the Y chromosome.

Adult↗
PubMed1996

Delayed vasal reanastomosis in rats: comparison of a microsurgical technique and a fibrin-glued procedure.

OBJECTIVES: To compare fibrin-glued vasovasostomy to a conventional microsurgical technique in a protocol of delayed vasovasostomy. MATERIALS AND METHODS: Forty male Sprague-Dawley rats underwent bilateral vasectomy through a midline abdominal incision. Two weeks later all animals underwent a bilateral vasectomy reversal through a bilateral inguino-scrotal incision, following two different protocols. Invariably, the proximal segment had a larger lumen. The control group (20 rats) had a conventional modified one-layer sutured vasal anastomosis with 10/0 nylon. The experimental group (20 rats) underwent vasal anastomosis using fibrin glue and consisting of three transmural sutures with 10/0 nylon followed by the application of fibrin glue circumferentially to seal the anastomosis. The fibrin-tissue adhesive was obtained from pooled donor plasma and was virally inactivated by a solvent-detergent treatment. Seven weeks after surgery all animals were killed and the vasal specimens were evaluated for gross patency and the incidence of sperm granuloma. RESULTS: The control group had a patency rate of 85% and half had sperm granuloma. The experimental group had a patency rate of 92% and 40% had sperm granuloma; neither difference was significant. The mean operative time was significantly shorter for the fibrin glue-assisted vasovasostomy (P < 0.001). CONCLUSION: This study showed that a delayed fibrin-glued vasovasostomy gave a comparable anatomical success and an incidence of sperm granuloma similar to that using a conventional microsurgical technique, but with the advantages of a shorter operative time and a less technically demanding anastomosis.

Anastomosis, Surgical↗
PubMed1996

[Malignant mesothelioma of the testicular tunica vaginalis].

Malignant mesothelioma of the tunica vaginalis, a rare tumour, affects men over the age of 50 years and presents in the form of a testicular mass. It is rarely suspected preoperatively and the diagnosis is suggested by the presence of numerous nodules in the tunica vaginalis. This tumour has a high metastatic potential and raises the problem of numerous local recurrences. The diagnosis is histological with negative biological markers. Treatment is difficult; the first step must consist of surgery. The indications for radiotherapy have yet to be defined.

Aged↗
PubMed1996

Synaptic behaviour of sex chromosome in two XYY men.

Analysis of meiotic pairing behavior in two XYY males presenting at subfertility clinic brings evidence that the abnormal mode of pairing caused by the presence of the extra Y chromosome, disturbs achievement of spermatogenesis resulting in severe oligoastheno-teratozoospermia. In contrast, the loss of the supernumerary Y before meiosis, allows achievement and normal sperm production.

Adult↗
PubMed1995

[Report of a case of penile metastasis. Review of the literature].

Penile metastases are rare. They occur an average of 2 years after diagnosis of the primary tumour and are frequently associated with disseminated metastases. Treatment is more often palliative than curative, allowing a survival of 2 years. The authors report a case of penile metastasis secondary to a rectal cancer, discovered 29 months after treatment of the primary site. Survival with chemotherapy was 15 months.

Adenocarcinoma↗
PubMed1994

Abnormal expression of protein 4.1 in spermatozoa of infertile men with teratospermia.

Molecular defects responsible for morphologically abnormal spermatozoa (teratospermia) associated with male sterility are largely unknown. We report defective expression of protein 4.1, a cytoskeletal protein initially recognised in red cells. In some patients with severely amorphous sperm heads, protein 4.1 had an abnormal sub-cellular localisation (tail instead of head) and appeared as high-molecular-weight isoforms, especially a 135 kDa species instead of the 82 kDa isoform seen in fertile sperm. Because the 135 kDa isoform is characteristic of immature germ cells from testis, its presence in teratospermia suggests profoundly disturbed sperm differentiation.

Cytoskeletal Proteins↗
PubMed1994

Evidence for eosinophil activation in eosinophilic cystitis.

Eosinophilic cystitis (EC) is a rare condition. Recent studies have shown that activated eosinophils release cytotoxic cationic proteins which can induce tissue damage. Moreover, in vitro studies have shown that interleukin-5 (IL-5) is a cytokine able to attract and activate eosinophils. The goal of this study was to detect a possible activation of eosinophils in EC using electron microscopy, in situ hybridization with an IL-5 RNA probe and immunochemistry with a specific anti-IL-5 antibody. Using these combined methods in a typical case of EC, we found numerous activated eosinophils synthesizing and secreting IL-5 protein. IL-5 could enhance the activation of eosinophils and their cytotoxic potential in bladder tissues. This mechanism might explain the chronicity of the lesions in EC.

Cystitis↗
PubMed1994

MR findings of ejaculatory duct cysts.

Ejaculatory duct cysts are a rare type of prostatic cysts. We report 3 cases of symptomatic ejaculatory duct cysts which have been explored by MR imaging. The MR findings were round or oval masses, medial or paramedial in the prostatic gland above the level of the verumontanum, extending into the prostatic base. They displayed a low signal intensity on T1-weighted images and high signal intensity on T2-weighted images (2 cases) or high signal intensity on both T1- and T2-weighted images (1 case). The diagnosis was confirmed by an ultrasonographically guided transperineal aspiration demonstrating spermatozoa in the cyst fluid.

Adult↗
PubMed1993

[A priori benign Leydig cell testicular tumor: enucleation or radical orchidectomy?].

The histological sections of 5 patients treated for clinically benign Leydig cell testicular tumour (LCTT) were reviewed according to histological criteria presumptive of malignancy. The absence of any sign of severity was only verified in a single case, as none of the other tumours could be considered to be strictly benign. In the light of these findings, great caution is required in conservative indications, especially as these tumours, when they are malignant, are particularly serious due to the inevitable diagnostic delay and their absence of radio- or chemo-sensitivity.

Adult↗