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Biomedical subjects

J M Montgomery

Publications and source records attributed to J M Montgomery.

23 records · Page 2Linked to original sources
PubMed1979

Effects of postural drainage, exercise, and cough on mucus clearance in chronic bronchitis.

The effects of postural drainage, exercise, and cough on mucus clearance were compared in 8 patients with chronic bronchitis. A bolus of 99mTc albumin aerosol was inhaled at a high inspiratory flow rate to enhance proximal deposition. Retention of deposited aerosol in the lung, as a function of time, was quantified using a gamma camera and subsequent computer analysis. Coughing greatly accelerated total lung (P less than 0.005) and peripheral (P less than 0.005) mucus clearance. Exercise resulted in much smaller changes than did cough, but significantly increased total lung clearance (P less than 0.005). Postural drainage in which coughing was prohibited did not alter clearance. These results have therapeutic implications and stress the importance of controlling cough when evaluating therapeutic interventions by these techniques.

Aged↗
PubMedDate not supplied

Development of an immunomagnetic antigen capture system for detecting leptospires in bovine urine.

A magnetic bead antigen capture system which combined the use of two evolving techniques - immunomagnetic separation (IMS) and time-resolved fluoroimmunoassay (TR-FIA) - was developed to detect Leptospira borgpetersenii serovar hardjo in bovine urine. The assay utilised monoclonal antibody coated magnetic beads to capture leptospiral antigen which was in turn detected using another monoclonal antibody (Indicator) labelled with biotin. Signal was generated by the binding of europium labelled streptavidin to indicator antibody. The sensitivity of the assay was improved from 10(3) to 10(2) leptospires per ml by using an ethanol precipitation procedure to treat each sample. The assay detected only 31 of 56 (55 per cent) urine specimens culture-positive for hardjo, but seven of 24 urine samples culture-negative for hardjo were identified as positive by the assay. These seven samples were from animals which were culture positive on at least one other occasion. These results suggest that this system should be further investigated as a complementary test to culture for the identification of hardjo carrier animals.

Animals↗
PubMedDate not supplied

Detection of Streptococcus pneumoniae and Haemophilus influenzae type b antigens in the serum and urine of patients with pneumonia in Papua New Guinea: comparison of latex agglutination and counterimmunoelectrophoresis.

Latex agglutination (LA) was compared with counterimmunoelectrophoresis (CIE) for the diagnosis of pneumonia due to Streptococcus pneumoniae or Haemophilus influenzae type b in children less than 6 years old in Papua New Guinea. Neither LA nor CIE was sufficiently sensitive for the detection of pneumococcal antigens. CIE was superior to LA but had a sensitivity of only 60% (six of 10) in concentrated urine and a specificity of 90% (54 of 60). LA for the detection of H. influenzae type b had a sensitivity of 100% (eight of eight) relative to that of blood culture and a specificity of 99% (112 of 113) when urine diluted fivefold was tested, as compared with a sensitivity of 100% (nine of nine) and a specificity of 92% (56 of 61) in undiluted urine. Undiluted and concentrated urine specimens were unsuitable for LA because of the many false-positive reactions that resulted from nasal carriage of H. influenzae type b and nasal carriage of the cross-reactive S. pneumoniae type 6. CIE for the detection of H. influenzae type b had a sensitivity of only 44% (four of nine) in concentrated urine.

Antigens, Bacterial↗
PubMedDate not supplied

Bacterial colonization of the upper respiratory tract and its association with acute lower respiratory tract infections in Highland children of Papua New Guinea.

Acute lower respiratory tract infection (ALRI) is the major cause of death among children in Papua New Guinea. This longitudinal study reports the bacteriologic findings for children observed in their hamlets. A total of 1,449 nasal swab specimens from 158 children less than 5 years of age who were studied intensively for 18 months were examined. Non-serotypable strains of Haemophilus influenzae were isolated from 91% of specimens, and serotypable strains were isolated from 35% (8% H. influenzae type b) of specimens. All children had acquired Streptococcus pneumoniae by the age of 3 months. The most frequently occurring serotypes of S. pneumoniae were 6, 19, and 23. Children more frequently carried invasive pneumococci during an episode of ALRI than when they were healthy. Also, children more frequently carried serotypable strains of H. influenzae during the 2 weeks preceding an episode of ALRI than when they were healthy. Between-children analyses showed that children who were susceptible to attacks of ALRI and those who were not susceptible had similar rates of carriage of bacteria.

Age Factors↗