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Biomedical subjects

J M Kyd

Publications and source records attributed to J M Kyd.

22 records · Page 2Linked to original sources
PubMed1992

Deciduogenic effects of mediators of the polyphosphatidylinositol pathway in pseudopregnant mice.

Intraluminal injections (15 microliters) of either concanavalin A (125 micrograms) or ionophore A 23,187 (0.01 mumol) induced a decidual cell reaction (DCR) in the uterus of day 4.5 pseudopregnant mice. However, when these agents were administered in different combinations with each other or with CaCl2 (15 mumol) and phorbol-12-myristate-13-acetate (1.6 nmol), interacting effects occurred to either enhance or inhibit each of the others' independent deciduogenic capacities. The results suggest that the polyphosphatidylinositol pathway and Ca2+ are involved in the induction of the DCR in mice with complex interactions occurring between the active components of the pathway to modulate the outcome of the transformation process.

Animals↗
PubMed1980

Adenosine kinase from human erythrocytes: determination of the conditions required for assay in crude hemolystates.

An assay suitable for initial velocity studies was developed to measure adenosine kinase (ATP: adenosine 5'-phosphotransferase, EC 2.7.1.20) activity in crude lystaes of human erthrocytes. The pH optimum for the reaction was dependent on the ratio of Mg2+/ATP used in the assay. With a Mg2+/ATP ratio of 5.0, the pH optimum was 5.1 and only 10% of the maximal activity was retained at pH 7.3. When a Mg2+/ATP ratio of 0.50 was used, the pH optimum was 6.2, but 70% of the maximal activity was retained at pH 7.3 For assays performed at pH 7.3, the optimal Mg2+/ATP ratio was 0.50 for ATP concentrations between 0.50 and 2.0 mmol/l. Mg2+ was required for reaction, presumably to form the physiological substrate MgATP2-. However, excess free (uncomplexed) Mg2+ was a strong inhibitor of the enzyme at pH 7.3. At pH 7.3, the Km values for adenosine and MgATP2- were 0.66 mu mol/l and 82 mu mol/l respectively.

Adenosine↗
PubMedDate not supplied

Viral co-infection does not reduce the efficacy of vaccination against non-typeable Haemophilus influenzae middle ear infection in a rat model.

The mucosal vaccination of rodents with killed non-typeable Haemophilus influenzae (NTHi) has been previously shown to enhance live NTHi clearance following middle ear challenge. This study assessed the efficacy of mucosal anti-NTHi vaccination during a concomitant viral infection of the respiratory tract. Animals were mucosally immunised with killed NTHi by intra-Peyer's patch primary inoculation and lung (intratracheal) boost. At the time of both immunisations rats were also infected intra-nasally with Sendai virus. Concomitant Sendai virus infection did not influence the efficacy of anti-NTHi vaccination mediated clearance of NTHi from the middle ear. This would suggest that immunisation strategies to prevent bacterial middle ear infection would be effective despite the presence of concomitant viral agents.

Acute Disease↗