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Biomedical subjects

J M Joseph

Publications and source records attributed to J M Joseph.

At least 73 records · Page 4Linked to original sources
PubMed1977

Asymptomatic females: detection of antibody activity to gonococcal pili antigen by radioimmunoassay.

A gonococcal pili antigen preparation was used to detect antibody activity sera obtained from 322 culture-positive asymptomatic females and 150 negative controls. Pili were obtained from a culture of type 2 Neisseria gonorrhoeae (strain 2686) and labeled with 125I for use in a double-antibody radioimmunoassay test system. Of the 322 sera obtained from culture-positive, asymptomatic females, 276 (85.7%) showed antibody activity greater than or equal to 1.8 mug/ml. Negative controls were obtained from three different groups of individuals, and 130 (86.7%) had undetectable antibody activity. Sera from asymptomatic, culture-positive females were absorbed with three different strains of N. gonorrhoeae, one of these strains being the organism used for pili antigen preparations. The absorbed sera were tested for antibody activity, and in each case the activity in the absorbed sera dropped to an undetectable level. When the same sera were absorbed with N. meningitidis, N. catarrhalis, N. perflava, Escherichia coli, Herellea vaginicola, Mima polymorpha, Staphylococcus aureus, and Candida albicans, little, if any, decline in the level of anti-pili antibody activity was observed.

Animals↗
PubMed1976

Antibody response in patients with acute nonlymphocytic leukemia.

Lymphocytotoxic (LCT) and anti-red blood cell (ABO) antibodies were measured serially in adult patients with acute nonlymphocytic leukemia (ANLL) receiving induction chemotherapy. The antigenic stimulus was provided by multiple platelet transfusions, many of which were ABO incompatible. Comparison of pretherapy titers 4-6 weeks into therapy shows that 50% (19/38) of patients became LCT positive (cytotoxicity against greater than 10% of panel of cells) and 54% (19/35) had increases in ABO titers (greater than 2 tube dilution). A total of 66% of patients had significant rises in at least one antibody. ABO and LCT titers tended to vary in parallel although exceptions were noted. The development of anti) remission rate or duration, 3) type of therapy, 4) number of platelet transfusions, 5) time relationship between the antigenic stimulus and the initiation of cytotoxic therapy, and 6) skin test reactivity. Antibody responders tended to have higher pretreatment lymphocyte counts. The ability to develop a secondary antibody response does not appear to be a major prognostic factor in ANLL.

ABO Blood-Group System↗
PubMed1976

Person perception and the Berkowitz paradigm for the study of aggression.

Three experiments in person perception were conducted to investigate the conditions under which naive observers label an actor as aggressive and to ascertain how this label affects the reactions of the observers to the actor. These studies were performed by exposing subjects to written descriptions of Berkowitz's much-used paradigm for the study of aggression in order to additionally demonstrate that naive observers do not label in the same way as do research psychologists. In Experiment 1 the hypothesis was confirmed that harm-doing behavior is labeled as aggression only when it is antinormative. When harm doing was justified by the instigation or attack of another person, its use did not lead to the actor being labeled as aggressive. In Experiment 2 it was found that violation of the limits imposed by the experimenter on the number of shocks that could be used by actors led observers to characterize them as aggressive even when their action was justified by the prior instigation of the other party. Violation of two norms led to a more aggressive rating of the actor than did the violation of only one norm. Experiment 3 showed that actors who are labeled as aggressive are disapproved of and avoided by observers. The results of these studies are discussed in terms of the ecological validity of Berkowitz's research paradigm and metatheoretical implications for the scientific study of harm-doing behavior.

Aggression↗