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Biomedical subjects

J M Hicks

Publications and source records attributed to J M Hicks.

60 records · Page 4Linked to original sources
PubMedDate not supplied

Is heparinized plasma suitable for use in routine biochemistry?

Using lithium heparin plasma and serum, we compared the values of 27 biochemical analyses performed with a Kodak Ektachem 500 analyzer. For 16 of the 27 tests, we observed statistical differences between the mean results obtained from the analysis of heparinized plasma and those obtained from the analysis of serum (Student t-test; P < .001). However, none of these differences were medically significant. When the concentration of lithium heparin was increased to three and five times normal (reflecting incomplete Vacutainer tube filling), alanine aminotransferase, amylase, aspartate aminotransferase, lipase, and potassium all exhibited some changes compared with serum. Therefore, heparin may be used to shorten the turnaround time in the routine biochemistry laboratory, but the anticoagulant tubes should be completely filled to prevent spurious intraindividual variations in serial specimen analysis.

Adult↗
PubMedDate not supplied

Stability of twenty-five analytes in human serum at 22 degrees C, 4 degrees C, and -20 degrees C.

The stability of 25 analytes from serum of healthy donors was determined at room temperature, 4 degrees C, and -20 degrees C over 48 h, 14 days, and 4 months, respectively. Glucose, blood urea nitrogen, sodium, potassium, chloride, creatinine, calcium, phosphorus, uric acid, total protein, albumin, triglycerides, lipase, total creatine kinase, gamma-glutamyltransferase, iron, magnesium, and cholesterol were stable at all three temperatures for the specified times. Carbon dioxide, aspartate and alanine aminotransferases, lactate dehydrogenase, amylase, alkaline phosphatase, and high-density lipoprotein cholesterol demonstrated some loss over time. Proper storage temperatures and times must be considered for these analytes if measurement is not to take place immediately after specimen collection.

Adult↗