Search PubMed⌕ Search

Biomedical subjects

J M Doby

Publications and source records attributed to J M Doby.

At least 19 recordsLinked to original sources
PubMed1995

[Risk of contamination by Borrelia burgdorferi S. lato in a forest environment. Survey during 13 months of abundance of the tick Ixodes ricinus and its level of infestation by the Lyme borreliosis agent in Brittany].

The authors, in a forest in Brittany previously studied for several years, caught by flagging, each month from April 1992 to May 1993, nymphs of I. ricinus tick, and looked by indirect immunofluorescence, for B. burgdorferi infestation. An amount of 1,506 ticks was thus studied. Infestation frequency was varying from 0 per cent in January and February to 14.4 per cent in August. Standarding of tick collecting method allowed to establish, for each month, a tick, "availability" index, and, according to the spirochete infestation frequency, to do estimation of the risk level, for human visiting the concerned forest, of being infected by B. burgdorferi. Obtained results show that this risk is the highest in August, and quite non-existent in January and February.

Animals↗
PubMed1992

[Survey of the infestation level of the Ixodes ricinus tick by Borrelia burgdorferi. Complimentary report].

In a recent report, we gave results of a 4 year consecutively survey (1987 to 1990) of the level of Ixodes ricinus nymphs infested by Borrelia burgdorferi in two forests of the western part of France. This brought us to conclude that there was a relative stability for this level in course of time. Results of this survey kept on in 1991 oblige us to partly modify our conclusion, on account of an important dropping of the tick infection frequency in one of the two forests, this perhaps in connection with a large observed decreasing of the micromammals populations (but not of ticks ones) due probably to an exceptional drought during all the 1990 year.

Animals↗
PubMed1991

[Survey during 4 years of the infestation level of the tick Ixodes ricinus (acari Ixodidae) by Borrelia burgdorferi, the agent of Lyme borreliosis, in 2 forests in Brittany].

The authors followed, during 4 years consecutively, from 1987 to 1990, by immunofluorescence, the frequency of B. burgdorferi in an amount of 677 nymphs of I. ricinus tick, collected fasting by flagging in 2 forests in Brittany (France). Percentages obtained in each of these forests do not reveal significative differencies statistically and seem to show a relative stability, from one year to the following, during the considered period, of the infestation levels in ticks.

Animals↗
PubMed1991

[Tick spirochetosis by Borrelia burgdorferi in wild carnivores in France. Results of serologic tests in 372 foxes].

The authors made serological examination, by passive hemagglutination, of 378 wild carnivores killed in 17 departments of western, central and eastern parts of France: 372 of foxes, of which more than 90 per cent less than one year aged, 4 raccoon dogs and 2 lynx. In foxes, they found significative seropositivity (greater than or equal to 1/200) with mean frequencies of 12.77 per cent in young animals and 42.86 % in those of 1 year and more. According the parts of France, frequency is greatly varying, being highest in the department of Ain, in Jura. The frequency of antibodies against B. burgdorferi in foxes brings the authors to think that it is necessary to define precisely their importance as reservoirs for this infection.

Age Factors↗
PubMed1987

Lyme disease in Canada with possible transmission by an insect.

The first documented case of Lyme disease to have originated in Canada is presented. A 51-year old French female nurse visited Quebec, Canada from 20 July to 5 August, 1984 and contracted Lyme disease. She reported an insect bite on 3 August 1984 on the back of her knee with erythema chronicum migrans appearing at the site of the bite on 5 August 1984. The patient presented with meningeal lymphocytic reaction near the end of September, 1984. Total Ig serum titers in indirect immunofluorescence tests on 1 October 1984, 10 October 1984, and 10 January 1985 were 1/512, 1/2048 and 1/16,384, respectively.

Animals↗
PubMed1987

[Comparative value of immunoelectrophoresis and ELISA in the serological diagnosis of human hypodermyiasis].

186 sera of patients with hypodermatosis were comparatively studied by immunoelectrophoresis and ELISA. After establishing limits of certainty (2 distinct precipitating arcs with IEP and an optical density greater than or equal to 0.8 with ELISA), the comparison of obtained results shows that there is a good concordance between the two methods, but that the second, by its higher sensitiveness, allows to reveal residual antibodies during a delay after disease recovery longer than with the first. Consequently, this higher sensitiveness allows, first, retrospective diagnosis, then the more frequent settling, when border-lined serological positivities are obtained with IEP, therefore without possible conclusions. On the other side, importance of crossed reactions between Hypoderma bovis and H. lineatum revealed with ELISA, does not allow, contrarily to what is done frequently by IEP, much more specific method, to know the species of Hypoderma causing the disease and, therefore, to bring more to the prognosis.

Enzyme-Linked Immunosorbent Assay↗
PubMed1985

[Human hypodermyiasis in France in 1984: 266 cases recorded to date. Distribution according to known geographic origin].

The authors, after collecting all human hypodermosis cases in France they know to this day, to the number of 266 (cases in the literature, and unpublished, diagnosed or by them, in the great majority, or by others), make the situation, at the beginning of 1984, of this parasitosis and do comments on the evolution in time of the number of cases according to the geographical regions in France.

France↗
PubMed1985

[Etiologic and epidemiologic questions posed by erythema chronicum migrans and Lyme disease. Apropos of 4 cases at the Regional Hospital Center, Rennes].

The authors give 4 observations, made in Rennes (France), of 3 cases contracted in France and 1 in Canada, cases they think to be related, for 3 at least, to the infectious entity described by American authors under the name of Lyme disease. If 1 of these cases was limited to an erythema chronicum migrans, the 3 others showed a beginning evolution towards a classical Lyme disease. In 2 of these 3 last cases, the infection was perhaps cut short by a quickly applied therapy by antibiotics. They discuss the different hypotheses about the negative Borrelia serologic reactions in 3 of the 4 cases. A possible arboviral aetiology is evocked. In only one of the 4 cases, a tick-bite can be asserted, the role in the the transmission of this kind of arthropods being strictly excluded in 2 of the 3 other cases (transmission likely by a mosquito and by a biting fly (tabanid?)). The authors put the question of the possibility of transmission, for erythema chronicum migrans and Lyme disease, by arthropods other than ticks.

Adult↗
PubMed1983

[Possibilities of obtaining, by passage on plantlets, reproduction forms (porospores) in strains of Alternaria (dematiaceous fungi) isolated from human cutaneous lesions and having lost the possibility of fructifying. Preliminary results].

Strains of Alternaria (Dematiaceous Hyphomycetes) isolated from lesions in men or animals generally lose quickly their ability to fructify (porospores forming) after their isolation and keeping up on artificial culture mediums classicaly used in medical diagnostic laboratories. The authors, from the fact that Alternaria are fungi which are normally parasites of living plants, put, on plantlets from germinating corn seeds (graminaceous plant) and lentil seeds (papilionaceous plant), two strains of Alternaria isolated from human cases of alternariosis which were diagnosed in their laboratory and which did not give porospores since delays reaching 14 months. They obtained, on corn plantlets, very numerous, normal and mature porospores, and on lentil plantlets, or nothing with the first strain, or very rare porospores with the second. This method, if results are confirmed by further experimentations, will be usefull for the specific determination for strains isolated from lesions and become infertile. The differences of behaviour of two strains, according the botanical family of plantlets used, come perhaps from the relative host specificity of the most numerous species of Alternaria.

Alternaria↗
PubMed1982

[Respective frequency of Hypoderma species (Insecta Diptera Oestroidea) as the cause of human cases of hypodermiasis in France].

The authors, taking into consideration, on the one hand, numbers of larvae issued from human cases and respectively identified as Hypoderma bovis and H. lineatum (at least when the specific determination cannot be put in doubt), on the other hand, the comparative intensity of serological reactions obtained between sera of more than one hundred cases by them diagnosed, and antigens respectively prepared from H. bovis and H. lineatum first instar larvae, come to the conclusion that, in France at least, the most numerous cases have as agent the first of these two species. They discuss different hypothesis giving possible explanation of the observed differences of frequency. On account of this higher frequency of cases by H. bovis, and of the complete lacking, sometimes, of crossed reactions with H. lineatum, they think that, for all serological researchs of human hypodermatosis, antigens corresponding to the two species have to be used, when, nearly always, alone is used H. lineatum antigen, for reason of easiness in collecting larvae for its preparing.

Diptera↗
PubMed1978

[Presence of sexual forms (cleistothecia and Hülle cells) in a case of human maxillary sinus aspergillosis caused by Aspergillus nidulans in association with Aspergillus fumigatus].

The authors observed, in a biopsic sample from a human maxillary sinus, the coexistence, in parasitic stages, of two different Aspergillus species: A. fumigatus and A. nidulans. After having described the morphologies of each species, in sections on slides, and particularly those of cleistothecia and Hülle-cells for the second, they draw attention to the three interests of such an observation: -- rarety of observing Aspergillus nidulans in a parasitic stage, in spite of its particular thermotolerant character. -- association of two different Aspergillus species in a same sample, in parasitic stages, observed in man for the first time, at least according their informations. -- presence of reproductive sexual forms, observed in vivo for the first time, in an Aspergillus species.

Adult↗
PubMed1976

[Attempt to obtain in vitro pre-erythrocyte forms of Plasmodium vivax in human liver cell cultures inoculated with sporozoites].

Using sporozoites of a strain of Plasmodium vivax of North Korean origin maintained in human subjects and in Anopheles atroparvus, the authors inoculated human liver cell cultures (primary cultures and Ist subcultures). The appearance of some rare intracytoplasmic forms is described, which are thought to be very likely connected with the pre-erythrocytic cycle of P. vivax. Further attempts are nevertheless necessary to confirm, or invalidate, the obtained results.

Animals↗