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Biomedical subjects

J M Cunningham

Publications and source records attributed to J M Cunningham.

154 records · Page 9Linked to original sources
PubMed1983

Combination of multiple-family therapy with parallel groups.

Parallel groups for children, adolescents, and parents have been a usual modality in the Community Guidance Center for over 12 years. This has provided effective therapeutic intervention. However, an inherent difficulty in these groups was discussion with parents about the identified patient's progress. Various methods, on a trial-and-error basis, were attempted. The most successful process, which evolved four years ago, is a multiple-family-therapy session combined with the parallel groups. The multiple-family-therapy group provides an interface for five subsystems [parents and children, also forming families; trainees; staff], opens communication to parents about the child's progress and includes the parent more viably in the treatment process. It provides greater awareness and opportunity for work toward family and individual goals with major focus on communication skills, role modeling for parents and trainees, and realistic goal setting.

Adult↗
PubMedDate not supplied

A stable long-term hepatocyte culture system for studies of physiologic processes: cytokine stimulation of the acute phase response in rat and human hepatocytes.

Prior studies on the in vitro hepatic acute phase response have involved either hepatoma cell lines or conventional short-term cultures of primary hepatocytes. No data are available on the response of primary hepatocytes in stable long-term culture systems. In this study, the acute phase response of rat and human hepatocytes in a new long-term culture system was examined in response to interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), and tumor necrosis factor alpha (TNF-alpha). The cultured cells were sandwiched between two layers of collagen in a (double-gel) configuration which has been shown to preserve both hepatocyte function and morphology over prolonged periods of time. The stability of this culture configuration enabled us to investigate, for the first time, the temporal aspects of the response in addition to the effects of the mediators on protein secretion. Exposure of rat hepatocytes to IL-6 after culture for 16 days resulted in a 2-fold reduction of albumin secretion and a 15-fold increase in the secretion rates of fibrinogen and alpha 2-macroglobulin. In all instances, the peak response occurred at 48 h after IL-6 exposure, and all protein secretion rates returned to pretreatment values within 5 days posttreatment. Changes in the mRNA levels of these proteins in response to IL-6 corresponded with those changes seen with the secreted products, indicating pretranslational regulation. Administration of IL-1 beta to rat hepatocyte produced a similar decline of albumin secretion and a 5-fold increase of fibrinogen secretion, whereas alpha 2-macroglobulin secretion remained undisturbed.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute-Phase Reaction↗
PubMedDate not supplied

Detection of p53 antigen expression in cytologic preparations of ovarian carcinomas.

We explored the value of p53 immunostaining in detection of malignant cells in cytologic analyses in ovarian cancer patients. Ninety-six percent of 23 tumor touch preparations had nuclear staining with PAb240: 44% had nuclear only, 26% had both nuclear and cytoplasmic staining, and 26% had cytoplasmic staining alone. Eight of 20 cytospins of peritoneal washings from ovarian cancer patients were PAb240 positive, 6 had cytoplasmic, and two had nuclear staining. Cytospins of fluid from 3/15 patients with no malignancy revealed cytoplasmic staining of mesothelial cells. The lack of concordance between staining of primary tumors and cytospins of peritoneal washings and the apparent reactivity in some benign samples may limit the utility of immunohistochemical detection of malignant cells in the peritoneal fluid of ovarian cancer patients.

Adolescent↗