Biomedical subjects

J Losy

Publications and source records attributed to J Losy.

46 records · Page 3Linked to original sources

PubMedDate not supplied

[Oligoclonal antibodies in the diagnosis of multiple sclerosis and other diseases of the nervous system].

The detection of CSF oligoclonal IgG is one of the most useful laboratory tests to aid in the diagnosis of multiple sclerosis. Oligoclonal IgG is synthesized within the CNS and is usually detected in the CSF by agar and agarose gel electrophoresis or by isoelectric focusing. Oligoclonal IgG is present in about 90-95% in patients with clinically definite MS. There is a predominance of kappa light chains and IgG1 and IgG3 subclasses in oligoclonal IgG in MS. The specificity of oligoclonal IgG in this disease is still unknown, as antibodies, for instance against measles virus or myelin basic protein, represent only a minimal part of intrathecally synthesized IgG.

Clone Cells↗

PubMedDate not supplied

[Anti-GFAP antibodies in the cerebrospinal fluid of patients with multiple sclerosis and other neurologic diseases].

The antibodies against glial fibrillary acidic protein (GFAP) in the CSF of patients with multiple sclerosis and other neurological diseases were determined. It was shown that the binding activity of CSF IgG with GFAP was higher in patients with MS and other organic neurological diseases as compared to control group (neurotics). The results support the idea, that in SM there is a general immune dysregulation, leading to production of a variety of autoantibodies against different antigens, including the presently shown GFAP.

Autoantibodies↗

PubMedDate not supplied

Catecholamines in the rat brain during hypoglycemic convulsions and coma.

The purpose of the study was to investigate the relation between the catecholamines: noradrenaline and dopamine in the rat brain on one hand and hypoglycemic convulsions and coma on the other. Concentrations of noradrenaline in the hypothalamus, brain stem and cerebral cortex were decreased during hypoglycemic convulsions and were lower during coma than those during convulsions. Dopamine concentration in the striatum was decreased during convulsions and coma. It was shown that the decrease in concentration of catecholamines was a result of hypoglycemia but not of insulin action itself. Clonidine- alpha 2 agonist accelerated occurrence and prolonged duration of hypoglycemic convulsions. Haloperidol-dopamine receptor blocker had no effect on the time of occurrence or duration of convulsions and coma. The results indicate that noradrenaline may exert an inhibitory influence on hypoglycemic convulsions. No evidence has been provided to support involvement of dopamine in the control of hypoglycemic convulsions and coma.

Animals↗

PubMedDate not supplied

[Limited value of colloidal tests in CSF in the diagnosis of multiple sclerosis].

In 136 clinically definite MS cases the comparison was performed of intrathecal IgG production and detection of oligoclonal bands, evaluated by immunological methods with results of colloidal tests. The obtained results show, that the left side precipitation in colloidal tests indicates the presence of intrathecal IgG production, what was a definite value in the diagnosis of MS. However, the value of colloidal tests is very much limited by several times lower sensitivity of colloidal tests in detection of intrathecal IgG production in comparisons with the evaluation of intrathecal production and detection of oligoclonal bands by the immunological methods.

Functional Laterality↗

PubMedDate not supplied

[The immunologic reaction in patients with ischemic stroke].

The humoral and cell mediated immunity was studied in 75 cases of completed ischaemic stroke. The results demonstrated only in few cases intrathecal IgG production and presence of oligoclonal IgG bands. A significant relative drop of cell count with CD3 reactivity and increase of cells with reactivity Anti-HLADR and CD11 was observed in peripheral blood. The results demonstrate that in the course of ischaemic stroke various immunological reactions may occur in particular cases. However, there exist no evidence that repeated stroke leads to immunization of the patient.

Aged↗

PubMedDate not supplied

[Determination of IgG subclass antibodies to the basic myelin protein in patients with multiple sclerosis].

The aim of the study was to determine IgG subclasses of MBP antibodies in patients with multiple sclerosis. The enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies against human IgG subclasses was applied. Anti-MBP antibodies were found in the CSF of 16% and in the sera of 20% patients with MS. MBP antibodies belonged to IgG1, IgG2 and IgG3 subclasses (in the CSF) and to IgG1 and IgG3 subclasses (in the sera). The role of MBP antibodies in context of their IgG subclasses distribution is discussed.

Antibodies, Monoclonal↗

PubMedDate not supplied

[Trials of monosialoganglioside (Sygen) treatment in ischemic stroke].

65 patients with ischaemic stroke were treated for 6 weeks by intramuscular injections of Sygen--monosialoganglioside 40 mg daily. The results were double-blinded and compared with those in placebo group (Vitamin PP). The results evaluated by Mathew scale did not show any significant differences between both groups. However in the Sygen group we have noticed more patients with very good therapeutic results (almost complete recovery of functions). Immunological studies have not revealed any reaction, which may be considered as a result of allergogenic action of monosialoganglioside.

Adult↗

PubMedDate not supplied

[The effect of azathioprine treatment on IgG subclasses in multiple sclerosis].

IgG and its subclasses: IgG1, IgG2, IgG3 and IgG4 were determined in the CSF and sera from 20 patients with multiple sclerosis before and after treatment with azathioprine. The effect of the treatment on IgG and IgG subclasses intrathecal synthesis was studied as well. There was a decrease in IgG level in the CSF of MS patients after the treatment with azathioprine. This was mainly due to the decrease of IgG1 and IgG2 subclasses levels. There was no evidence of the influence of azathioprine treatment on IgG and its subclasses levels in MS sera as well as on IgG and IgG subclasses intrathecal synthesis.

Adult↗

PubMedDate not supplied

[Effects of prednisone and azathioprine treatment on oligoclonal IGG in the cerebrospinal fluid of multiple sclerosis patients. Preliminary communication].

The influence of high-dose prednisone and azathioprine therapy on oligoclonal IgG in the CSF of MS patients was studied. The oligoclonal bands were present in 93% of patients treated with prednisone and in 90% of patients treated with azathioprine. After the treatment with prednisone, the oligoclonal bands density was diminished in 3 patients, and after the treatment with azathioprine in 1 patient. The number of bands and their pattern remained the same.

Anti-Inflammatory Agents↗

PubMedDate not supplied

[Subclasses of IgG in patients with neurosyphilis].

IgG subclasses levels in the CSF and sera were measured in 15 patients with neurosyphilis. Intrathecal IgG and IgG subclasses synthesis was studied as well. There was an elevation of IgG1 and IgG3 subclasses in the CSF and IgG1 elevation in the sera of patients with neurosyphilis. General IgG index was elevated in 46% of the patients. This was due mainly to IgG1 and IgG3 intrathecal synthesis. Oligoclonal IgG bands were present in the CSF in seven out of 15 studied patients. These results may be helpful in the first stage of the diagnostic procedure before specific serological tests are performed.

Adult↗