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Biomedical subjects

J Liu

Publications and source records attributed to J Liu.

At least 829 records · Page 46Linked to original sources
PubMed2000

[MMF and CyA in the prevention of early acute rejection after renal transplantation].

OBJECTIVE: To investigate the effect of MMF and low dose CyA on the prevention of early acute rejection after renal transplantation. METHODS: 146 patients with kidney transplantation were analyzed retrospectively from December 1997 to January 1999. These patients were divided into two groups according to the immunosuppressive regimen: Aza group (78 patients) and MMF group (68 patients). All patients met the following criteria: HLA mismatch </= 3 loci, lymphocytes toxicity test < 10%, PRA < 20% for second or third transplantation, warm ischemia time 5-11 minutes, and cold ischemia time 3-24 hours. RESULTS: There were 24 patients with acute rejection episodes (30.77%) in the Aza group; and 10 patients (14.71%) in the MMF group, (P < 0.05). The dosage of CyA and blood concentration of CyA were significantly different; 6.00 +/- 1.21 mg x kg(-1) x d(-1) and 286.00 +/- 20.02 microg/L in the Aza group; 4.00 +/- 1.14 mg x kg(-1) x d(-1) and 204.00 +/- 20.18 microg/L in the MMF group (P < 0.001). CONCLUSIONS: MMF with low dose CyA based triple regimen could prevent the acute rejection episodes effectively at the early period of post transplantation. This triple regimen can reduce the acute rejection episodes by 50%. To some extent, low dose CyA may avoid nephrotoxicity. The long-term effect of this regimen on allograft is still needed to observe.

Adolescent↗
PubMed2000

[The modulating effect of panax pseudoginseng wall saponins on the DAG-PKC signal pathway and TNF secretion of macrophages].

OBJECTIVE: To explore the roles of panax pseudoginseng wall saponins on the DAG-PKC signal pathway and TNF secretion of macrophages. METHODS: The changes of the activities of postburn inositol lipid signal system factors such as PLC, DAG, cytomembrane PKC, cytoplasma PKC, and intracellular calcium concentration and the secretory amount of macrophage TNF were observed. RESULTS: Panax pseudoginseng wall saponins could reduce the increased PLC activity from 57.58 +/- 8.19 to 27.00 +/- 2.31 and the intracellular calcium concentration from 393.18 +/- 392.62 to 90.56 +/- 7.21. With the role of panax pseudoginseng wall saponins, DAG activity reduced from 488.10 +/- 40.20 to 288.30 +/- 30.00, cytomembrane PKC activity decreased from 3081.50 +/- 698.50 to 1699.50 +/- 218.50, and cytoplasmic PKC activity from 2 188.60 +/- 258.30 to 848.40 +/- 138.30. The secretory TNF amount of macrophage decreased by 55%. CONCLUSION: Panax pseudoginseng wall saponins might play an very important role in the modulating of the DAG-PKC signal pathway and decreased TNF secretion of macrophage.

Animals↗
PubMed2000

[Effect on keratocyte-mediated collagen degradation by Pseudomonas aeruginosa].

OBJECTIVE: To study the pathogenesis of cornea melting (ulceration) by pseudomona (P) aeruginosa for instruction of clinical treatment. METHODS: Type I collagen gels with or without suspended keratocytes were incubated for 24 hours under medium containing sterile P. aeruginosa culture broth. Native collagen fibrils were removed from the media by ultrafiltration. The ultrafiltrates were then hydrolyzed, and the amount of hydroxyproline was measured spectrophotometrically. The effect of a synthetic matrix metalloproteinase (MMP) inhibitor, Galardin, on collagen degradation was also examined. RESULTS: P. aeruginosa broth induced type I collagen gel degradation directly. In the presence of keratocytes, degradation by P. aeruginosa broth was enhanced. Galardin significantly reduced the amount of collagen degraded by P. aeruginosa culture broth, no matter keratocytes were present or not. CONCLUSION: P. aeruginosa culture broth directly degrades type I collagen and also increases keratocyte-mediated collagen degradation. The result is helpful to the clinical treatment of cornea melting caused by P. aeruginosa, and the mechanism should be further studied.

Animals↗
PubMed2000

[Secondary glaucoma after penetrating keratoplasty].

OBJECTIVE: To investigate the etiology and management of secondary glaucoma after penetrating keratoplasty (PKP). METHODS: Eighty cases (80 eyes) with secondary glaucoma occurring in 1,450 cases (1,500 eyes) of PKP, its causes, time of onset and the relationship with the primary disease were analyzed. According to different causes, they were treated with different therapies. RESULTS: The intraocular pressure (IOP) of 63 cases (63 eyes) became normal after drug treatment for 2 - 3 weeks. In 17 cases (17 eyes) who had received antiglaucomatous operations, 13 cases' (13 eyes) IOPs were normal and 4 cases' (4 eyes) still high. CONCLUSIONS: Accurate perioperative management can decrease the incidence of secondary glaucoma after PKP. The majority of secondary glaucoma can be controlled by different therapies according to different causes.

Adolescent↗
PubMed2000

[A case control study on the distribution of apolipoprotein AI gene polymorphisms in the survivors of atherosclerosis cerebral infarction].

OBJECTIVE: To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5' - end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population. METHOD: Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron - 1 (+83 bp). RESULTS: (1) Both ACI and control groups M1 + + and M1 + - were major frequent genotypes in Beijng area, M2 + + were major frequent genotypes in intron - 1; (2) M1 + - genotype appeared more common among patients than in controls (0.487 vs 0.368, P < 0.05, OR = 1.64); (3) Individuals with M1 + -/M2 + + haplotype were significantly increased in patients compared with controls (0.437 vs 0.314, P < 0.05, OR = 1.70). CONCLUSION: It is suggested that both M1 + - genotype and M1 + -/M2 + + haplotype of ApoAI gene might associate with ACI in our study.

Apolipoprotein A-I↗
PubMed2000

[The recognition of the epidemic area of tsutsugamushi disease on Nan Peng Lie Island in China and the strategy of prevention].

OBJECTIVE: To investigate the region for the epidemic area of tsutsugamushi disease. METHODS: Epidemiological studies, Orientia tsutsugamushi isolation, and preventive measures were used. RESULTS: The region belonged to epidemic area of south subtropical zone. The main host was found Rattus norvegicu. The main biological vector was Leptotrombidium deliens. The seasonal trends of the quantity of Rattus norvegicu and Leptotrombidium deliens were consistent with the incidence change of the disease in the region. Orientia tsutsugamushi has been isolated from Rattus norvegicu and Leptotrombidium deliens. Data showed that Orientia tsutsugamushi isolated strains mainly belongs to Karp. Sero-epidemiological data showed that antibodies of Orientia tsutsugamushi are 100.0% positive in the residents of the region and 4.0% in army men. After taking comprehensive preventive measures, the incidence was descending. CONCLUSION: Nan Peng Lie island was proved an epidemic area of tsutsugamushi disease.

Animals↗
PubMed2000

[The relationship between polymorphism of angiotensinogen gene and essential hypertension].

OBJECTIVE: To study the renin-angiotensin system (RAS) involvement in the hormonal regulation of BP and blood volume and the relationship between angiotensinogen (AGT) was the sole preliminary acting materials in the RAS. METHOD: Population-based case-control study was conducted using PCR-RFLP techniques to explore the relationship between the M235T polymorphism of AGT and EH in Wangxin township, Jiading District of Shanghai. RESULTS: The frequency of TT genotype in EH cases was significantly higher than in controls (42.8% and 33.0%). The odds ratio (OR) for those exposed to TT genotype was 3.61, comparing with MM genotype. It was suggested that TT genotype be the susceptible genotype of EH in Wangxin. The frequency of allele T in EH cases was 73.89%, significantly higher than that of 60.31% in controls. With the increase of allele T, the risk of EH also increased (P < 0.05). In EH cases, the BMI increased in the order of MM, MT and TT, and significant differences between the groups were observed. CONCLUSION: TT genotype is the susceptible genotype of EH in Wangxin.

Aged↗
PubMed2000

[Effects of chronic exposure to lead on long-term potentiation in hippocampal CA1 area of young rats in vivo].

OBJECTIVE: To study the effects of chronic exposure to lead on learning and memory function in young rats, based on the plasticity of their synapse function. METHODS: With method of extracellular glass microelectrode recording, effects of exposure to lead at different concentrations on long term potentiation (LTP) induction in hippocampal CA(1) area of young rats in vivo were observed. RESULTS: Incidence of LTP and mean amplitude increase after application of high frequency stimulus decreased, as blood lead in rats reached (3.28 +/- 0.88) micromol/L, even causing long term depression. To the exposed rats, their mean increase ratio in their population spike amplitudes after high-frequency stimulation correlated inversely with their brain lead load. CONCLUSION: Chronic lead exposure could damage in vivo LTP induction and maintenance in the CA(1) area in hippocampus of rats, and the severity of damage increased with the extent of exposure to lead.

Animals↗
PubMed2000

[The effect of fluoride-arsenic exposure on the lipid peroxidation and antioxidation of the offspring of rats].

OBJECTIVE: To provide information on the effects of the offspring of rats exposed to fluoride-arsenic. METHODS: The levels of lipid peroxidation and the abilities of antioxidation were determined in the blood of the rats and their offsprings under two generations-one nest reproductive test. RESULTS: The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the blood decreased with the increase of exposure dose. For example, the activity of SOD, was 14.56, 13.74, 11.89 and 11.21 micromol x min(-1) x mg Hb(-1) in different dose groups of F(2), respectively. In contrast, the concentration of lipid peroxides (LPO) increased. Eight weeks after exposure, the activities of SOD and GSH-Px increased, the activity of SOD was 13.97, 13.55, 13.47 and 12.76 micromol x min(-1) x mg Hb(-1), respectively, and the concentration of LPO returned to normal level. CONCLUSION: Fluoride-arsenic exposure can cause oxidative damage of the rat's offspring.

Animals↗
PubMed2000

[A histopathologic and immunohistochemical study of prognostic factors in esophageal squamous cell carcinoma].

OBJECTIVE: To investigate the prognostic value of various histopathological features and expression of immuno-phenotypes in esophageal squamous cell carcinoma (ESCC). METHODS: The tumor samples from 97 primary ESCC patients with potentially curative resection were evaluated for the following parameters: tumor size, grade of differentiation, pattern of invasion, depth of invasion, degree of lymphocyte infiltration, lymph node metastasis, TNM stage and p53, EGFR, c-erbB-2, nm23, cathepsin D protein expression. The prognostic impact of these parameters were analyzed by univariate and multivariate survival analyses. RESULTS: In the univariate Kaplan Meier analysis, poor differentiation, infiltrating growth pattern, invasion reaching adventitia, absent lymphocytic infiltration, lymph node metastasis, high staging, EGFR positive expression and nm23 negative expression were all associated with lower survival rate (Log-rank test, P < 0.05, respectively). However, the multivariate Cox analysis demonstrated that only pattern of invasion (RR = 2.000), degree of lymphocyte infiltration (RR = 0.509), TNM stage (RR = 1.733), EGFR expression (RR = 1.812) and c-erbB-2 expression (RR = 2.364) were significant prognostic factors (P < 0.05, respectively). CONCLUSION: For ESCC, the pattern of invasion, degree of lymphocyte infiltration, EGFR and c-erbB-2 protein expression had independent prognostic values as well as the TNM stage. Comprehensive analysis of these parameters can be helpful to identify biological behavior of ESCC and predict more accurately the prognosis of patients with ESCC.

Adult↗
PubMed2000

[Effect of mouse p53 minigene on lung cancer cells with different 172 structures regulated by tetracycline].

OBJECTIVE: To investigate the effect of mouse 172 wild-type p53 (Arg), pseudo-wild-mutant-type p53 (Arg-->Leu) and mutant-type p53 (Arg-->His) induced by absence of tetracycline on the growth of PG cell line. METHODS: Three variant types of p53 minigene were sub-cloned by gene recombination into an expression vector which was controlled by tetracycline. Through LipofectAMINE, the vectors were transfected into p53 defective PG (248CGG-->CTT) cells, and the transfectants were screened in the selecting medium containing puromycin. Tumor suppressing effects were studied by MTT absorption, flow cytometry and Western blotting. RESULTS: Wild-type p53 and pseudo-wild-mutant-type p53 could lead cells to decrease their growth rates, arrest cell cycle and transactivation of p21(WAF1). Mutant-type p53 was defective in tumor suppression. CONCLUSION: Wild-type p53 and pseudo-wild-type p53 may inhibit cell growth and induce cell cycle arrest. Some p53 variants such as 172 Arg-->Leu can still retain the tumor suppression function of the wild-type.

Amino Acid Substitution↗
PubMed2000

[Establishment and biological characteristics of a novel erythroleukemia cell line (HIE1)].

OBJECTIVE: To establish a novel leukemia cell line and characterize its biological characteristics. METHOD: The cell line was established by liquid cell culture. The genetic marker was analyzed by R-banding and reverse transcriptase-polymerase chain reaction (RT-PCR), cell morphology by microscopy, electron microscopy and histochemical staining, cell surface antigen by monoclonal antibody, hemoglobin by hyperomethemoglobin measurement and electrophoresis, erythroid differentiation by benzidine-staining, and monocyte-macrophage differentiation by cell morphology and phagocytosis. RESULTS: A novel erythroleukemia cell line (HIE1), with original cell genetic marker (Ph chromosome, bcr/abl fusion gene rearrangement), was established from a CML patient in blast crisis, and has been passaged for over 60 generations. Myelomonocyte marker and hemoglycoprotein A were found on the cell surface. HIE1 cells contained hemoglobin, the same HbA and HbA(2) bands as in normal individuals were displayed by Hb electrophoresis. The benzidine positive HIE1 cells were induced after exposure to 3.6 x 10(-4) mmol/L Ara-C. When HIE1 cells were treated with 100 ng/ml PMA for 3 days, one third of the cells became spindle in shape, and 6.5% of the cells exert phagocytosis. The cells were classified into two types with Wright-staining: one showing light blue cytoplasm and a few of cells with basophilic granules, the other showing dark blue cytoplasm with vacuoles and pseudopods without granules. In addition, POX, SB, CE stains were negative, and AE, PAS, ACP stains positive. Colony formation of the cells was 37%, the cell doubling time was 22 - 24 hrs, and EB virus detection was positive. CONCLUSION: A novel erythroleukemia cell line with bcr/abl fusion gene and characteristics of myelomonocytic and erythroid cells was established.

Cell Differentiation↗
PubMed2000

[Comparative study of three common G6PD gene mutations in Yao and Han People in Guangxi].

OBJECTIVE: To study G6PD gene mutation diversity in Yao and Han people in Guangxi and compare the genotypic frequencies. METHODS: The three common G6PD gene mutations in Chinese were investigated in 34 G6PD deficient individuals from Yao and 37 from Han people by using an amplification refractory mutation system (ARMS). RESULTS: Among 34 cases of G6PD deficiency of Yao people, the genotypic frequencies of G1376T, G1388A and A95G were 41.2%, 26.5% and 14.7%, respectively. A case of C1311T was detected. As compared with that in Han people, the respective frequencies were 16.2%, 40.6% and 5.4%. CONCLUSION: The main G6PD mutations in Yao people were the same with Han people in Guangxi, but G1376T mutation was higher than that in Han people. The C1311T mutation in Yao people in Guangxi was reported for the first time.

Adolescent↗
PubMed2000

[Modulation of prolactin (PRL) secretion in human uterine decidual cells at early pregnancy].

Decidualization is essential for implantation of embryo and maintenance of pregnancy in human. The mechanism of decidualization was investigated in this study by regulation of prolactin (PRL) release in cultured human decidual cells during first trimester of gestation. Progesterone significantly stimulated PRL secretion, but the effect of estrogen depended on its concentration, which was ineffective at the physiological level (10( 9) mol/L) but suppressed the stimulatory effect of progesterone at higher levels. Thus adequate proportion of estrogen and progesterone is important for the decidualization. Furthermore, RU486 dramatically inhibited PRL release, suggesting that the effect of progesterone was mediated, at least in part, through its receptor. cAMP at concentration higher than 10( 5) mol/L significantly increased PRL secretion, suggesting that the cAMP signal pathway might be involved in decidualization.

Cells, Cultured↗
PubMed2000

[Cell cycle analysis and pathological changes of malignant tumors treated with electrochemical therapy].

To investigate the mechanism and to observe the effectiveness of electrochemical therapy(ECT), 33 patients of late stage cancers which treated by ECT were included in this study. Flow cytometry (FCM) and pathology were used to observe the changes of tumor cells before and after ECT. Tumor cells of G1, G2/M, S phases and aneuploid cells were found to be killed almostly and the ratio of apoptosis was elevated greatly after ECT. Also, pathological evidence proved the death of tumor cells and some characters of apoptosis. So, ECT can non-selectively kill tumor cells and induce the apoptosis of them, and FCM was a way to evaluate the effectiveness of ECT.

Adult↗
PubMed2000

[The relationship of cellular DNA content with clinical stage and biological features of colorectal cancer].

The purpose of this study was to explore the relationship of cellular DNA content with clinical stage and biological features of cancer. Flow cytometry was performed on fresh specimens from 86 patients from 1997 to 1998. Forty-five (53.3%) specimens were found to contain cells with abnormal DNA (DNA nondiploidy). Although none of the sex, age, site, differentiation variables correlated with flow cytometric DNA ploidy, nondiploidy was associated with Dukes' stage and lymph node metastasis. Duke's A stage tumors were more frequently diploid than were more advanced tumors, but no difference between Duke's B, C and D stages were observed. These findings suggest that ploidy is associated with some pathological factors.

Aged↗