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Biomedical subjects

J Liu

Publications and source records attributed to J Liu.

At least 505 records · Page 28Linked to original sources
PubMed2001

[The dynamic change in the expression of interleukin 18 mRNA in the tissue of scalded rats after delayed resuscitation].

OBJECTIVE: To explore the dynamic change in the expression of interleukin 18 (IL-18) mRNA in the tissue of scalded rats after delayed resuscitation. METHODS: Wistar rats inflicted by 30% TBSA III degree scalding were employed as the model. Fifty-four rats were randomly divided into normal control (C), scalding and delayed resuscitation group (SD) and preventive group of selective decontamination of digestive tract (SDD) group. The RT-PCR was applied to determine the tissue contents of IL-18 mRNA in intestine, lungs, liver and kidneys at 2, 8, 16 and 24 PBHs. RESULTS: Plasma level of LPS increased significantly and reached summit level at 8 and 24 PBHs after delayed resuscitation after scalding (P < 0.01), but decreased obviously (P < 0.05) after the preventive application of SDD. Furthermore, the expression of tissue IL-18 mRNA in lungs, liver and kidneys since 2 PBHs increased evidently when compared to that before scalding and reached peak value at 8 PBH (P < 0.01) and lasted up to 24 PBHs. The expression of IL-18 mRNA could be inhibited to varying degrees by preventive application of SDD (P < 0.05-0.01). It was indicated by correlative analysis that plasma level of LPS was positively correlated to the expression of tissue IL-18 mRNA in intestine, lungs and liver (r = 0.298, 0.290 and 0.365 respectively with P < 0.05-0.01). CONCLUSION: The expression of tissue IL-18 mRNA in intestine, lungs, liver and kidneys increased significantly during early postburn stage and progressively enhanced thereafter. And the expression of tissue IL-18 mRNA in a variety of body tissue was significantly affected by post-traumatic endotoxemia.

Animals↗
PubMed2001

Low incidence of severe aGVHD and accelerating hemopoietic reconstitution in allo-BMT using lenograstim stimulated BM cells.

OBJECTIVES: To investigate the efficacy of accelerating hemopoietic reconstraction and reducing a graft versus host disease (GVHD) in Allo-BMT receiving lenograstim stimulated donor marrow and to assess the preliminary biological mechanism. METHODS: The donors for thirty patients (study group) with leukemia were given lenograstim 3-4 micrograms.kg-1.d-1 for seven days prior to marrow harvest. The results of subsequent engraftment in the recipients was compared with fifteen donors without G-CSF (control group). Five donors themselves were studied to assess the effects of lenograstion on hematopoietic progenitor cells and lymphocyte subsets in BM. RESULTS: The stimulated bone marrow contained a higher number of nucleated cells, CFU-GM and CD34+ cells (P < 0.01). The hematopoetic reconstitution was accelerated. Until granulocyte counts exceeded 0.5 x 10(9)/L and plalete counts exceeded 20 x 10(9)/L, the days were 16.7 +/- 3.2 and 18.4 +/- 3.0 days as compared with those of the control group (22.5 +/- 5.1 and 26.3 +/- 5.9 days respectively, P < 0.01). The incidence of grade II-IV aGVHD was very low, only one case with grade II aGVHD on the skin in the study group. Four out of fifteen patients (26.7%) in the control group had grade II-IV aGVHD (P < 0.05). The number of T lymphocyte subsets in the harvested BM stimulated by G-CSF changed. In comparison with the control group, CD4+ decreased and CD8+ increased significantly (P < 0.01). The changes of progenitor cells and T lymphocyte subsets in BM from pre- to post-G-CSF stimulation indicated that the percentage of CD4+ cells reduced (P < 0.05), that of CD8+ cells, and that of CD34+ increased (P < 0.01). The incidence of chronic GVHD and relapse of leukemia were not different significantly between both groups. CONCLUSIONS: Allogenic bone marrow transplant (Allo-BMT) donors given G-CSF can accelerate engraftment and minimize the incidence of severe aGVHD. There is a trend in favour of improved transplant-related complications.

Acute Disease↗
PubMed2001

Monitoring of antegrade selective cerebral perfusion for aortic arch surgery with transcranial Doppler ultrasonography and near-infrared spectroscopy.

OBJECTIVE: To evaluate the safety and efficacy of antegrade selective cerebral perfusion (ASCP) during aortic arch surgery as a means of extending the safe period of systemic circulatory arrest using multimodality neuromonitoring to objectively quantify the physiologic responses. METHODS: In twenty-two patients (all less than age 60) scheduled for repair of an aortic arch aneurysm, preoperative verification of effective collateral perfusion through both the carotid and vertebrobasilar arterial systems was documented with transcranial Doppler ultrasonography (TCD). During cardiopulmonary bypass, the sole arterial inflow from the pump was via the right subclavian artery. The magnitude of ASCP was quantified by TCD using peak middle cerebral artery velocity, while flow adequacy was measured by continuous regional cerebrovenous oxygen saturation (rSO2) using dual-wavelength spatially resolved near-infrared spectroscopy. RESULTS: All patients experienced an uneventful recovery. Flow in the middle cerebral artery became undetectable at ASCP < 5 ml.kg-1.min-1, so adjustments from a 15-20 ml.kg-1.min-1 baseline were used to maintain rSO2 above 50%. Furthermore, ASCP flow was highly correlated (P < 0.01) with both peak middle cerebral artery velocity and rSO2(r = 0.86 and 0.96, respectively). CONCLUSION: Neuromonitoring guided ASCP may be expected to extend the safe period and is at least partly responsible for the absence of neurologic complications in this patient cohort.

Adolescent↗
PubMed2001

Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene.

OBJECTIVE: To investigate BCL-6 gene mutations in Chinese populations with B-cell non-Hodgkin's lymphoma. METHODS: Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma (DLCL) and follicular lymphoma (FL). RESULTS: Nine cases were found to have base substitutions. The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% and (0.56-1.10) x 10(-2)/bp, respectively. CONCLUSIONS: The 5' regulatory region of the BCL-6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL.

5' Flanking Region↗
PubMed2001

Ultrastructure of intrahepatic biliary canaliculi and prognosis of congenital biliary atresia.

OBJECTIVE: To investigate the relationship between the ultrastructure of intrahepatic biliary canaliculi and the prognosis of congenital biliary atresia. METHODS: Liver biopsies from 25 patients who were operated on for congenital biliary atresia were examined under the transmission electron microscope. The relationship between the number of well-developed bile canaliculi and patient prognosis was studied. RESULTS: The survival rate for 13 patients with well-developed intrahepatic biliary canaliculi and 12 patients with poorly-developed intrahepatic biliary canaliculi were 92.3% (12/13) and 33.3% (4/12) respectively. The difference between two groups was statistically significant (P < 0.05). CONCLUSIONS: Intrahepatic biliary canaliculi ultrastructure could be used as one of the prognostic factors in congenital biliary atresia.

Bile Canaliculi↗
PubMed2001

Activity identification of anti-caspase-3 mRNA hammerhead ribozyme in both cell-free condition and BRL-3A cells.

OBJECTIVE: To study the transcription effects and cleavage activities of rat caspase-3 specific hammerhead ribozyme (Rz107) in both cell-free conditions and BRL-3A cells. METHODS: Rat caspase-3 gene fragment was cloned into the pGEM-T EASY vector under the T7 promoter control. The 32P-labeled caspase-3 transcript was the target-RNA. Rz107 genes designed against caspase-3 mRNA were cloned into vector p1.5 between 5'-cis-Rz and 3'-cis-Rz. 32P-labeled ribozyme transcripts were incubated with target-RNAs at different conditions and autoradiographed after denaturing gel-electrophoresis. Rz107 was electroporated into BRL-3A cells and the Rz107 expression was analyzed by RT-PCR. RESULTS: In cell-free conditions, Rz107 was active at 37 degrees C. The optimal temperature was 50 degrees C. The Km and Kcat were 14.13 nmol/L and 2.31.min-1 respectively. Intracellular cleavage efficiency of Rz107 was 37%, as analyzed by RT-PCR. This indicated that the design of Rz107 was correct, and Rz107 had the activity of common enzymes. CONCLUSIONS: Rz107 in cell-free conditions possessed perfect specific catalytic cleavage activity, and it can also cleave the target RNA successfully in cells. The results illustrate the feasibility of ribozyme therapy as a potential alternative approach for treating liver disease caused by apoptosis.

Animals↗
PubMed2001

Batroxobin against anoxic damage of rat hippocampal neurons in culture: morphological changes and Hsp70 expression.

Batroxobin, the thrombin-like enzyme, is used for therapeutic defibrination. We have found that batroxobin has good therapeutic effect in ischemic reperfusion rats and clinical practices in vivo. But we have not studied the neuroprotective effect of batroxobin on anoxic hippocampal neurons in vitro. The purpose of this study was to obtain further information on the mechanism of the batroxobin-induced neuroprotection and examine the neuroprotective effect on neurons exposed to anoxia. The effect of batroxobin on anoxic damages in cultured hippocampal neurons of neonatal rats was investigated by using morphological changes and heat shock protein 70 Kd (Hsp70) immunoreactive expression as indicators. The results indicate that batroxobin, besides its defibrination, may have a direct neuroprotective effect on anoxic damage of hippocampal neurons.

Animals↗
PubMed2001

Selecting informative genes with parallel genetic algorithms in tissue classification.

Recent advances in biotechnology offer the ability to measure the levels of expression of thousands of genes in parallel. Analysis of such data can provide understanding and insight into gene function and regulatory mechanisms. Several machine learning approaches have been used to aid to understand the functions of genes. However, these tasks are made more difficult due to the noisy nature of array data and the overwhelming number of gene features. In this paper, we use the parallel genetic algorithm to filter out the informative genes relative to classification. By combing with the classification method proposed by Golub et al. and Slonim et al., we classify the data sets with tissues of different classes, and the preliminary results are presented in this paper.

Algorithms↗
PubMed2001

Optimal management of postpartum hemorrhage.

OBJECTIVE: To review the management of postpartum hemorrhage (PPH). METHODS: Fifteen cases of severe PPH were retrospectively analyzed for clinical parameters and management. RESULTS: Nine patients had a hysterectomy while selective arterial embolization was carried out in 7 cases. One patient had focus clearance. The main causes of postpartum hemorrhage requiring operative management were disseminated intravascular coagulation (DIC), uterine arteriovenous fistula and malformation, placental abnormality, uterine myoma and laceration. CONCLUSION: Upon recognition of postpartum hemorrhage, the most effective management should be initiated. Every effort should be made to stabilize the patient and maintain her reproductive capability.

Adult↗
PubMed2001

[Expression of Fas on monocytes and the level of plasma soluble FasL in patients with chronic renal failure].

OBJECTIVE: The dysfunction of monocytes in uremic patients has been demonstrated to be associated with monocyte accelerated apoptosis. The study was conducted toelucidate the mechanisms by which monocyte apoptosis was induced. METHODS: Seven non-dialyzed chronic uremic patients and 18 hemodialysis (HD) patients were enrolled in the study. Fifteen healthy volunteers were selected as controls. The expression of Fas and Fas ligand (FasL) was examined by immunofluorescent staining and flow cytometer analysis. The levels of soluble FasL (sFasL) in plasma were analyzed by enzyme-linked immunosorbent assay (ELISA). The percentage of apoptotic cells in monocytes cultured in vitro with recombinant human FasL (rHu-FasL) was quantitated by DNA content analysis after extraction of the degraded DNA. Monocyte survival rate was analyzed by 5-diphenyl tetrazolium bromide (MTT) staining. RESULTS: Expression of Fas on monocytes was significantly higher in uremic and HD patients than that in healthy subjects. Higher levels of Fas expression were demonstrated on monocytes from HD as patients compared with those from non-dialyzed patients. There was no difference in monocyte Fas expression between pre- and post-dialysis session and between patients using polysulfone and cellulose dialyzer. There was no detectable FasL expression on monocytes from both uremic patients and healthy controls. Plasma sFasL could be detected in uremic and HD patients, but not in healthy controls. There was no significant difference in levels of plasma sFasL between non-dialyzed and HD patients, pre- and post-dialysis session, and patients treated with polysulfone and cellulose dialyzer. When monocytes were cultured in vitro with rHu-FasL for 2 hours, higher levels of apoptosis and lower survival rate were found in monocytes from HD patients as compared with those from healthy controls. CONCLUSION: The up-regulated expression of functional Fas on monocytes and the presence of sFasL in plasma may contribute to the accelerated apoptosis of monocytes seen in uremic patients.

Adult↗
PubMed2001

[Alveolar macrophage and airway inflammation in patients with chronic obstructive pulmonary disease].

OBJECTIVE: To investigate the effects of alveolar macrophage(AM) on airway inflammation of chronic obstructive pulmonary disease(COPD). METHODS: Broncho alveolar fluid(BALF) and bronchial mucosa biopsies were performed as usual. The levels of macrophage inflammatory-1 alpha (MIP-1 alpha), gelatinase B (MMP-9), interleukin(IL)-8 in BALF and the culture supernatants of AMs were measured with ELISA. The levels of endothelin (ET) were measured with radioimmunoassay method. The AMs expressing lymphocytic functional associated antigen-1 (LFA-1) in BALF, the AMs in mucosa and the thickness of mucosa were measured with immunocytochemistry and immunohistochemistry technique. RESULTS: The levels of MIP-1 alpha, MMP-9, IL-8 and ET in the culture supernatants of AMs in patients with COPD were (1 446.9 +/- 126.7) microg/L, (16.98 +/- 5.15) microg /L, (43.98 +/- 5.54) ng/L, (168.10 +/- 21.22) microg/L respectively; while those were (1 123.5 +/- 90.6) microg/L, (5.24 +/- 0.93) microg/L, (25.99 +/- 6.05) ng/L, (124.68 +/- 9.96) microg/L in a control group. The number of macrophages in mucosa in patients with COPD was (1.71 +/- 0.48) cells/Hp, while that was (0.59 +/- 0.18) cells/Hp in the control group (0.63 +/- 0.25) x 10(4). Statistical significance was found between the two groups. The number of AMs and polymorphonuclear leucocyte in BALF were inversely correlated with forced expiratory volume in one second as percentage of predicted value(FEV(1)% Pre) (r = -0.511, P < 0.01; r = -0.562, P < 0.01). The levels of MMP-9 and ET in the culture supernatants of AMs were inversely correlated with FEV(1)% Pre(r = -0.678, P < 0.01; r = -0.871, P < 0.01). Both the level of MIP-1 alpha and the positive number of AM expressing LFA-1 in BALF were positively correlated with the number of AMs in BALF(r = 0.572, P < 0.01; r = 0.625, P < 0.01). CONCLUSION: AM may be involved in the process of airway inflammation and subsequent airway obstruction.

Adult↗
PubMed2001

[Airway alveolar macrophages and LFA-1 expression in chronic bronchitis].

OBJECTIVE: To assess the relationship between the expressions of lymphocyte function associated antigen-1 (LFA-1) in macrophages in both BALF and bronchial mucosa and the severity of airway inflammation in patients of chronic bronchitis. METHODS: Bronchial alveolar lavage and bronchial mucosa biopsy were performed in 18 patients with stable stage of chronic bronchitis (CB). LFA-1 positive alveolar macrophage (AM) was measured by immunocytochemistry stain. The percentages and number of AM in BALF and bronchial mucosa were also calculated and the thickness of mucosa measured. RESULTS: (1) The numbers of AM and neutrophils of BALF in CB were significantly higher than those in healthy group (P < 0.01, respectively). So did the percentage of neutrophils of BALF (all P < 0.05). (2) The number [(4.36 +/- 2.11) x 10(4)/ml] and percentage [(24.6 +/- 5.8)%] of positive AM expressing LFA-1 in CB were significantly higher than those [(0.63 +/- 0.25) x 10(4)/ml, (10.6 +/- 2.7)%] in healthy group (all P < 0.01, respectively). The number of AM in BALF was positively correlated with that of positive AM expressing LFA-1 (r = 0.624 7, P < 0.01). The number of CD(68)(+) cells [(21.6 +/- 4.6)/HP] in mucosa and the thickness of mucosa [(0.20 +/- 0.09) micrometer] in CB were significantly greater than those [(10.0 +/- 3.4)/HP, (0.08 +/- 0.04) micrometer] in healthy group (P < 0.01, respectively). They were inversely corrlated with the value of FEV(1)%pre (r = -0.5875, P < 0.01; r = -0.655, P < 0.05). CONCLUSIONS: Increased expression of LFA-1 may be one of important causes leading to the accumulation and migration of AM in the airway of patient with CB. AM infiltration and the thickening of bronchial mucosa are closely related to the decline of FEV(1).

Adult↗
PubMed2001

[Ecological adaptability of Suffolk sheep and Polled Dosert sheep introduced from Australia to Xinjiang and their hybrid utilization].

The physiological characters, reproductive rate and comprehensive adaptability of Suffolk sheep and Polled Dosert sheep introduced from Australia to Xinjiang Region were measured. The results showed that the Suffolk sheep and the Polled Dosert sheep were similar or higher than those in Australia in productive rate, and body weight. The comprehensive adaptability of Suffolk sheep and Polled Dosert sheep were 98.80% and 98.74%, respectively. The performance of fattening, slaughtering of F1 of Suffolk and Polled Dosert sheep with Kasak sheep were better than that of Kazak sheep.

Adaptation, Biological↗
PubMed2001

Clinical characteristics of neonatal lupus erythematosus.

Neonatal lupus erythematosus is a rare disorder caused by transplacental autoantibodies from the mother to the fetus. This syndrome is characterized by congenital heart block and/or cutaneous lesion. A total of 10 cases of neonatal lupus erythematosus were diagnosed at the National Taiwan University Hospital from 1988 through 1998. The incidence of cardiac anomaly and other clinical features in patients with neonatal lupus erythemayosus in this study was compared with those in previous studies. Results showed that 50% of patients had congenital heart block and/or cutaneous lesion, which is compatible to previous statistics. However, the findings showed that 80% of the patients were female and 90% of the mothers had acquired an autoimmune disorder, which were much higher compared with that of other studies. To date, no definite treatment is suggested prenatally to prevent the occurrence of congenital heart block, but careful maternal screening and serial fetal echocardiogram are warranted.

Antibodies, Antinuclear↗
PubMed2001

[Intrauterine growth retardation and cerebral palsy].

OBJECTIVE: To obtain the prevalence of cerebral palsy in children affected with intrauterine growth retardation (IUGR) and to explore the association between IUGR and cerebral palsy. METHODS: A cross-sectional survey was carried out among children aged under 7 years in seven cities of south Jiangsu Province. RESULTS: There were 5.6% (95% CI 5.6% to 5.7%) of the children classified as IUGR with a bimodal distribution, the highest in the 35-36 and 42-43 weeks of gestational age. The prevalence of cerebral palsy (CP) in IUGR children was 5.7@1000 (95% CI of 4.7@1000 to 6.9@1000), 4.2 times more than that in non-IUGR children (95% CI of 3.4-5.2). The prevalence of CP in IUGR children of preterm and term delivery (< 37 weeks and > or = 37 weeks of gestational age) was 1.9 (95% CI of 1.2 to 3.0) and 4.2 (95% CI of 3.3 to 5.3) times more than that in non-IUGR children, respectively. Multiple logistic regression analysis showed the effect of IUGR on CP for children with birth weight greater than 2,500 g, after controlling for birth weight and gestational age. CP prevalence in IUGR children was two times more than that in non-IUGR children. Furthermore, prevalence of IUGR in children with birth weight lower than the 3rd percentile and in those with the 3rd percentile to lower than the 10th percentile was 3.4 and 1.8 times more than that in non-IUGR children, respectively. CONCLUSIONS: The prevalence of cerebral palsy in IUGR children was 5.7@1000, 4.2 times that in non-IUGR children and IUGR is an independent risk factor for cerebral palsy.

Birth Weight↗
PubMed2001

[Important bio-thermal physical problems and latest advancement in laser cell engineering].

The recently emerging technique of laser microsurgery (optical tweezers, optical scissors, etc.) is providing a new precise, sterile method for the cell engineering practices such as introduction of external gene into an object cell, cell-fusion, and trapping or transportation of microscopic objects (cells or chromosomes etc.). The thermal effects thus induced usually proved to be critical factors for successful operation of this method. In order to meet the requirement for the rapid development in this territory, some important bio-thermal physical problems and the corresponding research subjects in this area were comprehensively summarized. Difficulties and critical issues were discussed. The latest advancement of the laser cell engineering was also described. This review is attempted to bridge up the gap between bioengineering and thermal science fields and then to enhance the rapid progress of laser microsurgery.

Biotechnology↗
PubMed2001

[The clonal origin of the myeloma cells secreting three kinds of immunoglobulin from a multiple myeloma patient].

OBJECTIVE: To explore the clonal origin of myeloma cells from a multiple myeloma (MM) patient with 3 kinds of immunoglobulin (Ig). METHODS: Six primers for IgH variable region gene families VH1-VH6 and one primer of common join region (J) were used to perform reverse transcriptase polymerase chain reaction(RT-PCR). Five oligonucleotides of constant region gene (C mu, C delta, C gamma, C alpha and C epsilon) were also used as reverse primers. Different combination of primers were used to perform PCR. PCR products of IgH gene rearrangement were cloned and sequenced. RESULTS: A sharp and dense gene band appeared by using Ig VH3 gene family primer and J primer. The specific IgH gene bands also appeared by using primers of VH3 with C mu and C gamma and C alpha but not by primers of VH1, VH2, VH4, VH5 with J as well as VH3 with C delta and C epsilon. It indicated that there were IgM, IgG and IgA gene rearrangement. These gene products were cloned and sequenced. All 3 IgM, IgG and IgA gene sequences are identical. CONCLUSION: Despite the oligoclonality of MM cell, the sequencing results of 3 IgH genes showed that all the malignant cells were monoclonal origin.

Base Sequence↗