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Biomedical subjects

J Lisiewicz

Publications and source records attributed to J Lisiewicz.

At least 55 records · Page 3Linked to original sources
PubMed1985

N-acetyl-beta-D-glucosaminidase in lymphocytes of patients with diabetes mellitus.

In 55 patients with diabetes mellitus the significant quantitative increase in numbers of N-acetyl-beta-D-glucosaminidase-positive lymphocytes in the peripheral blood has been examined by cytochemical methods. The enzyme-positive lymphocytes of these patients were characterized by prevalence of extralysosomal localization of the enzyme and a decrease in the numbers of those cells having intact enzyme-positive lysosomes. No association between these alterations and therapy with insulin or oral hypoglycemic drug and overweight could be stated.

Acetylglucosaminidase↗
PubMed1985

Leucine aminopeptidase of neutrophils and lymphocytes in patients with cancer.

The leucine aminopeptidase activity has been determined by using the cytochemical method of Burston and Folk in peripheral blood neutrophils and lymphocytes of 45 patients with various malignancies. Lung cancer, carcinoma of the stomach and cancer of the colon was diagnosed in 24, 16, and 5 patients, respectively. Patients with metastases showed a significantly higher activity of the enzyme if compared with that in the control group of healthy subjects and patients without metastases. The percentage of enzyme-positive lymphocytes was elevated significantly in patients with metastases whereas a total percentage of lymphocytes with regard to differential leukocyte count was diminished both in patients with and without metastases. The absolute count of neutrophils was elevated both in patients with and without metastases. The authors discuss the significance of their observation with regard to the antitumor cytotoxic effect of neutrophils and lymphocytes.

Colonic Neoplasms↗
PubMed1985

Activity of some lysosomal enzymes in peripheral blood lymphocytes of patients with lung cancer. A cytochemical study.

In 33 patients with lung cancer (6 women and 27 men, aged at average 61.2 years) the activity and intracellular localization of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase in peripheral blood lymphocytes were determined by means of semiquantitative cytochemical methods. In comparison to the control group of healthy subjects, the patients with lung cancer showed increased counts of acid phosphatase-positive lymphocytes with granular-diffuse cytochemical reaction, increased counts of beta-glucuronidase-positive lymphocytes with solely granular type of reaction and increased numbers of N-acetyl-beta-glucosaminidase-positive cells showing the granular, granular-diffuse and diffuse type of reaction. The total count of beta-glucuronidase-positive and N-acetyl-beta-glucosaminidase-positive lymphocytes was significantly elevated in these patients. The authors discuss the significance of their observations for evaluating lymphocyte response in patients with lung cancer.

Acetylglucosaminidase↗
PubMed1985

[The hematopoietic system in laboratory animals exposed to benzene].

The authors analysed, basing on literature, the mechanism of multitoxic effects of benzene and lesions in the peripheral blood of affected animals. The effects of chronic benzene poisoning upon erythrocytes and erythropoiesis, granulocytes and granulopoiesis, lymphocytes and lymphopoiesis, thrombocytes and thrombopoiesis were presented. Differences were pointed out in toxic effects of benzene varying with the kind, concentration and administration route of benzene and quantitative and qualitative differences in the fodder given to animals during the experiment.

Animals↗
PubMed1985

Aging and lymphocyte lysosomes in man.

It has been stated in 120 healthy subjects of varying age that last three decades of human life that is between 60 and 90 year of life, the dramatic decrease in numbers of peripheral blood lymphocytes having intact lysosomes containing acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase occurs. In contrast, the first five decades of life are characterized by relative stability of enzyme-positive lysosomal apparatus of lymphocytes. Above studies have been performed using semiquantitative cytochemical methods. The authors discuss their results with regard to similar phenomenon of lysosomes disintegration in lymphocytes in patients with malignancies.

Acetylglucosaminidase↗
PubMed1984

Deficiency of N-acetyl-beta-D-glucosaminidase in neutrophils of cancer patients.

It has been suggested that intracellular enzymatic deficiencies of neutrophils may represent another cancer risk factor. This concept is based on data indicating the cytotoxic effects of neutrophils on tumor cells. In 196 patients with various malignancies, precancerous states and states predisposing to malignancies, N-acetyl-beta-D-glucosaminidase activity has been determined in peripheral blood neutrophils using a semiquantitative cytochemical method. A statistically significant deficiency of this enzyme in neutrophils has been reported in patients with uterine, breast and lung cancer and uterine myoma. A tendency towards lower levels of activity of the enzyme within neutrophils has also been noted in patients with carcinoma of the stomach and precancerous states of the larynx, although without statistical significance; patients with endometriosis showed elevated activity of the enzyme.

Acetylglucosaminidase↗
PubMed1984

Acid phosphatase of neutrophils in patients with malignancies.

In 132 patients suffering from various malignancies, including uterine carcinoma, breast cancer, gastric carcinoma, cancer of the colon, and additionally, patients with uterine myomas and endometriosis an increased activity of AP within the peripheral blood neutrophils could be stated by using a histochemical method. According to the author's opinion an increased activity of that enzyme reflects a response against local inflammatory processes frequently accompanying malignant tumours.

Acid Phosphatase↗
PubMed1984

Enzymes of neutrophils in workers occupationally exposed to benzene, toluene and xylene.

Histochemical studies of peripheral blood neutrophils have been performed in 106 workers occupationally exposed to benzene, toluene and xylene. The benzene, toluene and xylene concentrations in the air at work places ranged from 0-370, 0-580 and 0-560 mg/cu.m, respectively. It as been stated that the initial period of exposure is characterized by adaptative reactions of neutrophils expressed in increased intracellular activity of acid hydrolases and diminished glycogen reserve. The advanced period of exposure, that is after 55 months of exposure, is associated with some signs of decreased biological deficiency of neutrophils expressed in lowering the activity of selected enzymes and accumulation of both glycogen and lipids. According to the authors opinion the evaluation of the enzymatic content of neutrophils may be helpful in monitoring the toxic effects of benzene and its homologues in industrial workers.

Acetylglucosaminidase↗