Search PubMed⌕ Search

Biomedical subjects

J Lisiewicz

Publications and source records attributed to J Lisiewicz.

At least 181 records · Page 10Linked to original sources
PubMed1975

Beta-glucuronidase activity of lymphocytes in the premature infant with special reference to the fetal maturation stage.

Activity of beta-glucuronidase (GR) was determined cytochemically by the Hayashi et al. method on peripheral blood lymphocytes of 50 premature infants, born between 21st and 37th week gestation. The subjects studied were subtyped into group I (21st to 28th week), group II (29th to 33rd week), and group III (34th to 37th week). Absolute count of enzyme-positive lymphocytes was lower in group I, when compared with group II and III as a whole. Absolute count of GR-positive lymphocytes exhibiting granular reaction type was significantly lower in group I, when compared with other groups. Absolute counts of GR-positive cells, with both granular and diffuse, or diffuse enzymatic reaction did not differ in groups I, II and III. The results obtained indicate ontogenetic development of lysosomal granules containing GR in the lymphocyte cytoplasm in premature infants related to their fetal age.

Female↗
PubMed1975

Enzymatic equipment of lymphocyte lysosomes in the premature or term infants and in the adults.

The activity of some lymphocyte lysosomal enzymes such as: acid phosphatase, beta-glucuronidase and beta-glucosaminidase was studied comparatively in 29 prematures, 20 term infants and 20 adults. The activity of lysosomal enzymes was found to increase gradually in the course of fetal maturation and later on after birth. The percentage of lymphocytes exhibiting strong intracellular acid phosphatase and beta-glucuronidase activity was highest in adults. Differences between lymphocyte beta-glucuronidase activity in the term infants and that in adults were less evident. The results of the study seem to confirm the previous assumption of certain authors that B lymphocytes, generally characterized by a low activity of lysosomal enzymes, are more numerous in the premature and term infants than in the adults.

Acid Phosphatase↗
PubMed1975

Neutrophil alkaline phosphatase activity in the early puerperium.

Neutrophil alkaline phosphatase studied cytochemically according to Kaplow's method in 20 healthy women aged 20 to 38 years, shows statistically significant, although transient, increase on the second day after physiological delivery and returns to initial values after several days. The above increase is unrelated to the increased enzyme activity observed in the terminal months of pregnancy in a majority of healthy women. It is associated with an increase in neutrophilic granulocytosis in the puerperial period.

Adult↗
PubMed1975

Semiquantitative cytochemical determination of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase activity in peripheral blood lymphocytes.

Activity of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase in peripheral blood lymphocytes was determined cytochemically in 20 normal subjects, 10 male and 10 female, by the use of BARKA and ANDERSON's (1962), HAYASHI et al. (1964) and HAYASHI's (1965) methods, respectively. Results obtained were semiquantitatively according to subdivision of lymphocytes into enzyme-negative and enzyme-positive cells. Enzyme-positive lymphocytes were divided into cells with granular, mixed granular and diffuse enzymatic reaction type. In the first two types of cytochemical reaction a number of enzyme-positive lysosomal granules were counted and expressed in terms of both absolute count and percentage of circulating lymphocytes. Enzyme-positive lymphocytes represented 80.3%, 40.5% and 41.5% of the total lymphocyte count in regard to the presence of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase, respectively.

Acid Phosphatase↗