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Biomedical subjects

J Lindner

Publications and source records attributed to J Lindner.

At least 91 records · Page 5Linked to original sources
PubMed1982

[Morphology and biochemistry of wound healing].

Wound healing was investigated by morphological and biochemical methods. The results show that wounds cause disturbances in the equilibrium between the physiological anabolic and catabolic processes of cells, ground substance, and fibers (with increased turnover rates). The primary increase of catabolism is responsible for the secondary compensatory enhanced anabolism, especially of proteoglycans and collagen. Kind, size, and localization of wounds determine the course of wound healing and therefore the extent of adaptation of structure, function, and biomechanical capacities on the surrounding uninjured tissue.

Animals↗
PubMed1982

Connective tissue aging in the human hypophysis-gonadal system.

The basic issue in our project was to quantify the age-process in the human hypophysis-gonadal system. Of the many aspects of aging, we estimated, using human autopsy material, the content of desoxyribonucleic acid (DNA), of hydroxyproline, of hexosamines and of uronic acids in the hypophysis, testis, and ovary. The respective organs were weighed and homogenised in acid-buffer solution after which, for each of the parameters, 1.0 ml of homogenate was extracted and processed: DNA-estimation according to modified Dische/Seibert (1929) method; hydroxy-proline according to Stegemann and Stalder (1967); hexosamines and uronic acids according to Gatt and Berman (1966) and Blumenkrantz and Asboe-Hansen (1973) respectively. All values obtained were expressed in micrograms/ml of homogenate. The age investigated ranged from a few days old to 99 years. The DNA-curves for the hypophysis initially showed a decline till about the third decade of life and then remained almost constant with a significant decline up to the senile age. The DNA-curves for the testis and ovary demonstrated a sharp decline initially till the third decade of life, after which the curves remained constant, but showed reduction during higher age. Similar pattern of distribution was also observed for hexosamines, and uronic acids: after the initial decline of uronic acids in the human hypophysis and testis during the first three decades of life and nearly constant values thereafter, hexosamines, together with the uronic acids show minimal decrease in content during the senile period. Contents od hydroxyproline significantly increased in testis, ovary and hypophysis with aging. This biochemically proven age-fibrosis in the human gonads corresponds to the morphologically observed vascular and interstitial sclerosis in the testis and the ovary and may partly help to explain the declined sensitivity of these target organs to hypophyseal stimulation during the aging period.

Adolescent↗
PubMed1982

Dissociation of LH and FSH Responses to LHRH during estrogen therapy of patients with ovarian failure.

This study examines the effect of oral estrogen treatment on gonadotropin secretion in three young women with gonadal failure. Each subject was treated with 0.1 mg BID of ethinyl estradiol for four weeks, and the LH and FSH responses to 200 microgram of intravenously administered LHRH were measured basally and weekly during therapy. Significant reduction of basal levels of FSH occurred within one week of treatment, with obliteration of LHRH-mediated FSH responsiveness within two weeks. By contrast, basal levels of LH were significantly reduced by the end of the second week of treatment, and LHRH-mediated LH levels were sustained for three weeks. In one subject an LHRH test was performed every other day for two weeks after cessation of therapy. Return of FSH responsiveness was delayed one week beyond that of LH, which occurred within three days of discontinuation of estrogen. These results indicate that during the early phase of oral estrogen replacement therapy, FSH secretion may be selectively blunted; after discontinuation of treatment, recovery of FSH secretion lags behind recovery of LH.

Adult↗
PubMed1982

Changes in cell cultures of bovine corneal endothelium cells as related to donor age and number of passages in vitro.

Aging changes of the acidic glycosaminoglycans (aGAG) were investigated in bovine corneal endothelium cells grown in vitro, using histochemical methods and the determination of hexosamine and uronic acid. The time interval between trypsination and confluency of the monolayer and the cell counts at different time intervals of the same passage number served as parameters for the proliferation capacity of the cultures. A cell line, derived from the eye of a 1,5 year old cattle showed a low proliferation capacity in the first 9 passages, which increased until the 15th passage, to decrease thereafter until the 29th passage. In cells from eyes aged 11 and 12 years respectively the initial proliferation capacity was very low to reach the same values as in cultures of the young eye at the 10th passage but remained constant thereafter. Chromosome counts showed an increased number of hyperploids in late passages of cells from the aging eye with counts around 4n-8n increased to 17% as compared to 5% in the cells derived from the young eye. The cell densities were increased in cultures of high passage numbers. Histochemically the aGAG were shown in the cell nucleus, and the cytoplasm as well as in the intercellular space. In the nuclei of cells from aging eyes and in late passage cells of the young eye they showed an increased resistance against resting hyaluronidase. The hexosamine and uronic acid values were high in early passages of cultures from the young eye and decreased in late passages of the young eye as well as in early passages of cells from the aging eyes. The changes in the aGAG as well as the changes in the cell proliferation are similar comparing early passage cells of aging eyes to late passage cells of the young eyes. But the aGAG changes are more pronounced in cells of eyes aged in vivo. In the eye the proliferation of the corneal endothelium cells is inhibited. As such these cells provide a useful model to comparing in vivo-aging changes of resting cells to changes of the same cells proliferating in vitro.

Aging↗
PubMed1982

[Age-dependent changes in connective tissue].

Alterations of the various connective tissues with aging are investigated by combined morphological and biochemical methods. The aging of connective tissues depends on aging of informational- and structural-macromolecules. Aging-dependent alterations of proteoglycans/GAG and collagen can be caused by changes in genetic information or in the cellular information-coded synthesis, but also by changes in the controls of the various metabolic processes of connective tissue cells. The activities of enzymes involved in the anabolism and the catabolism of proteoglycans/GAG and collagen show reductions with aging, but do not show age-specific variations. The aging of connective tissues is rather a dynamic process (with measurable metabolic parameters of the various connective tissue cells and their products) than a passive or so-called degenerative connective tissue process. The bradytrophy concept of connective tissue cannot be accepted any longer, because connective tissue cells partly have metabolic rates at the same level as parenchymal cells. Furthermore, parenchymal cells can synthesize and degrade mesenchymal structural macromolecules. Connective tissue aging is demonstrated on 3 organ groups: 1. on typical mesenchymal organs: aorta, cartilage and skin, 2. on organs, which are mainly composed by connective tissue: heart and lung, 3. on connective tissues of parenchymal organs: liver and kidney. These various organs exhibit some common basic processes but also differences in connective tissue aging, which are due to the different composition of proteoglycans/GAG and collagen types, and on structure and function. The aging of connective tissues is of special importance for the aging of organs. Besides alterations of cells and GAG, the very important aging fibrosis in several mesenchymal and parenchymal organs is demonstrated and discussed. The connective tissue aging of organs is one of the reasons why the frequency of diseases increases with aging and diseases are grafted more strongly on aging cells, tissues, organs and organisms.

Aging↗
PubMed1981

[Correlation between lipids, especially HDL-cholesterol, and increasing alcohol consumption].

In 113 male patients different lipid parameters were investigated and relations to the daily quantity of alcohol were established. In increasing alcohol consumption we found an increase in triglycerides, and involution of the LDL-cholesterol and changing relation to the total cholesterol. There was a positive correlation between HDL-cholesterol and medium doses of alcohol, whereas an abuse of more than 100g/day led to a decrease of this parameter. One should have a very critical attitude to a vessel-protective effect of alcohol, since in most cases the regular alcohol consumption is associated with a complex of various risk factors. An alcohol prophylaxis of the arteriosclerosis is to be refused.

Adult↗
PubMed1981

Characterization of human anti-luteinizing hormone-releasing hormone (LRH) antibodies in the serum of a patient with isolated gonadotropin deficiency treated with synthetic LRH.

In this report we describe the characteristics of human anti-LRH antibodies detected in the serum of a male patient with isolated gonadotropin deficiency. He had received 90 days of therapy with LRH (1 mg, sc, three times daily) and was then placed on three cycles of intermittent therapy (3 weeks of LRH daily, followed by hCG every 3 days for 15 days). At the start of the fourth cycle of therapy with LRH, he developed urticaria at the site of injection, at sites of previous LRH injections, and at distant sites. Upon direct skin testing, the patient reacted positively to 0.02 ng LRH intradermally. A positive intradermal reaction was induced in a normal adult male by preparing his skin with 0.1 ml of the patient's serum and, 24 h later, injecting 0.2 microgram LRH at that site. A binding factor for LRH was detected in the patient's serum by incubation with [125I]LRH. The serum bound 33% of tracer compared to 6% in control serum. We have detected both immunoglobulin G and immunoglobulin E antibodies against LRH in the patient's serum. We have compared displacement of tracer by synthetic LRH with displacement achieved by a series of analogs. Displacements of tracer by LRH, [Lys8]LRH, [D-Trp6,Pro9-NEt]LRH, [des-Gly10]LRH, and [Phe2]LRH were similar, whereas the potencies of Ac-LRH5-10 and AcLRH2-10 were 0.1% or less.

Adult↗
PubMed1981

[The significance of arthrography and arthroscopy in the diagnosis of meniscus injury--a comparative study (author's transl)].

The article reports on 364 double-contrast arthrographics and 185 arthroscopies. The results obtained in 126 patients in whom both arthrography and arthroscopy had been conducted, were compared. It became evident that arthrography is of high informative value in the diagnosis of lesions of the meniscus, so that preference may well be given to this method in non-specific knee-joint complaints where meniscopathy is suspected. Arthroscopies are indicated in cases of clinico-arthrographic doubt. Trial arthrotomy for clarifying doubtful meniscus lesions without previous exploitation of all arthrographic/arthroscopic possibilities is no longer justified and should be abandoned. Arthroscopy is definitely superior to arthrography in the diagnosis of, in particular, cartilage structures, of the synovia and of the retropatellar space. It is here where arthrography has its narrow limitations--now and in the future--for methodical reasons.

Adolescent↗
PubMed1981

[Changes in total beta-glucuronidase activity and DNA content of various tissues and organs of newborn rats after prenatal administration of glucocorticoid].

Quantitative biochemical analyses of the beta-glucuronidase total activity and the total DNA-content carried out on selected parenchymal and mesenchymal organs of 455 Wistar inbred rats (strain Chbb:THOM (SPF) from 56 litters (untreated or treated with 1 mg betamethasone/kg b.w. s.c. on both the 18th and 19th days of pregnancy, applied to the mother animal) led to the following results: The DNA-content of lung, liver, kidney, skin and rib cartilage declined continuously both in the controls and in the treated group during the postnatal observation period (from the 1st to the 15th day p.p.). The prenatal glucocorticoid administration led to a decline in the DNA-content in comparison to the untreated controls, especially evident in the first postnatal days. The beta-glucuronidase activity of lung, liver and kidney showed an increase in the postnatal development phase to a maximum occurring organ-dependently on different days with a decrease afterwards. Due to glucocorticoid-pretreatment of the mother animals the newborn rats showed significantly higher enzyme values within the first postnatal days than did the untreated controls. Between the 6th and 9th day (depending on the organ) the same beta-glucuronidase activities could be found on pretreated and untreated animals. For the further ages tested in this study (till the 15th day of postnatal development) the enzyme activity of the experimentally treated litters was lower than that of the untreated controls. In connection with related findings, especially regarding morphological changes in the lung, the induction of beta-glucuronidase as well as the decrease in the DNA-content due to prenatal glucocorticoid pretreatment are discussed in terms of a maturation acceleration.

Aging↗
PubMed1981

[3H-Thymidine autoradiographic studies on proliferation of various smooth muscle cell populations pre- and postnatally (and its possible modification) (author's transl)].

The pre- and postnatal proliferative kinetics of vascular and gastrointestinal smooth muscle cells (and possible influences on it) are reported by means of their 3-thymidine labelling indices. The 3H-autoradiographic investigations were carried out on Wistar inbred rats, strain Chbb:THOM (SPF), and on genetic osteoarthrosis mice, strain C 57 black St. Louis (in vivo investigations). The results show that the smooth muscle cells of the colon have significantly higher 3H-thymidine labelling indices than the smooth muscle cels of the other parts of the gastro-intestinal tract. Prenatally the label indices were 40 times higher than during postnatal maturation. The 3H-thymidine labelling indices (as a parameter of cell proliferation) of fetal cells of the vascular wall as well as the smooth muscle cells of the aorta during postnatal maturation are just about 10% the values of gastrointestinal smooth muscle cells at the same time. Moreover, the fetal cells of the vascular wall show 10 times higher values for the 3H-thymidine labelling index than do the smooth muscle cells of the aorta during postnatal maturation. The finding that smooth muscle cells of the vascular wall have low proliferation rates and reach a stable state later than many other populations of smooth muscle cells is discussed with regard to its theoretical and practical-therapeutical importance using kallikrein for a drug example.

Aging↗
PubMed1980

[Changes in skin DNA in newborn rats due to betamethasone in the last phase of pregnancy (author's transl)].

In an extensive series of experimental studies the DNA content of the skin of pure Wistar inbred rats was investigated to see whether any change occurred in the skin DNA content when the dams were pretreated with 1 mg betamethasone/kg b.w. s.c. on the 18th and 19th days of pregnancy. The newborn rats (age: 1, 2, 4, 9, 12, 15 days old) were sacrificed and the dams treated between 7.30 and 8.30 a.m. in order to avoid possible variations of the results due to the diurnal cycle. During the observation period there was a linear decline of DNA content between 1-day and 15-day old rats. This true for the control group as well as the treated animals. The finding that the DNA content in this tissue dropped in the first few days of life as a result of prenatal glucocorticoid application is of great importance.

Animals↗