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Biomedical subjects

J Lindner

Publications and source records attributed to J Lindner.

201 records · Page 12Linked to original sources
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[Age dependence of the early reaction in glycosaminoglycan metabolism of the rat liver on damage following a single dose of thioacetamide].

Wistar rats aged 7 or 22 months resp. were treated with 50 mg/kg of thioacetamide (TAA) i.p. The TAA-treated animals as well as control groups of the same ages and containing the same number of animals were injected i.p. with 3H-glucosamine (0.2 microCi/g body weight) 2 hours before sacrifice. Previously to sacrifice, the livers had been perfused in narcosis with NaCl (0.9%). Following degradation of the tissue by pronase, specimen of the substances precipitable by ethanol were digested with chondroitinases, streptomyces-hyaluronidase, keratanase, and nitrite. The quantities of substances were estimated photometrically using Alcian blue; radiometry was performed following precipitation with cetylpyridiniumchloride. In aging animals the amount of dermatan-sulphate (DS) and of the total acidic glycosaminoglycans (a.GAG) is increased as compared to the younger animals, although there ist no age related difference in the incorporation of glucosamine. The incorporation per microgram a.GAG (spec. act.) is decreased. Following TAA the amount of DS is increased in both age groups, whereas the total amount of GAG is increased only in the younger animals. The incorporation per mg dry weight is also increased in all GAG types of both age groups which is also the case with the spec. act. (with the exception of HS in the younger animals). The increase of the incorporation per mg dry weight in DS is significantly lower in the older TAA-animals as compared to the younger ones. HS shows the same tendency, but there is no significance. The age related increase of hydroxyproline parallels that of DS.(ABSTRACT TRUNCATED AT 250 WORDS)

Acetamides↗
PubMedDate not supplied

Body weights, absolute and relative organ weights by maturation and ageing (with sexual differences), and their importance as measures of reference for metabolic investigations.

In this contribution, several organs important, especially for geronto-pharmacological not only out of theoretical but especially for practical clinical reasons, to the development of absolute and relative organ weights of rats are examined, as related to a) body weight that is exhibited during the life span, and b) to the weight of the brain. This examination deals with male and female albino rats of the Sprague-Dawley-inbred strain, Sut : SDT ( Chbb : SPF) of both sexes that were two months old at the beginning of the experiment. The experiment results are given in monthly intervals and should be reference figures for metabolism and performance analyses of the examined organs during the course of life into old age (in continuation of corresponding earlier studies, also with humans). Since the following results are obviously of great importance to human medicine, the corresponding age groups for comparison are given and verified. The findings also show that pharmacokinetic experiments in extended test series concerning development during the course of life, therefore concerning all age groups, with the use of the here tested parameters, are better to conduct with female rats than with male. Along with this, the relative organ weights in relation to the brain weight are better to use than the (until now, usual) relative organ weights in relation to body weight. Further shown by the results of these studies is that, during the often necessarily large series, it can be preferable to use the absolute organ weights rather than the relative organ weights (as related to body weight).(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗