Biomedical subjects

J Lindhe

Publications and source records attributed to J Lindhe.

At least 271 records · Page 15Linked to original sources

PubMed1975

Effect of chlorhexidine on gingival wound healing in the dog. A histometric study.

Wound healing after gingivectomy was studied in five 1-year-old dogs (Beagles). During a preparatory period of 4 months, the teeth of the dogs were brushed twice a day using a toothbrush and dentifrice. At the end of this period, all gingival units appeared healthy. All mechanical tooth cleaning procedures were then terminated. Following a period a 2 weeks without oral hygiene, six gingival units, taken bilaterally from the maxillary first molar and posterior premolar areas, were subjected to standardized gingivectomies. Subsequently, the biopsied areas of the left half of the jaws were treated twice a day with a 0.2% aqueous solution of chlorhexidine digluconate for 42 days, while corresponding areas of the right jaws were given saline treatment. Tissue specimens were obtained after healing periods of 42, 28, 14, 7, 4 and 2 days. They were then immediately placed in a Karnovsky fixative, and afterwards decalcified in EDTA. The rate of tissue regeneration and degree of inflammatory cell infiltration were evaluated with histometric methods. It was found that while gingival wounds treated with saline regenerated with extensive inflammatory reactions, corresponding chlorhexidine-treated wounds healed with only minor signs of inflammation.

Animals↗

PubMed1975

Effect of proper oral hygiene on gingivitis and dental caries in Swedish schoolchildren.

The present paper reports the effect of a 3-year study of regularly repeated professional toothcleaning on oral hygiene status, gingivitis, and dental caries. At the initiation of the study in 1971, the children were 7-8 years old (Group 1), 10-11 (Group 2) or 13-14 (Group 3). The children were divided into test and control groups. Before the study all the children were examined regarding oral hygiene, state of the gingiva and dental caries. Throughout the 3-year period the controls brushed their teeth with a 0.2% sodium fluoride solution once a month under supervision. During the first 2 years of the trial all the children of the test groups were given professional oral prophylaxis once every second week during the school terms. During the third year the interval between consecutive prophylactic sessions was prolonged to 4 weeks in Groups 1 and 2 and to 8 weeks in Group 3. The results demonstrated that regularly repeated professional cleaning of the teeth combined with fluoride applications and toothbrushing instructions, over a 3-year period in schoolchildren resulted in the establishment and maintenance of excellent oral hygiene standards. Inflammation of the marginal gingiva almost entirely disappeared, and practically no new carious lesions developed.

Adolescent↗

PubMed1975

Effect of fluoride on gingivitis and dental caries in a preventive program based on plaque control.

The purpose of the investigation was to estimate the relative effect of fluoride on caries in a preventive program based on meticulous plaque control. The material consisted of 82 children; 41 in the test and 41 in the control group. At the start of the study the children were 13-14 years of age. In August 1973 an examination was performed to obtain baseline data for the trial. Prophylactic treatment was given to the children once every second week. A total of 18 treatments were given during a 10-month period. In the control group an abrasive paste was used containing 5% sodium monofluorophosphate (Jodka Fluor Polerpasta). In the test group an abrasive paste was used which was identical with the control paste except that it did not contain any fluoride components. Twelve months after the baseline examination the test and control groups were reexamined. The trial demonstrated that, in children, fortnightly professional cleaning of the teeth combined with meticulous toothbrushing instructions result in (1) a high standard of oral hygiene and (2) only very few new carious surfaces. Though the control group children received fortnightly topical application of F- during the prophylactic sessions and used a fluoridated dentifrice, there was no significant difference between the two groups regarding number of new carious tooth surfaces per child per year.

Adolescent↗

PubMed1975

Effect of listerine on dental plaque and gingivitis.

The present study was performed in 10 adults in order to evaluate the effect of an antiseptic mouthrinse (Listerine) on the rate of dental plaque formation and gingivitis development during a 2-week period when all efforts towards active mechanical oral hygiene were withdrawn. The study was performed as a crossover study and was carried out during four consecutive 2-week periods. During the first and third periods (preparatory periods) the participants were subjected to repeated professional tooth cleanings in order to establish plaque- and gingivitis-free dentitions. During the second and fourth periods (test and control periods) the participants were not allowed to brush their teeth but rinsed their mouths three times a day with Listerine or a placebo mouthwash. Plaque Index, Gingival Index, gingival fluid flow, and crevicular leukocytes were assessed on d 0, 2, 4, 7, and 14. On d 7 and 14, dental plaque was removed from the right and left jaws respectively and the wet weights determined. The chemotactic activity elaborated by the plaques was studied in Boyden chambers. During the Listerine test period, significantly lower Plaque and Gingival Index values were scored and lower amounts of plaque could be sampled in comparison to the control period.

Adult↗

PubMed1975

Experimental gingivitis in young dogs.

The aim of the present study was to analyze and express in quantitative terms some of the structural alterations which develop in an initially normal gingiva during a phase of continuous plaque accumulation. Four beagle dogs were used. The animals had from birth been twice daily subjected to meticulous mechanical tooth cleaning. When the dogs were 10 months of age their gingiva were in excellent health as evaluated by Gingival Index and Gingival Exudate measurements. Gingival tissues were harvested from the premolar and molar regions in the right jaws. The tooth cleanings were then terminated and plaque allowed to accumulate. Clinical examinations were performed and gingival biopsies sampled after 4, 7, 14, 21 and 28 d. The composition of the gingival biopsies was analyzed in a sampling microscope. After 4 d of plaque accumulation significant amounts of gingival exudate could be sampled. The exudation then gradually increased during the following weeks. Biopsies representing day zero did not contain any inflammatory cell infiltrates. However, after 4 d of the experiment leukocytes were found in the collagen-poor connective tissue immediately beneath the junctional epithelium. The size of the infiltrated connective tissue (ICT) gradually increased during the experiment. The volumetric density of collagen in the noninfiltrated connective tissue (NCT) was always much higher than in ICT. In ICT, however, this density parameter remained rather constant throughout the study. On days 4 and 7 neutrophilic granulocytes constituted 60-70% of the leukocyte population. On day 28, however, the infiltrate comprised mainly mononuclear leukocytes, especially plasma cells, neutrophils at that time occupying only a small fraction of the infiltrate.

Animals↗

PubMed1975

Effect of soluble plaque factors on inflammatory reactions in the dental pulp.

The present investigation was undertaken to determine whether soluble factors from human dental plaque are capable of inducing or mediating pathological alterations in the dental pulp. The experiment was performed in three monkeys and involved 40 teeth in all: 20 test and 20 control teeth. Class V cavities were prepared on the buccal surface close to the marginal gingiva. In the test cavities an extract of human dental plaque was applied once very 5 min during an 8-h period. The cavities of the contralateral teeth were treated in an identical manner but with Ringer's solution. The animals were sacrificed 10 or 32 h after the initiation of the test. Two hours prior to sacrifice the animals were injected with colloidal carbon and then perfused with 10% neutral buffered formalin. Histologic examination demonstrates that the pulp tissue response to topical application of a plaque extract was characterized by (1) increased degree of carbon retention - indicative of vascular exudation and (2) enhanced rate of emigration of neutrophils and monocytes.

Animals↗

PubMed1975

Experimental periodontal breakdown in the dog.

This paper describes a method for inducing rapidly progressing and reproducible periodontal lesions around selected teeth in dogs. The experiments were performed on 10 beagles which were fed a diet favoring gross formation of plaque. Approximately 1 mm of the marginal alveolar bone aroumd 4P and P4 was removed and a notch prepared in the root. A ligature of cotton floss was placed around 4P and P4 at the cemento-enamel junction. Radiographs and sections prepared 230 d later revealed that a progressive breakdown of the periodontal tissues had occurred. The average bone loss was 2.4 mm and the distance between the apical cells of the pocket epithelium and the notch was 854 mum.

Animals↗

PubMed1975