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Biomedical subjects

J Lindeman

Publications and source records attributed to J Lindeman.

104 records · Page 6Linked to original sources
PubMed1977

The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.

The influence of different fixation methods on the results of immunoperoxydase staining of immunoglobulin and gastrin producing cells in gastric and duodenal mucosa was investigated. An indirect method was used on paraffin sections. It appeared that that fixatives containing sublimate gave the most consistent results, a sublimate-formaldehyde mixture being the best.

Fixatives↗
PubMed1977

The immunoglobulin-bearing cells in the lamina propria and the clinical response to a gluten-free diet in dermatitis herpetiformis.

In 17 patients with DH, multiple duodenal and jejunal biopsies were performed. In all patients the small-intestinal biopsy-specimens showed histopathological changes compatible with coeliac disease. Fourteen of the patients maintained a gluten-free diet (GFD) for more than 8 months. The small-intestinal lesions improved in all patients investigated during the GFD. The dosage of Dapsone needed to control the sin lesions could be reduced by more than 50% in 4 patients and the Dapsone could be stopped in 5 other patients on GFD. The immunoglobulin-bearing cells in the lamina propria were counted in 8 patients not on a gluten-free diet, in 6 patients on gluten-free diet, and in 8 healthy controls. The numbers of IgA-, IgM- and IgG-bearing cells were increased in most of the DH patients who were not on a gluten-free diet. The number of IgM-bearing cells in the DH patients who were on a gluten-free diet was the same as that in the control group. This may indicate a mainly IgM response in the lamina propria induced by gluten.

Adult↗
PubMed1976

Use of immunohistochemical and morphologic methods for the identification of human growth hormone-producing pituitary adenomas.

Indirect immunoperoxidase and immunofluorescent techniques were used for the detection of growth hormone in routinely processed human pituitary adenomas. With the immunoperoxidase method, distinct immunoreactive cells could be demonstrated in 15 adenomas; all except for one were associated with acromegaly. Immunofluorescence was less useful because of autofluorescence due to the fixation. In histologic stainings, the immunoreactive cells were acidophyl. With immunohistochemistry and electron microscopy, densely granulated and sparsely granulated adenomas could be distinguished. Sparse granulation was associated with an expanded rough-surfaced endoplasmic reticulum and Golgi complex. Clinically, the sparsely granulated adenomas were characterized both by active hormone secretion and aggressive local growth. It is concluded that the immunoperosidase method is of value for functional classification of pituitary adenomas. For information about secretory activity we suggest that electron micrographs of adenoma cells be examined.

Adenoma↗
PubMed1976

Rat liver macrophages will not phagocytose fibrin during disseminated intravascular coagulation.

Disseminated intravascular coagulation was induced in rats by injection of a silver colloid suspension or thrombin. Ten min after the injection of colloid, fibrin deposits were observed light microscopically in all major organs. At 30 min, fibrin was no longer present. In rats treated with antifibrinolytics (epsilon-aminocaproic acid or Trasylol) fibrin was still present at 30 and 60 min. Interaction of fibrin with Kupffer cells was studied by electron microscopy. At 3, 10, and 20 min after the colloid injection, all fibrin occurred extracellularly, close to the surface of Kupffer cells. At 30 min, all fibrin had disappeared. In rats pretreated with antifibrinolytics, too, all fibrin was found extracellularly at 10, 30, and 60 min. Comparable results were obtained when thrombin was used to induce coagulation. It is concluded that removal of native fibrin from the circulation by Kupffer-cell phagocytosis is unlikely.

Animals↗
PubMed1975

A modified method for tissue localization of cells bearing a complement receptor.

A modification of the method originally described by Dukor et al. (1970) was used for localization of cells bearing a complement receptor in lymphoid tissue of man, guinea pig, mouse and rat. Optimal fixation of the adherent indicator erythrocytes to the tissue section and satisfactory morphology of the tissue were obtained with a mixture of 1% paraformaldehyde and 0.5% glutaraldehyde. In addition, selective staining of indicator erythrocyte hemoglobin with Amido Black resulted in high contrast with the tissue constituents. The combination of fixative and stain descirbed above permitted more precise localization of cells bearing complement receptors in tissue sections.

Amido Black↗
PubMed1975

Fibrinolytic activity in aneurysmal bone cysts.

Euglobulin fibrinolytic activity of cyst fluid from six patients with aneurysmal bone cysys was considerably higher than that of arterial and venous blood of the corresponding patients. The high fibrinolytic activity was associated with a very low concentration of fibrinogen and a low concentration of plasminogen. Correspondingly, a high plasminogen activator activity was found in cyst tissue related to endothelial lining. It is suggested that fibrinolysis is an important factor in the maintenance and expansion of the aneurysmal bone cyst.

Adolescent↗
PubMedDate not supplied

Carcinoembryonic antigen in sputum cytology.

Carcinoembryonic antigen (CEA) activity was studied in exfoliated cells in sputum from patients with carcinoma of the lung and its precursor lesions. For this purpose, immunohistochemical techniques were applied on paraffin-embedded cell blocks of sputum. In the cancer patients, not only the morphologically malignant cells were CEA positive, but squamous cells that lacked the cytologic features of malignancy also were CEA positive. Some of these CEA-positive cells originated in morphologically benign squamous epithelium. In high-risk patients without clinical evidence of neoplasm, some benign squamous cells were CEA positive, possibly indicating the presence of an undetected or developing cancer.

Adenocarcinoma↗