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Biomedical subjects

J Lesko

Publications and source records attributed to J Lesko.

33 records · Page 2Linked to original sources

[Problems of hemorrhage in the cerebellum].

The authors give an account on their experience with the treatment of five patients (three women and two men) with non-traumatic haemorrhage into the cerebellum. The oldest patient was 67 years, the youngest 14 years, the mean age was 51.2 years. In four the cause of haemorrhage was hypertension, in one patient an A-V malformation. The correct diagnosis was established by CT examination, while originally it was assumed that a supratentorial cerebrovascular attack was involved. One female patient recovered after conservative treatment, in the remainder the haematoma was removed by suboccipital craniectomy. One patient died, the fourteen-year-old female patient which was admitted with symptoms of decerebration, survives with a marked neurological deficit, three patients feel well. In one instance hydrocephalus developed which was treated by ventriculo-peritoneal drainage with a valve.

Adolescent↗

A simple novel procedure for preparation of herpes simplex virus subunit vaccine.

A simple procedure for preparation of herpes simplex virus type 1 (HSV-1) subunit vaccine is described. The method is based on treatment of virus-infected cells with a nonionic detergent, removal of cell nuclei by low speed centrifugation, separation of viral nucleocapsids by high speed centrifugation through a sucrose cushion and, finally, precipitation of viral and cellular glycoproteins and proteins with ammonium sulphate (AS). Unlike to acetone precipitation, affinity chromatography on lectins or hydroxylapatite chromatography, AS precipitation repeatedly yielded the best and most reliable results as judged by relative protein content, antigenicity and immunogenicity of the final vaccine product.

Ammonium Sulfate↗

Discrimination of human placental alkaline phosphatase allelic variants by monoclonal antibodies.

Eighteen monoclonal antibodies were produced by the mouse hybridoma method using purified placental alkaline phosphatase (ALP) as antigen. The ability of the various antibodies to discriminate among allelic variants of the enzyme was tested using a large panel of placental ALPs that had been typed electrophoretically. The panel included sets of samples of each of the six common polymorphic phenotypes as well as a series of rare variants. The reactivity of each antibody with each placental ALP (binding ratio) was determined relative to a single standard placental ALP (type 1) in a quantitative binding assay. The findings for six of the antibodies have already been reported. The results on the other 12 antibodies are presented here, and the combined data on the total series of 18 antibodies are analyzed and discussed. Six of the 18 antibodies showed significantly reduced binding to one or another of the products of the three common alleles. In three cases, the discrimination was reflected by essentially "all-or-none" binding reactions. In the other three cases, the binding differences were less marked but could be demonstrated by quantitative comparisons of the binding ratios. Quantitative binding ratio comparisons also enabled heterozygotes to be differentiated from homozygotes in each case. Some of the antibodies showed reduced binding with certain of the rare variant ALP electrophoretic phenotypes. It is estimated that at a minimum this unselected series of 18 antibodies is directed to at least nine different antigenic determinants on the surface of the placental ALP molecule. The results illustrate the power of monoclonal antibodies to discriminate among allelic variants of enzymes.

Alkaline Phosphatase↗