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Biomedical subjects

J Lenard

Publications and source records attributed to J Lenard.

86 records · Page 5Linked to original sources
PubMed1971

Spin-labeled electron spin resonance study of the lipid-containing membrane of influenza virus.

The organization of the lipid-containing membrane of influenza virus has been studied by the use of three different lipid spin labels, and the results are compared with a parallel study on human erythrocyte ghosts. The lipid phase of the viral membrane is slightly more rigid than that of the erythrocyte ghosts. The data suggest that the viral lipid is arranged in a bilayer. The data suggest that the viral lipid is arranged in a bilayer. The glycoprotein spikes covering the viral membrane were specifically removed by proteases, and no alteration in the environment of any of the three spin labels was detected. This suggests that the spikes are not involved in determining the organization of the lipid bilayer.

Cell Membrane↗
PubMed1968

Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

The effects of several commonly employed fixatives on the three-dimensional conformations of two soluble proteins and the protein of intact red blood cell membranes have been studied by means of circular dichroism measurements in the spectral region of the peptide absorption bands. The fixatives used produced significant and parallel conformational changes in all of the proteins, in the increasing order: glutaraldehyde; OsO(4); glutaraldehyde followed by OsO(4); and KMnO(4). The last two treatments obliterated most of the helical character of the proteins. The significance of these observations to the preparation of specimens for electron microscopy is discussed.

Animals↗