Biomedical subjects
Publications and source records attributed to J Leclerc.
During lactation a methionine supplemented low protein diet or a sufficient protein diet containing 40% of fructose induced an important hepatic lipidosis. Inositol supplementation, but not choline supplementation, strongly reduced, in the case of a low protein diet, and suppressed, in the case of a sufficient protein diet, this lipidosis.
In the lactating rat, a low protein diet (8% casein) decreases strongly liver microsomal delta 6 and delta 5 desaturase activities. The supplementation of the diet with 0.4% methionine improves partly delta 6 desaturase activity but has no effect on delta 5 desaturase activity.
Mean inspiratory nasal resistances and mean axillary sweating rates were recorded bilaterally every 30 minutes for a 5-7 hour period in seven subjects. One subject performed the experiment twice. Nasal resistance was measured using either anterior (n = 5) or posterior (n = 2) rhinomanometry and sweating rate was determined using a filter paper technique. The results for seven of the experiments showed that both nasal resistance and axillary sweating rate exhibited periodic reversals in side dominance. The cyclic patterns for nasal resistance and sweating rate were synchronized in phase in two experiments and 180 degrees out of phase in five experiments. These results support the concept of a central governing cycle with peripheral expression effected by the autonomic nervous system.
Six groups of female rats which gave birth to offspring of 8 young (or adjusted to 8) were fed diets containing 2, 4, 6, 8, 12, or 16 mg of riboflavin per kg of dry matter. Seventeen days after birth, young were withdrawn from the mothers and these were placed in metabolic cages and excreta (urine and feces) were daily collected for 17 days. The activity coefficient of blood glutathione reductase was 1.45 in 17 days old young of group 2 whereas it was 1.12 in young of other groups. The coefficient was 1.09 in female rats at the end of the experiment. 5 and 6 days after excreta collection began urinary excretion of riboflavin reached a peak in females of groups 4 to 16. At the end of the experiment, females which were fed 8 mg of riboflavin/kg or more had an urinary excretion of the vitamin which reached a plateau at 50 micrograms/day. On the other hand fecal excretion of riboflavin markedly increased as the intake did. Data suggested that the intestinal absorption of riboflavin is limited but also that the requirement of riboflavin in lactating rats is 8 mg/kg of diet whereas it is 2 mg/kg for maintenance.
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A case is reported of a female patient with subjective tinnitus synchronous with her pulse, in the left ear. A dehiscent high seated jugular vein was found in the middle ear. A surgical technique is presented using a septal cartilage homograft to cover the dehiscent vein.
Dietary addition of methionine suppresses the liver lipidosis in gestating rat fed a diet containing 9% by weight of casein. Riboflavin metabolism is not affected by this addition neither in the dam nor in the newborn rat. Riboflavin content in liver increases in the dam along with the protein content of the diet but does not change in the newborn rat.
A decrease from 18 to 9% of the dietary casein level induces lower food intake in the gestating female rat and weight loss in the newborn rat. Addition of methionine to the diet containing 9% casein improves food intake in the last period of pregnancy. Body weight of the newborn rat is also increased and becomes identical to that of rats born from mothers fed 18% dietary casein.
The increased sensitivity to Bordetella pertussis of a line of CD-1 mice used in toxicity-testing and the consequent and needless rejection of several lots of DPT vaccine are described. Apart from changes in the mice the possible presence of an infective agent in the animal house could not be discounted. The usefulness of individual mouse weights in the assay as opposed to group weights and of a reference preparation to monitor the performance of mice is discussed.
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Explore the source record for details and available documents.
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We have studied the effects of oral contraceptives on three glycoproteins, alpha-1-antitrypsin (A1AT), gamma-glutamyltransferase (GGT) and alkaline phosphatase (AP), in terms of age (20--40 years), duration of administration, and levels of estrogens. We have confirmed that oral contraceptives increase the concentration of A1AT and the activity of GGT and decrease the activity of AP, but that the modifications are less pronounced with pills containing low levels of estrogens for GGT and AP. We observed a decrease in A1AT and GGT after 2 and 5 years of treatment, respectively, but an increase in AP. Patient age seemed to have little influence on the change in AP activity and A1AT concentration but GGT activity was higher for the 25--30 year group.
Decreased riboflavin concentrations were again found in different tissues from old rats (23 months of age) as compared to younger animals (11 months of age). This decrease was observed in the liver, heart and brain (-7 to -10%) and mainly in the kidneys (-60%). In the first three organs it is due to a decrease in flavin adenine dinucleotide (FAD), whereas most of the time the flavin mononucleotide and free riboflavin (FMN R) contents remained unchanged. In the kidneys, both FAD and FMN R are decreased, the latter more so than the former. This phenomenon could be explained by a modification in the enzymatic activities involved in the riboflavin FMN-FAD metabolic pathways and would result from a decrease of thyroid activity during aging. In the spleen the total riboflavin concentration is increased in old rats without changes in the relative ratios of FAD and FMN R. No particular effect was observed in lung and muscle tissue during aging.
Seven groups of female rats which had given birth to the litters of 8 youngs (or litters adjusted to 8 youngs) were fed diets containing respectively 0.25, 0.5, 1, 2, 4, 8 or 16 mg/kg of vitamin B6. Fourteen days after littering, the vitamin B6 content was measured in female tissues (blood, liver, carcass) or in those of the youngs (liver, carcass). Besides, the vitamin B6 content in the stomach of the youngs was measured, 3, 7 and 14 days after birth. 1. When the dietary vitamin B6 content is less than or equal to 1 mg/kg the blood vitamin level in the mother remains very low. As the dietary level rises (beyond 1 mg/kg) the blood vitamin level markedly increases. However the liver and carcass vitamin contents in the mother do not show any baseline value only the carcass content reaches a top value. 2. The highest vitamin B6 content in the stomach of the youngs (milk) is already reached when the diets contains 4 mg/kg of the vitamin. 3. Same results are obtained regarding the vitamin contents of the liver and carcass in the youngs. Therefore it appears that these levels are mainly related to the milk vitamin B6 content in the mother. In a previous work however, it was shown that in similar experimental conditions, the maximum tissue thiamin contents depend upon other mechanisms.