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Biomedical subjects

J Lawrence

Publications and source records attributed to J Lawrence.

At least 181 records · Page 10Linked to original sources
PubMed1981

Controlled comparison of the effects of furosemide and hydrochlorothiazide added to propranolol in the treatment of hypertension.

Forty patients completed a double-blind parallel group study comparing furosemide (FUR) and hydrochlorothiazide (HCT) when added to a stable dose of beta blocker in the treatment of mild to moderate hypertension. Both diuretics caused a significant additional fall in blood pressure (BP) when added to propranolol, and there were no differences in the mean BP achieved. However, a higher proportion of patients achieved satisfactory control (BP less than 160/95 mm Hg) on FUR than on HCT and, in addition, there was a more marked dose-response effect with FUR. This study showed that FUR is at least as effective as HCT in the treatment of hypertension when added to propranolol, and appears to possess certain advantages in comparison to the thiazide.

Adult↗
PubMed1981

On the preservation of contractile proteins during storage of human platelets.

A study has been undertaken to determine the rate at which stored platelets lose their ability to respond to stimuli and to establish whether this decrease in function could be ascribed to the storage-induced proteolysis of prominent platelet proteins observed by others. Platelet concentrates were stored at 4 degrees C and 25 degrees C for up to 14 days, and their ability to secrete and aggregate in response to appropriate stimuli was determined at 6, 96, and 192 hr after venipuncture. At each time point the protein complement of the platelets was also monitored by SDS-polyacrylamide gel electrophoresis to assess the extent of intracellular protein degradation. Platelets from concentrates stored at either temperature exhibited a decreased ability to respond to stimuli as storage time increased. After 8 days of storage at 4 degrees C and up to 9 days at 25 degrees C, no proteolysis of major platelet proteins was observed; however, complete loss of platelet function was observed. This strongly indicates that a decrease in platelet function should not be causally linked to degraded contractile-structural proteins and that extending the functional life of platelets during storage is still an attainable goal since proteolysis is not the inevitable result of short-term storage.

Binding Sites↗
PubMed1978

Chromosomal localization of human beta globin gene on human chromosome 11 in somatic cell hybrids.

We have successfully used a DNA.cDNA molecular hybridization assay to directly determine the presence or absence of human beta globin gene sequences in 20 human-mouse somatic cell hybrids, each of which contained a different subset of human chromosomes. The assay is specific for the individual human globin genes and will detect the presence of a globin gene if the relevant chromosome is present in only 10% of the cells of a hybrid population. The content of human chromosomes in each hybrid clone was characterized by Giemsa 11 staining, Giemsa trypsin-Hoechst 33258 staining, and by the use of 22 independent isozyme markers for 17 different human chromosomes. All human chromosomes were present in one or more cell lines devoid of the human beta globin gene except for 6, 8, 9, 11, and 13. Among these latter chromosomes, only chromosome 11 was present in the six hybrid clones that contained the human beta globin gene. In fact, chromosome 11 was the only human chromosome that was present in all of the six hybrid clones found to be positive for the human beta globin gene. Two sister clones, 157-BNPT-1 and 157-BNPT-4, had similar subsets of human chromosomes except that 11 was present only in 157-BNPT-4. 157-BNPT-4 contained the human beta globin gene while 157-BNPT-1 did not. DNA from three hybrid lines was also annealed to purified human gamma globin cDNA; two lines positive for human beta globin gene sequences also contained human gamma globin gene sequences while one line was negative for both beta and gamma gene sequences. On the basis of these results, the human beta and gamma globin genes have been assigned to human chromosome 11.

Chromosomes, Human, 6-12 and X↗
PubMed1978

Observations on the pathology of canine microsporidiosis.

The available literature on canine microsporidiosis indicates that this disease, primarily of young dogs, is a distinct clinicopathological entity. It has been confused with canine distemper and rabies, and must be differentiated from toxoplasmosis. Information available on the spectrum of pathological change associated with this disease is incomplete but a distinct pattern emerges from a study of the reports. The aetiological agent appears to have a predilection for the central nervous system and kidneys, but other tissues and organs, and especially the liver, may also be infected. Vasculitis and perivasculitis, which may include fibrinoid necrosis, seem to be a basic lesion. Cellular inflammation ranges from polymorphonuclear leukocyte infiltration in areas of necrosis to focal granulomas. There may be no cellular reaction to compact groups of organisms. Histopathological and ultrastructural studies of this case augment our knowledge of the pathological changes seen with canine microsporidiosis.

Animals↗
PubMed1977

Ozone in drinking water treatment: a review.

The paper reviews the application of ozone for the treatment of potable water. The period covered by the review is 1937--1975. A complete bibliography is available from the authors upon request.

Chlorine↗
PubMed1975

Neonatal secretion of secretin.

The plasma levels of secretin have been measured in mothers after labour, and in their babies at birth and on day 4 of life. The mean cord venous level was higher than the maternal level, and there was a significant correlation between the individual maternal and cord values. The level had again increased by day 4, and at this time the secretin level was inversely proportional to the blood glucose level.

Blood↗
PubMed1974

Biosynthesis of ubiquinone in Escherichia coli K-12: biochemical and genetic characterization of a mutant unable to convert chorismate into 4-hydroxybenzoate.

A mutant strain of Escherichia coli unable to carry out the first specific reaction of ubiquinone biosynthesis, that is the conversion of chorismate into 4-hydroxybenzoate, has been isolated. The gene concerned maps at about minute 79 on the E. coli chromosome and has been designated ubiC. This gene is probably the structural gene for chorismate lyase since cell extracts from a transductant strain carrying the ubiC437 mutant allele are unable to convert chorismate into 4-hydroxybenzoate and growing cells of the mutant do not form appreciable quantities of ubiquinone unless 4-hydroxybenzoate is added to the growth medium.

Anthranilate Synthase↗