Biomedical subjects

J Lasovský

Publications and source records attributed to J Lasovský.

3 recordsLinked to original sources

PubMed1998

[Fluorescence methods for studying cell damage].

We have been studying some properties of the new generation photosensitizers--phthalocyanines. The influence of the certain phthalocyanine concentrations at combination with laser irradiation doses (semiconductor laser, lambda = 670 nm, P = 50 mW) have been studied by in vitro fluorescent methods. Cytotoxicity assay LIVE/DEAD for fluorescence microscopy and measurements of cells viability with multi-well plate scanner was used. The optimal phototoxic effect on MCF7--mamma carcinoma cells was observed 8 micrograms/ml ZnPcS2, 10 micrograms/ml ClAlPcS2 in combination with laser irradiation 50 J/cm2 and 100 J/cm2 respectively. This concentration and dose killed MCF7 carcinoma cells.

Breast Neoplasms↗

PubMed1989

Electrochemical and thermal studies of some quaternary benzo(c) phenanthridine alkaloids.

The conditions of the electrochemical reduction and oxidation as well as those of thermal demethylation of quaternary benzo(c)-phenanthridine alkaloids namely chelerythrine (1), sanguinarine (2), and fagaronine (3) were studied. Differences in their electrochemical and thermal behavior are correlated with the results obtained from biotransformation studies with liver microsomal fraction.

Alkaloids↗

PubMed1981

Inhibition of liver alanine aminotransferase activity by some benzophenanthridine alkaloids.

The quaternary benzophenanthridine alkaloids sanguinarine (1) and chelerythrine (2) inhibit rat liver L-alanine-:2-oxoglutarate aminotransferase (EC 2.6.1.2) activity. Nitidine (3) has no inhibitory effect. The inhibitory activity of alkaloids depends on the reactivity of the iminium bond with the nucleophilic reagent, e.g., the thiol group. The stability constants of adduct formation for thioethanol, cysteine, and glutathione with sanguinarine (1) and chelerythrine (2) are given. The mechanism of the inhibition of alanine aminotransferase activity by the alkaloids 1 and 2 is discussed.

Alanine Transaminase↗