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Biomedical subjects

J Lansac

Publications and source records attributed to J Lansac.

At least 271 records · Page 15Linked to original sources
PubMedDate not supplied

Analysis of the lipid content and the motility of human sperm after follicular fluid treatment.

The effect of human follicular fluid (hFF) on the cholesterol and phospholipid content and the movement characteristics of human spermatozoa were studied. Semen was selected by a discontinuous Percoll gradient and incubated during in vitro capacitating conditions with B2 medium supplemented with hFF 20%. Percoll pelleted spermatozoa were incubated in either B2 (B2-Percoll) or B2 supplemented with hFF (hFF-Percoll). In hFF-Percoll, we observed a time-dependent (24 h) decrease in both the cholesterol and phospholipid contents (cholesterol: 10.1 vs. 8.7 nmol 10(-7) spermatozoa; phospholipids: 17.5 vs. 15.7 nmol 10(-7) spermatozoa, P < 0.05). This decrease in cholesterol and phospholipids in human spermatozoa was concomitant with a high straight line velocity, a high progressive motility percentage and an increased value of lateral head displacement without any significant alteration of the spermatozoal membrane. No modification of the cholesterol: phospholipid ratio after 2 and 24 h of incubation in either B2-Percoll (0.61, 0.54) in hFF-Percoll (0.59, 0.63) was observed when compared with original control semen. It is suggested that the decrease in cholesterol and phospholipids in hFF-Percoll may be taken into account for the changes of membrane modification as part of the capacitation process.

Cholesterol↗
PubMedDate not supplied

Prenatal diagnosis and postnatal outcome of congenital megalourethra. Report of two cases.

We present 2 cases of congenital megalourethra diagnosed by prenatal ultrasound scan during the second trimester of pregnancy. The first one was associated with features suggesting prune belly syndrome (PBS) in contrast to the other one. Fetal urine biochemistry and fetal serum beta2-microglobulin was used to rule out severe renal impairment that would have led to postnatal renal failure soon after birth. In both cases, after extensive counselling, the parents opted against termination of pregnancy and in both cases vaginal delivery of a live neonate occurred. Postnatal follow-up demonstrated that the two infants were found to have mildly altered renal function without any physical or mental development delay. Association with features of PBS didn't seem to influence the severity of renal function impairment however, but PBS may influence parents' decision regarding termination because of additional corrective surgery and further infertility.

Adult↗
PubMedDate not supplied

[Extrauterine pregnancy and its obstetrical prognosis (apropos of 135 cases)].

The authors have studied 135 case histories of extra-uterine pregnancies which were operated on and followed up the years 1965-1973. They draw attention to: 1. At the clinical level atypical symptoms are found in 29% of cases, which means that the diagnosis is made late in many cases (36% of ruptured tubes) in spite of modern methods of diagnosis. 2. As far as the woman's obstetrical future is concerned they note the low level of further conceptions (51%), the rarity of pregnancies that go to term (24%), and the importance of complication (15%). Fertility after extra-uterine pregnancy is higher when the woman has already had children (43%) as against those women whose extra-uterine pregnancy is in their first pregnancy (25%). They suggest that these results can be improved if on top of surgical treatment further medical supportive treatment is given such as antibiotics, steroids and postoperative hydrotubation.

Female↗
PubMedDate not supplied

Ultrastructural comparison of human spermatozoa along a Percoll density gradient.

We studied the possible deleterious effects of Percoll gradient centrifugation processing on spermatozoal integrity in 18 semen samples from patients participating in our IVF program, using optical and electron microscopy. Substantial recovery by sperm count was obtained in the 100% Percoll layer (32%), with greater numbers of motile, alive spermatozoa (P < .01). Whether samples studied were from fertile or infertile men, no significant differences, using nonparametric tests with transmission electron microscopy, appeared in spermatozoal membranes, acrosomes, or nuclei either before or after Percoll treatment. No deleterious effects of Percoll use on spermatozoa were detected during the study.

Cell Membrane↗
PubMedDate not supplied

[Hormone effects of the administration of 50 mg of dehydroepiandrosterone (DHEAS) intravenously in pregnant women (author's transl)].

The DHEAS overload test has been regarded by C. Lauritzen as reflecting the conditions of placental function. Some will support and some will oppose that test. We have merely concerned ourselves with conducting an examination into the metabolic outcome of the DHEAS overload. --by defining the way in which oestriolury variations are best express themselves. --by estimating how reliable such variations are in cases where foetal pain and hypotrophy are diagnosed. --by going through a statistical analysis of other hormonal variations.

17-Ketosteroids↗