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J L Tullis

Publications and source records attributed to J L Tullis.

64 records · Page 4Linked to original sources
PubMedDate not supplied

Current plasmapheresis practice in the United States.

The principal differences of the USA plasmapheresis collection system from most other countries are: it is conducted by the pharmaceutical industry rather than government, it uses remunerated donors, and it draws a larger volume of plasma than is permitted elsewhere. The implications of these features are reviewed in terms of the effects on donors and the effects on the plasma supply. Protein metabolism is reviewed, and the effects of plasmapheresis on overall nitrogen balance are presented.

Adult↗
PubMedDate not supplied

Precipitation of fibrin monomers and fibrin degradation products by ristocetin.

Ristocetin, at relatively low concentrations (1.0 mg/ml-1.5 mg/ml), can selectively precipitate fibrin monomers and fibrin degradation products (fdp) from plasma without effect on fibrinogen or fibrinogen degradation products (FDP). 125I-labeled fibrin monomers and fibrin degradation products were precipitated by ristocetin when their plasma concentrations were greater than 0.25 microgram/ml and 50 microgram/ml, respectively. In order to obtain a visible precipitated, 2 microgram/ml of fibrin monomers of 50 to 100 microgram/ml of fibrin degradation products were necessary. These effects were optimally observed under the following conditions: (1) temperature, 20 C to 37 C; (2) pH, 7.0 to 7.5; and (3) incubation time, 15 to 60 minutes. Late-fibrin degradation products are approximately eight times less sensitive to ristocetin-induced precipitation than early-fibrin degradation products. Plasma medium is essential for the differentiation of fibrin monomers and fibrin degradation products from fibrinogen and fibrinogen degradation products by ristocetin. These results suggest that the specific detection of fibrin monomers and fibrin degradation products in plasma may be easily performed by ristocetin.

Anticoagulants↗