Biomedical subjects

J L Thompson

Publications and source records attributed to J L Thompson.

At least 109 records · Page 6Linked to original sources

PubMed1982

Pragmatic factors influencing the detection of Neisseria gonorrhoeae.

The effects of the suboptimal temperature caused by delayed incubation or prolonged transport on the recovery of Neisseria gonorrhoeae were studied in 2 separate patient populations. The individual components of the John E. Martin Biological Environmental Chamber were used to define more precisely other variables that might influence the incidence of recovery. In a study of 1500 urethral and endocervical specimens processed in parallel for N gonorrhoeae, results quantitatively and qualitatively superior were achieved when an immediate source of CO2 and a zip-lock bag were used. The zip-lock bag appears to be a significant factor in the enhanced demonstration of N gonorrhoeae. Failure to incubate specimens before transportation decreased the numeric representation of N gonorrhoeae in 50 sets of triple cultures studied in parallel. Most low-inoculum cultures subjected to prolonged transportation without prior incubation will not demonstrate the presence of N gonorrhoeae. Even if preincubated before transportation, the cultures demonstrate a significant reduction in the number of colony-forming units per plate when subjected to normal room temperature during the course of transportation.

Bacteriological Techniques↗

PubMed1981

Proposed standards for professional health sciences library services in hospitals of New York State.

Hospital libraries are considered to be the basic unit of the medical information system. A major statewide effort was begun in 1978 to introduce and support legislation in the New York State Legislature which would encourage hospitals to establish and maintain libraries that meet minimum services standards. Included in this legislation is the concept that the Commissioner of Education in consultation with the Commissioner of Health shall have the power to establish standards for hospital libraries. The Ad Hoc Committee for the Promotion of Hospital Library Services, Western New York Library Resources Council, proposes The Standards for Professional Health Sciences Library Services in Hospitals of New York State to clarify and to strengthen existing hospital library standards. These standards differ specifically from the Joint Commission on Accreditation of Hospitals standards in that they place equal and specific emphasis on eleven points: administration, qualifications of library staff, continuing education of library staff, requirement for a library advisory committee, required library services, required library resources, library space requirements, library budget, library network and consortium membership, documentation of library policy, and continued evaluation of the needs of the hospital for library service. Detailed interpretations are provided. An appendix describes the qualifications of a hospital library consultant.

Libraries, Hospital↗

PubMed1980

Effect of acetazolamide on rate of CO2 uptake in the perfused rat brain.

The accumulation of acid-labile CO2 was measured in the rat brain at early times after exposure to 30% CO2 in oxygen. In particular, the effect on CO2 accumulation of inhibiting brain carbonic anhydrase with acetazolamide was studied. For 57 rats, a rat head perfusion technique was used which permits control and rapid alteration of arterial acid-base conditions. At a paCO2 of 35 mm Hg, total carbon dioxide of the whole brain remained at a normal mean value of 13.4 +/- 0.6 (S.E.) mM/kg during perfusions 209-160 min long. On 30% CO2, brain CO2 increased rapidly to a mean of 24 mM/kg at 5 min and then much more slowly. The latter curve agreed well with values obtained in intact animals. Pretreatment with acetazolamide had no inhibitory effect on CO2 accumulation at 1-5 min. At 5 min, control brain CO2 was 23.9 +/- 0.6 (n = 10) and acetazolamide-pretreated brain CO2 was 24.4 +/- 0.7 (n = 9). Terminal venous pCO2s were 164-180 and 167-172 mm Hg, respectively. Thirty-one unperfused rats were also investigated with similar results. Acetazolamide-pretreated rats had the same CO2 uptake as controls after 15 min exposure to CO2, rather than lower uptake as would be expected if carbonic anhydrase were rate-limiting. The results suggest the need for reinterpretation of some concepts about the role of carbonic anhydrase in CO2 accumulation in the brain.

Acetazolamide↗

PubMed1980

Quantitative and qualitative effects of povidone-iodine liquid and gel on the aerobic and anaerobic flora of the female genital tract.

Povidone-iodine solution (Betadine Solution) produces a dramatic fall in the numbers of total aerobes and anaerobes recoverable from the posterior vaginal pool in the first 10 minutes following administration. Within 30 to 120 minutes, near baseline counts for both aerobic and anaerobic bacteria are re-established. When the vehicle for the povidone-iodine is changed to polyethylene glycol (Betadine Vaginal Gel) an effective antibacterial effect can be documented over a 3 hour period.

Adult↗

PubMed1979

Biological and biochemical observations on isolates of EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas.

Foetal, adult seronegative, and cotton-top marmoset lymphocytes have been transformed into cell lines by EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas. The nature of the cell lines was checked by karyotyping and by light and electron microscopy, and the presence of the EB virus genome was demonstrated by immunofluorescence tests. Immunofluorescence also showed the incidence of EB virus-producing cells in each line and this was checked by electron microscopy and the use of a lymphocyte transformation assay. Two foetal-derived lines did not produce virus spontaneously, could not be activated with various inducers, and were found by DNA reassociation kinetics to carry only small numbers of genome copies per cell. An adult-derived line produced virus, could be activated to produce more, and provided enough infectivity to transform marmoset cells. The resulting marmoset line made profuse transforming virus; it thus provides the first abundant source of NPC-derived infectious EB virus for comparative studies. The results are discussed in relation to the interactions with comparable target cells of EB virus from normal individuals and from patients with other diseases.

Animals↗

PubMed1979

Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude-mouse-passaged nasopharyngeal carcinoma.

After nude-mouse-passage, a carcinoma of the nasopharynx was found to contain a few scattered C-type retrovirus particles. Culture of this nude-mouse-grown material over solid agar allowed the human tumour cells to grow as multicellular spheroids which increased in number by budding and could be subcultured for up to 6 months. Within the spheroids the human tumour cells expressed increased retrovirus replication and large numbers of C-type particles were observed. Treatment with a halogenated pyrimidine further enhanced the virus production. This tissue culture system provided sufficient virus production for the identification of the retrovirus as an endogenous xenotropic murine leukaemia virus and not a human agent.

Animals↗

PubMed1979