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Biomedical subjects

J L Sever

Publications and source records attributed to J L Sever.

At least 271 records · Page 15Linked to original sources
PubMed1969

Viruses and embryos.

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Abnormalities, Multiple↗
PubMed1969

Rubella virus.

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Abnormalities, Multiple↗
PubMed1969

Minicultures of mammalian cells in a new plastic plate.

A new disposable micro tissue culture plate was developed and tested for use in virological procedures. Miniature mammalian cell cultures (minicultures) were grown in these plates. Each plate contained 96 circular cultures in flat wells (7 mm in diameter). Replicate titrations of a number of viruses were performed in various tissues. Excellent reproducibility was demonstrated. Mean infectivity titers determined by miniculture methods were generally within 0.6 log(10)/ml of macro tube titrations. Standard tissue culture assay techniques such as hemadsorption, interference titration, and microneutralization were easily carried out with this method and were very reproducible. Development of this noncytotoxic disposable micro tissue culture plate now permits the routine performance of rapid, reliable, and reproducible tissue culture tests at a very significant reduction in cost and labor.

Adenoviridae↗
PubMed1969

Piggy-back microtransfer technique.

A new microtransfer technique is described which uses a modified, V-bottom, disposable, 96-cup plastic plate that permits serial dilutions and direct rapid transfer to a recipient micro cell culture plate.

Culture Techniques↗